See also: www.cwp-bv.nl www.purepulse.eu

2. Cool Wave Processing
• Develops, manufactures and sells
– PurePulse Equipment (and production lines)
– Novel Cold pasteurisation
– >> 21 days Shelf-Life of fresh juices
– Fresh Taste, High Quality, acceptable prices
– Food Design & consultancy (via TOP b.v. )

3. PEF Background

4. PEF Background
+
+
+
+
+
+
+
+
-
-
-
-
-
-
-
-
Biological cell
1 volt

5. PEF Background
+
+
+
+
+
+
+
+
-
-
-
-
-
-
-
-
Biological cell
1 volt
Pore formation
Cell rupture
Irreversible
Reversible

6. PEF Background
+
+
+
+
+
+
+
+
-
-
-
-
-
-
-
-
Biological cell
1 volt
Pore formation
Cell rupture
Irreversible
ReversibleWe control this
process very well.
We control this
process very well.

7. PEF Background
Pulsed electric field
time
Fieldstrength
Pulse duration (1-30 us)
Repetition rate (10-100Hz)
Low energy process !!!
kV

8. Scale up challenges
Product
properties
Chamber design
(flow and electric
field
optimization)
Generator design
(pulse energy,
field strength,
total power)
Optimal pulse electric field treatment
Lowest energy input -> best Quality!

9. PurePulse = PEF 2.0 from Wageningen
Optimization of flow electric
field and generator design
Cold in-line pasteurisation
• Product temperature << 40°C
• 4-6 log reduction yeast & moulds
• Pilot available up to 600 L/hr
• First commercial system 1200 L/hr

10. PEF 1.0 (current status)
Year 1997
(Labscale USA)

11. Laminar flow
Low temperature-time
Low starting
temperature
HACCP &
Novel Food
approved

12. Product properties – Excellent Quality
Fresh Juice PEF 2.0
• Same perceived taste as fresh-squeezed
• Cloud loss (no inactivation of PME)
• Product temperature << 45°C
• 4-6 log reduction yeast & moulds
• Shelf-life >>21 days @ 7oC
depends on filling: hygienic or aseptic
taste at end shelf-life depends on packaging
properties and food chain conditions

13. No Quality
Benifit !
PEF 1.0 (current status)
Year 1997
(Labscale USA)

14. Pilot Scale facility available for application testing in Wageningen

15. Industrial Scale : 600-1800 L/hr

16. Partners – CWP with PurePulse (PEF
2.0)

18. Industrial Scale : 600-1800 L/hr
PFN-Module Chamber Throughput Installed power
1x size 1 600 L/hr 16 kW
2x size 2 1200 L/hr 30 kW
3x size 3 1800 L/hr 40 kW
Required power connection : <64 A @ 3x400V
Cooling water : 1/2” connection
Compressed air : 8 mm, 6 bar
PurePulse generator (WxDxH) : 1.6 x 1.8 x 2.2 m

19. PurePulse

20. PurePulse
Squeeze the Juice
1

21. PurePulse
Store &
Pump
2

22. PurePulse
Controll of
Temperature
3

23. PurePulse
PurePulse
4

24. PurePulse
CoolDown 5

25. PurePulse
Hygienic Filling
6

26. Conclusions
• PurePulse® is
– the first commercial PEF system
– that does in-line cold pasteurization
– at large industrial scale (>1200 L/hr)
– at product temperatures never exceeding 40°C
– preserving fresh food product characteristics
– with > 21 days shelf life (4-6 log reduction)

27. Why using PurePulse Technology?
PEF 1.0
– Small scale pilot-facilities
– No commercial application
– No scale-up to industrial
throughput’s
– Temperatures of product
are too high (>>60oC)
– No quality benifit
PEF 2.0 - PurePulse
– 500 L/hr available for NPD.
– Low temperature treatment (<40 oC)
– Sufficient shelf-life (>30 days)
– First lines commisioned
– Sustainanble (energy sufficient)
– Excellent, premium product quality
– Price level similar to UHT
– Applications: juices, smoothies,
milk, vegetables

28. High Pressure (HPP)
– Pilot facilities available
– up to 3000 Liter / hr
– Batch process
– No aseptic filling req.
– High investment costs
– Costs per Liter 6-40 cent
– Applications available
PurePulse (PEF)
– 800 L/hr available for NPD.
– Commercial 1200-1800 L / hr
– In-line process
– Aseptic or high-hygenic filling
– Low investment costs
– Costs comparable with UHT
– Applications on the market

29. HPP