This study investigated different bacterial support structures and reactor designs for removing sulphate from acid mine drainage through sulphate reducing bacteria (SRB). Three reactors - a continuous stirred tank reactor (CSTR), laminar flow channel reactor (LFCR), and upflow anaerobic sludge blanket reactor (UASBR) - were evaluated based on their mixing characteristics. Various support matrices were also tested for SRB growth in batch reactors. Results showed that polyurethane foam supported better SRB colonization than other materials. The UASBR with recycling demonstrated mixing between a dynamic and stable system. Going forward, the UASBR may be best for studying SRB using polyurethane foam supports.