The document summarizes an automatic piston discharge centrifuge called the Celeros APD centrifuge. It can provide rotational forces up to 20,000 x G, precision temperature control, and hazardous area designs. It is designed to maximize product purity and yield for applications in biotech manufacturing. The Celeros APD-125 centrifuge has a 5 liter working volume and is built to cGMP standards to operate with minimal intervention, producing the driest paste and clearest centrate available.

1. Rev. 9/13/2011
• Rotational forces up to 20,000 x G
• PLC control for process flexibility
• Precision temperature control
• Hazardous area and C EC EC EC E designs available
• Vacuum system available
• Variety of paste recovery methods
APDAPD--125125
The Biocentrifuge...when every product gram matters!
Yield
• High separation efficiency
• cGMP construction
• Closed process from feed to discharge
• Full CIP and SIP capabilities
• Total recovery of valuable product
• Complete discharge of dry paste
• Designed to maximize centrate recovery
• Low shear forces for sensitive products
Automatic Piston Discharge CentrifugeAutomatic Piston Discharge Centrifuge
Purity
Versatility
If your goal in a centrifugation step is to maximize product purity and yield, choose the versatile Celeros APD
centrifuge which is setting new standards for harvesting and clarification.
Combining up to 20,000 x G of separating force and the patented, fully Automatic Piston Discharge (APD)
system, the Celeros APD centrifuge produces the driest paste and clearest centrate of any industrial centrifuge
available today for the biotech industry.
The Celeros APD-125 has a 5 liter working volume and provides the highest purity, yield, and versatility available
in a sanitary industrial centrifuge. It is built to cGMP standards and designed to operate with minimal operator
intervention.

2. Rev. 9/13/2011
Technical Specifications:
Process Applications:
Celeros Separations
tel: 508-698-3080 www.celerosinc.com email: solutions@celerosinc.com
With an ever-increasing range of therapeutic molecules produced by a variety of host cells, the challenge of
efficiently executing liquid / solid separations in biotech manufacturing processes has never been greater. Product
losses during these steps can be substantial.
The Celeros APD centrifuge has been specifically designed to meet this challenge. The ability to precisely control
rotational speed, feed flow, and temperature in a machine that can produce and completely discharge dry solids and
clear centrate is changing industry expectations for centrifuge performance.
The APD centrifuge is currently used in the production of a variety of vaccines and other therapeutics. Its versatility
enables the same machine to handle a variety of applications including:
Whole cell separation – Bacterial, fungal, mammalian, insect, et al.
• Unique ability to handle high density cultures in a single step (tested at up to 50% solids!)
Cell debris removal – after chemical/mechanical lysis or protein precipitation step
• Unique ability to separate and discharge inclusion bodies after multiple wash steps
Blood plasma fractionation – optimized for several steps in both warm and cold fractionation processes.
Collect “dry” paste/solids like
never before!*Actual process flows vary based upon application.
Separation Force 0 - 20,000 x G
Operating Temperature -10° to 40° C
Working Bowl Volume 5 liters
*Max Flow (water) 7 lpm
Dimensions 2,600 mm x 1,350 mm x 1,100 mm
Weight (empty) 850 kg
How does the Celeros APD centrifuge technology maximize recovery?
Under rotation, bowl
fills from bottom.
Clear centrate exits the
bowl through a valve in
the piston.
Paste is retained on
bowl wall.
Bowl stops rotating
piston discharges paste/
retained solids.
Piston completes
discharge!