Microscopy techniques for observing the transparent nematode C. elegans are described. Phase contrast microscopy allows details in C. elegans living cells and tissues to appear darker or brighter against a background, improving visibility. The methodology involved collecting soil samples from a botany garden, placing them on a petri dish, using a dissecting microscope to find C. elegans, transferring them to a slide, then viewing under a light microscope at 100x and 400x magnification to take pictures.

1. Microscopy Techniques
By
Carolina Montañez

2. Objective
• C. elegans
 Transparent nematode
 Found in soil
 About 1mm in length
• Phase Contrast Technique
 bright-field
 details in the image appear darker/brighter against a
background
 transparent specimen, such as living cells and
microorganisms

3. Methodology
1. Soil in the botany garden
2. Petridish
3. Dissecting microscope
4. Slide
5. Light microscope
6. Took some pictures

4. C. Elegans
Nematodes in soil

5. 100x

6. 400X