Can time lapse incubation and monitoring be beneficial for art monitoring in ART 2022.pptx
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After close to 45 years of assisted reproduction and a glance back at what are major milestones had impacted the practice of assisted reproduction. The first is the birth of Louis brown in 1978. A decade and a half later, the advent of icsi, tese icsi, has changed the management of male factor infertility. A decade later, in the early 2000s, we had the vitrification initially of the embryos, latter oocyte, and reproductive tissue. Now maybe we are. on the threshold of another revolution, and this time into ivf laboratory where the closed door of embryo development has to be opened to the miracle of early human embryology development through time-lapse embryo monitoring. This presentation walks you through the development
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Can time lapse incubation and monitoring be beneficial for art monitoring in ART 2022.pptx
- 1. Can time-lapse incubation and monitoring be beneficial for art DR G A RAMARAJU
- 2. MAJOR CHANGES IN ASSISTED REPRODUCTION IVF ICSI TESE VITRIFICATION ? TIME LAPSE MONITORING ?
- 4. Lewis & Gregory, 1929 (Science, 69) •
- 5. Single-point morphological assessment Blastocyst culture D3 culture Continuous embryo monitoring Manual algorithms Automated image analysis platforms Evolution of non-invasive methods for embryo assessment 20112 2018 19971 1Payne et al. Hum Reprod. 1997;12:532–541. 2Meseguer et al. Hum Reprod. 2011;26:2658–2671. EVOLUTION OF HUMAN EMBRYO MONITORING
- 7. Conventional incubators and it variables
- 8. Time-lapse Incubator Holds 6 cultures at a time Temperature control using inbuilt sensors. 11 plane HD imaging system for every 5 minutes interval. optimal culture conditions Light: Long wavelength light reduce light- induced damage to early stage of embryo development humidified
- 12. Description of recorded morphokinetic events first 30 hours Hum Reprod, Volume 34, Issue 8, August 2019, Pages 1439–1449,
- 13. New parameter of embryo viability
- 14. Temporal distributions of morphokinetics events occurring during fertilization and relevant to meiotic ... Hum Reprod, Volume 33, Issue 1, January 2018, Pages 23–31
- 15. Timeline of embryo development
- 16. The minimum and maximum data rate of embryo growth over 100 hours to 120 hours
- 17. The critical learning of time lapse Change in fertilization check timing Missing abnormal chevages •direct cleavage reverse chevage Delays in cell division timing are applicable in blastocyst prediction.
- 18. The optimum time to perform fertilization assessment for oocytes cultured in standard incubation is 16.5 § 0.5 h post-insemination (hpi). The current consensus requires modification to minimize the chance of fertilization being missed. 8.6% of ICSI-derived zygotes and 10% of IVF- derived zygotes are likely to be classified as unfertilised.
- 20. Abnormal considered as normal and transferred in a typical IVF lab TIME LAPSE IDENTIFIES THEM +
- 21. Direct cleavage
- 22. Reverse cleavage
- 23. 3 PN 3 pn
- 24. Based on the rate of cell division, evolving information suggests euploid mosaic and blastocyst can be picked up. 2021.
- 25. Distribution of morphokinetic timing in low- and high- degree mosaics Distribution of Morphokinetic timing
- 26. The benefits of a time-lapse Incubator system Better culture conditions More information
- 27. Based on the evidence presented : Brings Embryology into the clinic • Demystifies embryology
- 28. THANK YOU
Editor's Notes
- 20 min
- TIME LAPSE INCUBATOR WAS DEVELOPED IN 2008 AND LAUNCHED IN USA IN 2011 SINCE THEN THERE IS GRADUAL INCREASE IN USSAGE AND ADDITION OF TECHNOLOGY
- Figure 1 Description of recorded morphokinetic events (A) and monitored morphologic-morphometric parameters (B). Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- Figure 1 Temporal distributions of morphokinetics events occurring during fertilization and relevant to meiotic resumption, pronuclear dynamics, chromatin organization and cytoplasmic/cortical modifications. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com