. Time-lapse embryo monitoring is a compact modular benchtop incubator that incorporates time-lapse imaging to capture critical developmental stages of embryo development. Designed to offer an individualized and undisturbed incubation, the time-lapse incubator provides stable culture conditions in an optimal environment.#geri #krishnaivf

1. Current trends in embryology: Role
of Timelapse-embryo monitoring
• DR G A RAMA RAJU

2. MAJOR CHANGES IN ASSISTED
REPRODUCTION
IVF
ICSI
TESE
VITRIFICATION
Preimplantation
Genetic
Diagnosis
? TIME LAPSE
MONITORING
?

4. Lewis & Gregory, 1929 (Science, 69) •

5. Single-point
morphological assessment
Blastocyst culture
D3 culture
Continuous embryo
monitoring Manual algorithms Automated image
analysis platforms
Evolution of non-invasive methods for embryo assessment
20112 2018
19971
1Payne et al. Hum Reprod. 1997;12:532–541.
2Meseguer et al. Hum Reprod. 2011;26:2658–2671.
EVOLUTION OF HUMAN EMBRYO
MONITORING

6. EARLIEST HUMAN INCUBATOR

7. Conventional incubators and it variables

8. Time-lapse Incubator
Holds 6 cultures at a time
Temperature control using inbuilt sensors.
11 plane HD imaging system for every 5
minutes interval.
optimal culture conditions
Light: Long wavelength light reduce light-
induced damage to early stage of embryo
development
humidified

9. Precisión Embryology.

10. FACTOR AFFECTING CLEAVAGE

11. FACTOR AFFECTING CLEAVAGE

12. FACTOR AFFECTING CLEAVAGE

13. Conventional embryo observation

14. The
critical
learning
of
time
lapse Change in
fertilization
check timing
Missing
abnormal
chevages
•direct cleavage
reverse
chevage
Delays in cell
division
timing are
applicable in
blastocyst
prediction.

15. New parameter of embryo viability

16. Temporal
distributions of
morphokinetics
events occurring
during fertilization
and relevant to
meiotic ...
Hum Reprod, Volume 33, Issue 1, January 2018, Pages 23–31

17. Description of recorded morphokinetic events first 30 hours
Hum Reprod, Volume 34, Issue 8, August 2019, Pages 1439–1449,

18. Time-lapse is
making
understandin
g embryos
better.

19. Timeline of embryo development

20. The minimum and maximum data rate of embryo growth over 100 hours
to 120 hours

21. The optimum time to perform fertilization assessment for oocytes cultured in standard incubation is 16.5 § 0.5 h post-insemination (hpi). The
current consensus requires modification to minimize the chance of fertilization being missed.
8.6% of ICSI-derived
zygotes and 10% of IVF-
derived zygotes are likely
to be classified as
unfertilised.

22. Abnormal
considered as
normal and
transferred in a
typical IVF lab
TIME LAPSE IDENTIFIES THEM
+

23. Direct cleavage

24. Reverse cleavage

25. 3 PN
3 pn

26. Based on the rate of cell division, evolving information
suggests euploid mosaic and blastocyst can be picked up.
2021.

27. Distribution of Morphokinetic timing
Distribution of morphokinetic timing in low- and high-
degree mosaics

28. The benefits of a time-lapse Incubator system
Better culture
conditions
More
information

29. Based on the evidence presented :
Brings Embryology into the clinic •
Demystifies embryology

30. THANK YOU