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SCIENTIFIC
How to know     EXPLANATION
                                                        PROCEDURE:
 if water is    The DBC reagent containing the
                chromogenic substrate

    clean?      enzyme 5-bromo-4-chloro-3-
                indolyl-B-D galactopyranoside
                (X-GAL)                                         • Take the sample
                for the detection of ß-                           to the flask to
                galactosidase (an enzyme
                                                       Step 1     100ml.

  -Andrea F.    indicative of
                coliform). In the hydrolysis of ß-

       #9       Dgalactosidasa.
                X-GAL off a chromogenic                         • Pour the
                compound (indigo-blue)                            contents of the
  -Alicia Rdz   makes the medium turns from                       powder in the

      #23
                yellow to blue-green.
                It also contains the fluorogenic
                                                       Step 2     vial with the
                                                                  sample.
                substrate of the enzyme
 -Pamela Mtz    4-methyl-umbelliferil-bd-
                glucuronide (MUG) for                           • Stir until the

      # 21
                                                                  powder
                detecting                                         completely
                ß-glucuronidase (an enzyme                        integrated.Incubat
-Cristina Gzz   specific for E. coli). In hydrolysis
                ß-glucuronidase, MUG clear 4-
                                                       Step 3     e at 35 2 ° C from
                                                                  18 to 24 hours.

      #13       methylumbelliferone
                which fluoresces when exposed

-Andrea Hdz     to ultraviolet light.
                The fluorescence distinguishes
                the presence of E. coli group
      #16       coliform.
                Take the sample in the vial
What it is              What is going to
about:                  happen is that the
Selective enrichment    BCD powder
medium and              suppose to
differential            identify if the
for the detection of    water is clean or is
microorganisms of the
group
                        not.
coliforms in various
products and to         Conclusions:
investigate             If after all the
the presence of E.      procedure the
coli by fluorescence    water is green it
and testing
                        means is not
confirmatory by
indole.                 clean but if its
                        yellow is clean.

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  • 1. SCIENTIFIC How to know EXPLANATION PROCEDURE: if water is The DBC reagent containing the chromogenic substrate clean? enzyme 5-bromo-4-chloro-3- indolyl-B-D galactopyranoside (X-GAL) • Take the sample for the detection of ß- to the flask to galactosidase (an enzyme Step 1 100ml. -Andrea F. indicative of coliform). In the hydrolysis of ß- #9 Dgalactosidasa. X-GAL off a chromogenic • Pour the compound (indigo-blue) contents of the -Alicia Rdz makes the medium turns from powder in the #23 yellow to blue-green. It also contains the fluorogenic Step 2 vial with the sample. substrate of the enzyme -Pamela Mtz 4-methyl-umbelliferil-bd- glucuronide (MUG) for • Stir until the # 21 powder detecting completely ß-glucuronidase (an enzyme integrated.Incubat -Cristina Gzz specific for E. coli). In hydrolysis ß-glucuronidase, MUG clear 4- Step 3 e at 35 2 ° C from 18 to 24 hours. #13 methylumbelliferone which fluoresces when exposed -Andrea Hdz to ultraviolet light. The fluorescence distinguishes the presence of E. coli group #16 coliform. Take the sample in the vial
  • 2. What it is What is going to about: happen is that the Selective enrichment BCD powder medium and suppose to differential identify if the for the detection of water is clean or is microorganisms of the group not. coliforms in various products and to Conclusions: investigate If after all the the presence of E. procedure the coli by fluorescence water is green it and testing means is not confirmatory by indole. clean but if its yellow is clean.