Call Girls Service Pune Vaishnavi 9907093804 Short 1500 Night 6000 Best call ...
Bio project.pdf
1.
INTRODUCTION
What is Biotechnology?
Biotechnology is the use of living systems and
organisms to develop or make products, or "any
technological application that uses biological
systems, living organisms or derivatives thereof,
to make or modify products or processes for
specific use
At its simplest, biotechnology is technology
based on biology - biotechnology harnesses
cellular and bio molecular processes to develop
technologies and products that help improve
our lives and the health of our planet. We have
used the biological processes of
microorganisms for more than 6,000 years to
make useful food products, such as bread and
cheese, and to preserve dairy products
2.
Modern biotechnology provides breakthrough
products and technologies to combat
debilitating and rare diseases, reduce our
environmental footprint, feed the hungry,
useless and cleaner energy, and have safer,
cleaner and more efficient industrial
manufacturing processes. Biotech is helping to
heal the world by harnessing nature's own
toolbox and using our own genetic makeup to
heal and guidelines of research by:
●
Reducing rates of infectious disease
●
Saving millions of children's lives
●
Changing the odds of serious, life-threatening
conditions affecting millions around the world
●
Tailoring treatments to individuals to minimize
health risks and side effects
●
Creating more precise tools for disease detection
Combating serious illnesses and everyday
threats confronting the developing world
.
3.
HISTORY
Throughout the history of agriculture, farmers have
inadvertently altered the genetics of their crops through
introducing them to new environments and breeding them with
other plants - one of the first forms of biotechnology. These
processes also were included in early fermentation of beer. In
brewing, malted grains (containing enzymes) convert starch
from grains into sugar and then adding specific yeasts to
produce beer. In this process, carbohydrates in the grains were
broken down into alcohols such as ethanol. Later other cultures
produced the process of lactic acid fermentation which allowed
the fermentation and preservation of other forms of food, such
as soy sauce. Fermentation was also used in this time period to
produce leavened bread. Although the process of fermentation
was not fully understood until Louis Pasteur's work in 1857, it is
still the first use of biotechnology to convert a food source into
another form.
4.
For thousands of years, humans have used selective breeding to
improve production of crops and livestock to use them for food.
In selective breeding, organisms with desirable characteristics
are mated to produce offspring with the same characteristics.
For example, this technique was used with corn to produce the
largest and sweetest crops. Biotechnology has also led to the
development of antibiotics. In 1928, Alexander Fleming
discovered the mould Penicillium. His work led to the
purification of the antibiotic compound formed by the mould by
Howard Florey, Ernst Boris Chain and Norman Heatley - to form
what we today know as penicillin. In 1940, penicillin became
available for medicinal use to treat bacterial infections in
humans.
The field of modern biotechnology is generally thought of as
having been born in 1971 when Paul Berg's experiments in gene
splicing had early success. Herbert W. Boyer and Stanley N.
Cohen significantly advanced the new technology in 1972 by
transferring genetic material into a bacterium, such that the
imported material would be reproduced
.
5.
BIOTECHNOLOGY IN AGRICULTURE
Genetically Modified Crops:
Genetically modified crops
or “GM crops” or “biotech crops” are plants
used in agriculture, the DNA of which has been modified with genetic
engineering techniques. In most cases the aim is to introduce a new trait
to the plant which does not occur naturally in the species. Examples in
food crops include resistance to certain pests, diseases, stressful
environmental conditions, resistance to chemical treatments, reduction of
spoilage, or improving the nutrient profile of the crop.
Examples in non-food crops include production of pharmaceutical agents,
bio fuels, and other industrially useful goods, as well as for
bioremediation.
Plants and crops with GM traits have been tested more than any other
crops—with no credible evidence of harm to humans or animals. In fact,
seeds with GM traits have been tested more than any other crops in the
history of agriculture – with no credible evidence of harm to humans or
animals
6.
RNA Interference (RNAi):
RNA interference (RNAi) is a method of blocking gene function by
inserting short sequences of ribonucleic acid (RNA) that match part of the
target gene’s sequence, thus no proteins are produced. RNAi has the
potential to become a powerful therapeutic approach toward targeted
and personalized medicine. RNAi has provided a way to control pests and
diseases, introduce novel plant traits and increase crop yield. Using RNAi,
scientists have developed novel crops such as nicotine-free tobacco,
non-allergenic peanuts, decaffeinated coffee, and nutrient fortified maize
among many others.
RNA interference (RNAi) has recently been demonstrated in plant
parasitic nematodes. It is a potentially powerful investigative tool for the
genome-wide identification of gene function that should help improve our
understanding of plant parasitic nematodes. RNAi should help identify
gene and, hence, protein targets for nematode control strategies.
Prospects for novel resistance depend on the plant generating an
effective form of double-stranded RNA in the absence of an endogenous
target gene without detriment to itself. These RNA molecules must then
become available to the nematode and be capable of ingestion via its
feeding tube. If these requirements can be met, crop resistance could be
achieved by a plant delivering a dsRNA that targets a nematode gene
and induces a lethal or highly damaging RNAi effect on the parasite
7.
Bt toxin:
A protein that is toxic to chewing insects and is produced by the soil
bacterium Bacillus thuringiensis and has long been used as a biological
pesticide. By means of genetic engineering, the genes for Bt toxin can be
isolated from Bacillus thuringiensis and transferred to plants. Bacillus
thuringiensis (Bt) is a bacteria that produces proteins which are toxic to
insects. But extreme toxicity comes at no surprise. It’s in the same family
of bacteria as B. anthracis, which causes anthrax, and B. cereus, which
causes food poisoning.
The Bt toxin dissolve in the high pH insect gut and becomes active. The
toxins then attack the gut cells of the insect, punching holes in the lining.
The Bt spores spills out of the gut and germinate in the insect causing
death within a couple days. Even though the toxin does not kill the insect
immediately, treated plant parts will not be damaged because the insect
stops feeding within hours. Bt spores do not spread to other insects or
cause disease outbreaks on their own
1
. Insect eats Bt crystals and spores.
2.
The toxin binds to specific receptors in the gut and the insects stops
eating
3.
The crystals cause the gut wall to break down, allowing spores and
normal gut bacteria to enter the body.
4
. The insect dies as spores and gut bacteria proliferate in the body
8.
Bt Cotton:
Bt cotton is a genetically modified organism (GMO) cotton variety, which
produces an insecticide to bollworm. Strains of the bacterium Bacillus
thuringiensis produce over 200 different Bt toxins, each harmful to
different insects. Most notably, Bt toxins are insecticidal to the larvae of
moths and butterflies, beetles, cotton bollworms and ghtu flies but are
harmless to other forms of life. The gene coding for Bt toxin has been
inserted into cotton as a transgene, causing it to produce this natural
insecticide in its tissues. In many regions, the main pests in commercial
cotton are lepidopteran larvae, which are killed by the Bt protein in the
genetically modified cotton they eat. This eliminates the need to use large
amounts of broad-spectrum insecticides to kill lepidopteran pests. This
spares natural insect predators in the farm ecology and further
contributes to non insecticide pest management.
Bt cotton is ineffective against many cotton pests such as plant bugs,
stink bugs, and aphids; depending on circumstances it may be desirable
to use insecticides in prevention. A 2006 study done by Cornell
researchers, the Center for Chinese Agricultural Policy and the Chinese
Academy of Science on Bt cotton farming in China found that after seven
years these secondary pests that were normally controlled by pesticide
had increased, necessitating the use of pesticides at similar levels to
non-Bt cotton and causing less profit for farmers because of the extra
expense of GM seeds.
9.
MECHANISM:
Bt cotton was created through the addition of genes encoding toxin
crystals in the Cry group of endotoxin. When insects attack and eat the
cotton plant the Cry toxins are dissolved due to the high pH level of the
insects' stomachs. The dissolved and activated Cry molecules bond to
cadherin-like proteins on cells comprising the brush border
molecules. The epithelium of the brush border membranes separates
the body cavity from the gut whilst allowing access for nutrients. The
Cry toxin molecules attach themselves to specific locations on the
cadherin-like proteins present on the epithelial cells of the midge and
ion channels are formed which allow the flow of potassium. Regulation
of potassium concentration is essential and, if left unchecked, causes
death of cells. Due to the formation of Cry ion channels sufficient
regulation of potassium ions is lost and results in the death of epithelial
cells. The death of such cells creates gaps in the brush border membrane
.
ADVANTAGES:
Bt cotton has several advantages over non Bt cotton. The important
advantages of Bt cotton are briefly :
●
Increases yield of cotton due to effective control of three types of
bollworms, viz. American, Spotted and Pink bollworms.
●
Insects belonging to Lepidoptera (Bollworms) are sensitive to crystalline
endotoxic protein produced by the Bt gene which in turn protects cotton
from bollworms.
●
Reduction in pesticide use in the cultivation of Bt cotton in which
bollworms are major pests.
●
Reduction in the cost of cultivation and lower farming risks.
●
Reduction in environmental pollution by the use of insecticides rarely.
●
Bt cotton exhibits genetic resistance or inbuilt resistance which is a
permanent type of resistance and not affected by environmental factors
10.
DISADVANTAGES:
Bt cotton has some limitations
:
●
High cost of Bt cotton seeds as compared to non Bt cotton seeds.
●
Effectiveness up to 120 days, after that the toxin producing efficiency of
the Bt gene drastically reduces.
●
Ineffective against sucking pests like jassids, aphids, whitefly etc.
BT COTTON IN INDIA:
Bt cotton is supplied in India's Maharashtra state by the
agribiotechnology company, Mahyco, as the distributor.
The use of Bt cotton in India has grown exponentially since its
introduction. Recently India has become the number one global exporter
of cotton and the second largest cotton producer in the world. India has
bred Bt-cotton varieties such as Bikaneri Nerma and hybrids such as
NHH-44, setting up India to benefit now and well into the future.
India’s success has been subject to scrutiny. Monsanto's seeds are
expensive and lose vigor after one generation, prompting the Indian
Council of Agricultural Research to develop a cheaper Bt cotton variety
with seeds that could be reused.
11.
The state of Maharashtra banned the sale and distribution of Bt cotton in
2012, to promote local Indian seeds, which demand less water, fertilizers
and pesticide input, but lifted the ban in 2013.
India approved Bt cotton in 2002; now it accounts for 92% of all Indian
cotton. Average nationwide cotton yields went from 302 kg/ha in the
2002/3 season to a projected 481 kg/ha in 2011/12 — up 59.3% overall.
This chart shows the trends in yields, which took off after Bt was
introduced in 2002. The graphs also show that — and here comes ugly
fact— in the last 4 years, as Bt has risen from 67% to 92% of India’s
cotton, yields have dropped steadily.
12.
BIOTECHNOLOGY IN MEDICINES
Genetically Engineered Insulin (Humulin):
Insulin is a peptide hormone produced by beta cells in the pancreas of
various organisms including human beings. It regulates the metabolism of
carbohydrates and fats by promoting the absorption of glucose from the
blood to skeletal muscles and fat tissue and by causing fat to be stored
rather than used for energy. Insulin also inhibits the production of glucose
by the liver.
Except in the presence of the metabolic disorder diabetes mellitus and
metabolic syndrome, insulin is provided within the body in a constant
proportion to remove excess glucose from the blood, which otherwise
would be toxic. When blood glucose levels fall below a certain level, the
body begins to use stored glucose as an energy source through
glycogenolysis, which breaks down the glycogen stored in the liver and
muscles into glucose, which can then be utilized as an energy source. As a
central metabolic control mechanism, its status is also used as a control
signal to other body systems (such as amino acid uptake by body cells).
In addition, it has several other anabolic effects throughout the body.
When control of insulin levels fails, diabetes mellitus can result.
13.
HUMULIN:
Humulin was the first medication produced using modern genetic
engineering techniques in which actual human DNA is inserted into a host
cell (E. coli in this case). Biosynthetic "human" insulin is now manufactured
for widespread clinical use using genetic engineering techniques using
recombinant DNA technology, which the manufacturers claim reduces the
presence of many impurities, although there is no clinical evidence to
substantiate this claim. Eli Lilly marketed the first artificial insulin,
Humulin, in 1982.
Humulin production method is as follows:
1
. DNA coding for A and B polypeptide chains of insulin are chemically
synthesised in the lab. Sixty three nucleotides are sequenced to produce A
chain of insulin and ninety nucleotide long DNA designed to produce B
chain of insulin, plus terminator codon is added at the end of each chain
sequence. Anti-codon for methionine is added at the beginning of the
sequence to distinguish humulin from the other bacterial proteins.
2.
Chemically synthesized A and B chain DNA sequence are inserted into
one of the marker gene which are present in the plasmid vector. Genes are
inserted into the plasmid with the help of enzymes known as
endonuclease and ligase.
3.
The vector plasmids with the insulin gene are then introduced into the
E. coli bacterial cell. These cells are then allowed to replicate by mitosis,
along with the bacterial cell recombinant plasmid also gets replicated
producing the human insulin.
4.
A and B polypeptide chains of insulin are then extracted and purified
from the fomenters in the lab. High-Performance Liquid Chromatography
(HPLC) is used to get 100% pure humulin from the mixture of proteins.
5.
The A and B polypeptide chains of insulin are mixed together and
connected with each other by disulphide bond, forming the Humulin or
synthetic human insulin
14.
ADVANTAGES & DISADVANTAGES OF HUMULIN:
Humulin is the one and only human protein produced in the bacteria with
identical chemical structure to that of the natural human insulin.
Administration of humulin reduces the possibility of antibody production
and inflammatory response in diabetic patients. Major difficulty is the
extraction of humulin from a mixture of host proteins present in the
fermentation broth.
Nowadays to overcome this extraction problem synthetic human insulin is
produced in the yeast cell instead of E. coli using the same procedure. As
yeast is Eukaryotes they secrete the whole humulin molecule with perfect
three dimensional structures, reducing the need for complex and time
consuming purification methods.
Now most of the diabetic patients are treated with synthetic human
insulin. Small group of patients claim that episodes of hyperglycaemic
complications have been increased after shifting from animal origin
insulin to humulin. No study till date shows the difference between the
frequency of hyperglycaemic complications in patient using humulin
(synthetic human insulin) and animal origin insulin.
15.
Gene Therapy:
Gene therapy is the therapeutic delivery of nucleic acid polymers into a
patient's cells as a drug to treat disease. Gene therapy is an experimental
technique that uses genes to treat or prevent disease. In the future, this
technique may allow doctors to treat a disorder by inserting a gene into a
patient’s cells instead of using drugs or surgery. Researchers are testing
several approaches to gene therapy, including:
●
Replacing a mutated gene that causes disease with a healthy copy
of the gene.
●
Inactivating, or “knocking out,” a mutated gene that is functioning
improperly.
●
Introducing a new gene into the body to help fight a disease.
16.
Although gene therapy is a promising treatment option for a number of
diseases (including inherited disorders, some types of cancer, and certain
viral infections), the technique remains risky and is still under study to
make sure that it will be safe and effective. Gene therapy is currently only
being tested for the treatment of diseases that have no other cures. It
should be noted that not all medical procedures that introduce alterations
to a patient's genetic makeup can be considered gene therapy. Bone
marrow transplantation, and organ transplants in general have been
found to introduce foreign DNA into patients. Gene therapy is defined by
the precision of the procedure and the intention of direct therapeutic
effects.
Gene therapy was conceptualized in 1972, by authors who urged caution
before commencing human gene therapy studies.
The first attempt, albeit an unsuccessful one, at gene therapy (as well as
the first case of medical transfer of foreign genes into humans not
counting organ transplantation) was performed by Martin Cline on 10 July
1980. Cline claimed that one of the genes in his patients was active six
months later, though he never published this data or had it verified and
even if he is correct, it's unlikely it produced any significant beneficial
effects treating beta-thalassemia.
The first germ line gene therapy consisted of producing a genetically
engineered embryo in October 1996. The baby was born on July 21, 1997
and was produced by taking a donor's egg with healthy mitochondria,
removing its nuclear DNA and filling it with the nuclear DNA of the
biological mother - a procedure known as cytoplasmic transfer.
This procedure was referred to sensationally and somewhat inaccurately
in the media as a "three parent baby", though mtDNA is not the primary
human genome and has little effect on an organism's individual
characteristics beyond powering their cells.
17.
Gene therapy is a way to fix a genetic problem at its source. The polymers
are either expressed as proteins, interfere with protein expression, or
possibly correct genetic mutations.
The most common form uses DNA that encodes a functional, therapeutic
gene to replace a mutated gene. The polymer molecule is packaged
within a "vector", which carries the molecule inside cells.
The first commercial gene therapy, Gendicine, was approved in China in
2003 for the treatment of certain cancers. In 2011 Neovasculgen was
registered in Russia as the first-in-class gene-therapy drug for treatment
of peripheral artery disease, including critical limb ischemia. In 2012
Glybera, a treatment for a rare inherited disorder, became the first
treatment to be approved for clinical use in either Europe or the United
States after its endorsement by the European Commission.
ADA deficiency is one form of SCID (severe combined immunodeficiency),
a disorder that affects the immune system. ADA deficiency is very rare,
but very dangerous, because a malfunctioning immune system leaves the
body open to infection from bacteria and viruses.
18.
The disease is caused by a mutation in a gene on chromosome 20. ADA
deficiency is inherited in an autosomal recessive manner. The gene codes
for the enzyme adenosine deaminase (ADA). Without this enzyme, the
body is unable to break down a toxic substance called deoxyadenosine.
The toxin builds up and destroys infection-fighting immune cells called T
and B lymphocytes. Because ADA deficiency affects the immune system,
people who have the disorder are more susceptible to all kinds of
infections, particularly those of the skin, respiratory system, and
gastrointestinal tract. They may also be shorter than normal. Sadly, most
babies who are born with the disorder die within a few months
Treatments of ADA Deficiency
includes:
●
bone marrow transplant
●
gene therapy
●
ADA enzyme in PEG vehicle
On September 14, 1990, the first gene therapy to combat this disease was
performed by Dr. William French Anderson on a four-year old girl, Ashanti
DeSilva, at the National Institutes of Health, Bethesda, Maryland, U.S.A.
19.
CONCLUSION
Biotechnology is the new wonder of science. It is truly multidisciplinary
in nature and it encompasses several disciplines of basic sciences and
engineering. The Science disciplines from which biotechnology draws
heavily are microbiology, chemistry, biochemistry, genetics, molecular
biology, immunology, cell and tissue culture and physiology. On the
engineering side it leans heavily on process chemical and biochemical
engineering since large scale cultivation of microorganisms and cells,
their downstream processing is based on them. It comes to us as a
great blessing...
Biotechnology utilizes the technique called genetic engineering or
recombinant DNA technology where a microorganism is isolated; its
genetic material is cut, manipulated, sealed, again inserted in an
organism and allowed to grow in a suitable environment under
controlled conditions to get the desired product. It looks easy but is a
very tedious job and it takes years for research to achieve its goal.
The applications of biotechnology are so broad, and the advantages so
compelling, that virtually every industry is using this technology.
Developments are underway in areas as diverse as pharmaceuticals,
diagnostics, textiles, aquaculture, forestry, chemicals, household
products, environmental cleanup, food processing and forensics to name
a few. Biotechnology is enabling these industries to make new or better
products, often with greater speed, efficiency and flexibility.
Biotechnology must continue to be carefully regulated so that the
maximum benefits are received with the least risk.
23.
OBJECTIVE
The purpose of the experiment - Study
Recombinant Dna Technology In Today’s Medicine
The objective of exploring Recombinant DNA
Technology in today's medicine is to comprehensively
examine the profound impact and transformative
role this cutting-edge biotechnological tool plays in
the field of healthcare. By delving into the
fundamental principles, historical development, and
key components of Recombinant DNA Technology,
the aim is to provide a clear understanding of its
underpinning mechanisms.