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1. Bio Distribution of Feridex in
Apo-E Deficient and
C57BL/6(normal) Mice

2. Study Design
7 A p o - E
a n d
4 C 5 7 B l
M ic e
H & E , P e a r l's ( ir o n ) ,
C D 6 8 , M o v a ts
S ta in in g
S a c r if ic e d in D a y s 1 ,3 ,5
3 m M o lF e /k g
In je c te d In tr a v e n o u s ly
S a c r ific e d in D a y s 1 ,3 ,5
H & E , P e a r l's ( ir o n ) ,
C D 6 8 , M o v a ts
S ta in in g
1 m M o lF e /k g
In je c te d In tr a v e n o u s ly

3. ApoE Mouse Died 3hours
After Injection, Ascending Aorta
H&E Pearl’s

4. ApoE Mouse Died 3hours
After Injection, Ascending Aorta
Pearl’s Immunostaining

5. ApoE Mouse Died 3hours
After Injection, Aorta and a small artery
H&E Pearl’s

6. ApoE Mouse Died 3hours
After Injection,
Pearl’s Immunostaining

7. ApoE, High Dose, Day1, Aortic Valve
H&E Pearl’s

8. ApoE, High Dose, Day1
Pearl’s Immunostaining

9. ApoE, Low Dose, Day1,
Aortic Valve
H&E Pearl’s

10. ApoE, Low Dose, Day1 Valve
Pearl’s Immunostaining

11. ApoE, High Dose, Day1, Ascending
H&E Pearl’s

12. ApoE, Low Dose, Day3,
Descending Aorta(no plaque!)
H&E Descending Aorta Pearl’s

13. ApoE, Low Dose, Day3
No plaque, No Iron
Pearl’s

14. ApoE, High Dose, Day5
H&E Pearl’s

15. ApoE, High Dose, Day5

16. ApoE, Low Dose, Day5
H&E Pearl’s

17. ApoE, Low Dose, Day5

18. Normal Mouse, Day2
Ascending Aorta
H&E Pearl’s

19. C57BL/6, Day2,
(3 Particle, Just Under the Endothelium)
Pearl’s Immunostaining

20. Normal Mouse, Day2
H&E Pearl’s

21. Normal Mouse, Day 5
H&E Pearl’s
No Iron

22. Normal Mouse,
without Plaque, One Iron particle

23. Normal Mice
Comparing to numerous iron
particles seen in ApoE aortic
atherosclerotic plaque, we only saw
a few number of particles, two in
subendothelial space and Most in
adventitia

24. Liver High dose
Day 1 3 5

25. Comparison of the Number of Iron
Particles in ApoE &Normal Mice
0
5
10
15
Atherosclerotic
Aorta
Average
number of iron
particles per
sample
P <0.001
Normal

26. Conclusion
 1- The study was stopped by the animal
committee, we therefore need to repeat it in
order completely evaluate the biodistribution
of Feridex over the time
 2- Iron particles are preferentially
accumulated in atherosclerotic lesions
 3- There is a good correlation seen between
macrophage staining and iron staining. A
quantitative assessment is to be reported in
the final study

27. -… Conlcusion
 4- As expected, we have seen Feridex
particles are trapped by RES mostly in liver
and spleen, some pulmonary macrophages,
and also lymphatic nodes.
 5- We plan to redo the study with other soon
to be available contrast media names
Combidex, Clariscan, MION, LCIO, AND our
own plaque specific USPIO in
collaboration with Dr Daniel Chan, Dr.
Chen Ho.

29. Spinal Cord

30. Spine