bacterial database

2. Corynebacterium
Species Distribution Disease Shape & Size Motility Capsule/Spores Staining
Reaction
Optimal
Temerature
O2
requirement
Resistance
Corynebacterium
pyogenes
Mucous
membranes
Summer
mastitis
Cocbacilli/bacilli
2 um
None None G +ve 37 C Killedat
60C
Corynebacterium
ovis
Caseous
lymphangitis
insheep
Filamentous
rods 1-3 um
None None G +ve 37 C
Facultative
anaerobe
Killedat
60C
Coreynebacterium
equi
Lymphangitis
inhorses
Pneumoniain
foals
Cocobacilli to
longrods or
clubshaped
None None G +ve 37 C
aerobe
Killedat
60C
Corynebacterium
renale
Pyelonehritis
cystitis
Rod shaped None None G +ve 37 C Killedat
60C
Corynebacterium
suis
Pyelonephritis
Cystitisinpigs
Rod shaped None None G +ve 37 C Killedat
60C
Corynebacterium
bovis
Bovine Milk Bovine
mastitis
Rod shaped None None G +ve 37 C Killedat
60C
Species Media Colonies
Corynebacteriumpyogenes Blood/serumagar Grey,1 mm diameter,zone of hemolysis
Corynebacteriumovis Nutrient/serumagar Nutrientagar,greyishandgranular
Serumagar, yellowcolonies
Coreynebacteriumequi Nutrientagar Glisteningmucoid,pale pinkcolonies
Corynebacteriumrenale Nutrient/Blood/Milkagar Bloodagar, moist,greywhite colonies
Milkagar, surroundedby a zone of hydrolysis
Corynebacteriumsuis Bloodagar
Corynebacteriumbovis Sheepbloodagar/10%serum+nutrientagar Sheepbloodagar,Small smoothcolonies

3. Pseudomonas
Species Distribution Disease Shape & Size Motility Capsule/Spores Staining
Reaction
Optimal
Temerature
O2
requirement
Resistance
Pseudomonas
aeroginosa
intestines Bovine
intestine,
urogenital
tract
infection,
enteritis,liver
abscesses,
peritonitis,
arthritis
Rod shaped
1.5x0.5 um
Yes None G -ve 37 C
Range 4-43C
Killedat
55C for 1
hr.
Pseudomonas
mallei
soil Glandersin
Humans&
Animals
Rod shaped
2-4x0.5 um
None None G -ve 37 C
aerobe
Moist heat
at 55C for 1
hr
Pseudomonas
pseudomallei
Human meliodiosis Rod shaped
2-4x05 um
Polar
flagella
None G –ve
bipolar
37 C Moist heat
at 55C for 1
hr
Species Media Colonies
Pseudomonasaeroginosa Nutrientagar Convex,glistening,spreadingedges,which
florese withflorescentcolorif floresing
bacteria( pyocyanin/florescin)
Pseudomonasmallei Glycerol agar,serumagar Small,yellowslimycoloniesonserumagar
Pseudomonaspseudomallei Glycerol agar,macConkey’sagar Earthy odor,corrugated,mucoid,pale colored
colonies

4. Mycobacterium
Species Distribution Disease Shape &
Size
Motility Capsule/Spores Staining
Reaction
Optimal
Temerature
O2
requirement
Resistance
Mycobacterium
tuberculosis
Human beings,
intestines/respiratory
tract
Tuberculosis
Intestine,
bones and
mostly
repiratory
forms
Rod shaped
1-4x0.2-0.3
um
None None Acidfast 37 C
Aerobe
Killedat
60C for 15
min.
Mycobacterium
bovis
Bovines Domestic
animals
tuberculosis,
excretedIn
milk
Rod shaped
1-4x0.2-0.3
um
None None Acidfast 37 C
Aerobe
Killedat
60C for 15
min.
Mycobacterium
avium
Avesspeciesand
AIDSpatientsHuman
MAC in AIDS
Patientsand
tuberculosis
inaves
species
Rod shaped
1-4x0.2-0.3
um
None None Acidfast 40 C range
42-43C
Aerobe
Killedat
60C for 15
min.
Mycobacterium
murium
Murine typesanimals Tuberculosis Rod shaped
1-4x0.2-0.3
um
None None Acidfast 37-38C
Aerobe
Killedat
60C for 15
min.
Mycobacterium
piscium
Coldblooded
especiallyfish
Tuberculosis Rod shaped
1-4x0.2-0.3
um
None None Acidfast 25C
Aerobe
Killedat
60C for 15
min.
Mycobacterium
paratuberculosis
Worldwide-Cattle,
Horse,sheepand pig
Enteritis Rod shaped
1-2x0.5 um
None Yes inaerobic
environment
G +ve,
Acidfast
38-39C Killedat
60C for 15
min.

5. Species Media Colonies
Mycobacteriumtuberculosis Mediacontainingegg,serumandpotato, Egg
dorsetmedium,Lowenstein-jensenmedium
(diferntial growth)
Produce yellow,yellowishpigmentoncultures
Mycobacteriumbovis Egg dorsetmedium,stonebrinksmedium
(differential growth)
Mycobacteriumavium Egg dorsetmedium,glycerine enhances
growth
Produce yellow,yellowishpigment oncultures
withslightpinkdiscoloration
Mycobacteriummurium Potato, eggdorsetmedium
Mycobacteriumpiscium Ordinarylabmediawithglycerine
Mycobacteriumparatuberculosis Mediacontainingkilledmycobacteriaortheir
extracts
Minute,greyish white,friable,irregular
colonies
Bacillus
Species Distribution Disease Shape &
Size
Motility Capsule/Spores Staining
Reaction
Optimal
Temerature
O2
requirement
Resistance
Bacillusanthracis Worldwide,cattle,
horse,sheepand
pigs.
Human beings, Soil-
borne
Anthrax Rod shaped
4-8x1-1.5
um
None Yes inaerobic
environment
G +ve
Spores
stainwith
methylene
blue
37 C
12-44 C
range
Killedat
60C for 15
min.
Sporesat
100C for 10
min.
Species Media Colonies
Bacillusanthracis Nutrien&Bloodagar Nutrientagar,rough,white andfrosted and
medusahead
Bloodagar-slighthemolysis

6. Clostridium
Species Distribution Disease Shape & Size Motility Capsule/Spores Staining
Reaction
Optimal
Temerature
O2
requirement
Resistance
Clostridiumtetani Worldwide
soil
saprophyte
Tetnus Rod shaped
2-4x0.5 um
peritrichous Drumstickspores G +ve 37 C (14-44C
range)
Anaerobe
Killedat
100C for 15
min.,120C
moistheat
20 minfor
spores
Clostridium
botuinum
Worldwide
soil
saprophyte
Botulism rods 4-
6x1um
Sluggishly
motile
None G +ve 35 C
anaerobe
Moist heat
100C for 5-
7 hours
Clostridium
oedematiens/noyi
Worldwide
soil
saprophyte
Black disease,
Gas gangrene,
Big Head
Rods5-8x1-
1.5 um
motile Oval,
central/subterminal
G +ve 37 C
anaerobe
Moist heat
120C for 10
min
Clostridium
septicum
Worldwide
soil
saprophyte
Braxysheep,
Black Quarter
cattle,sheep
Rod shaped
2-10x4um
motile -do- G +ve 37 C
anaerobe
Similarto
chauvoei
Clostridium
chauvoei
Worldwide
soil
saprophyte
Gas Gangrene,
blackQuarter
Rod shaped
3-8x0.5 um
motile -do- G +ve 37 C
anaerobe
Steamfor
40-50 min
Autoclave
Clostridium
welchii
Worldwide
soil
saprophyte
Gas gangrene-
Man
Enterotoxemia
insheepand
goat
Rod shaped
2-6x0.8-1.5
um
None -do- G +ve 37-47 C
anaerobe
Killedat
100C for 5
min
boiling,
Type A&C
100 C for1
hr

7. Species Media Colonies
Clostridiumtetani Blood/serumagar Grey,1 mm diameter,zone of hemolysis
Clostridiumbotuinum Nutrient/serumagar Nutrientagar,greyishandgranular
Serumagar, yellowcolonies
Clostridiumoedematiens/noyi Nutrientagar Glisteningmucoid,pale pinkcolonies
Clostridiumsepticum Nutrient/Blood/Milkagar Bloodagar, moist,greywhite colonies
Milkagar, surroundedbya zone of hydrolysis
Clostridiumchauvoei Bloodagar
Clostridiumwelchii Sheepbloodagar/10%serum+nutrientagar Sheepbloodagar,Small smoothcolonies