AUA 2019 Podium Presentation - Lower urinary tract ultrasound findings and increased oxidative stress in the bladder wall of men undergoing open prostatectomy
This study evaluated the link between preoperative lower urinary tract (LUT) ultrasound and oxidative stress (OS) markers in the bladder wall of men undergoing open prostate surgery
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AUA 2019 Podium Presentation - Lower urinary tract ultrasound findings and increased oxidative stress in the bladder wall of men undergoing open prostatectomy
1. PD61-11
Lower urinary tract ultrasound
findings and increased
oxidative stress in the bladder
wall of men undergoing open
prostatectomy
Márcio Averbeck, MD, MSc, PhD
marcioaverbeck@gmail.com
2. Lower urinary tract ultrasound findings
and increased oxidative stress in the
bladder wall of men undergoing open
prostatectomy
Averbeck MA1, De Lima NG2, Mo5a G1, Beltrao L1,
Abboud Filho N1, Rigo? C1, Dos Santos W1, Kitzberger S1,
Da Silva L1, Rhoden E1
1. Federal University of Health Sciences of Porto Alegre, Brazil
2. Hospital Nossa Senhora dos Navegantes, Torres, Brazil
6. Material and Methods
Exclusion criteria:
• Absence of voiding LUTS
• Previous pelvic surgery
• Previous pelvic radiaSon therapy
• Neurogenic lower urinary tract dysfuncSon
• Use of medicines with anScholinergic burden (e.g. anSmuscarinics, tricyclic
anSdepressants)
• Use of 5-alpha-reductase inhibitors
8. Material and Methods
PreoperaSve LUT ultrasound (US)
• Performed transabdominally
• Single trained researcher
• High frequency transducer (7.5 MHz) - Siemens Sonoline
G50® (Siemens AG, Munich, Germany)
marcioaverbeck@gmail.com
9. Material and Methods
US parameters:
• Prostate volume (PV)
• Intravesical prostaSc protrusion
(IPP)
• Bladder wall thickness (BWT)*
* BWT was defined by the distance between the mucosa and the advenSSa, both
with hyperechogenic characterisScs, and was measured by a mean of two sagi5al
measurements of the anterior bladder wall, with 250 mL of bladder filling.
Kuo et al. Neurourology and
Urodynamics DOI 10.1002/nau
IPP
10. Material and Methods
• A full-thickness fragment of the bladder wall
measuring 1.0 cm2 was obtained from the anterior
bladder wall during prostatectomy for
determinaSon of oxidaSve stress markers (liquid
nitrogen) and collagen deposiSon (formalin).
11. Material and Methods
COLLAGEN CONTENT ANALYSIS
• For each paraffin block, 5 cuts (3 to 5 micrometers) were made and stained
with 0.2% sirius red-picric acid soluSon and hematoxylin-eosin
• 25 random images (5 of each cut) were captured by a digital camera
(DP72®, Olympus, Tokyo, Japan), which was coupled to an opScal
microscope (BX51®, Olympus, Tokyo, Japan).
• Polarized light was used to intensify the normal birefringence of collagen
fibers.
12. Material and Methods
OxidaSve stress markers:
• Catalase (anSoxidant enzyme)
• SOD = superoxide dismutase
(anSoxidant enzyme)
• MDA = malondialdehyde (biomarker of
peroxidaSve damage to lipids)
marcioaverbeck@gmail.com
20. Conclusions
LUT US parameters, including increased bladder wall
thickness, prostate volume and post-void residual urine, were
associated with increased OS in the bladder wall of men with
LUTS undergoing open prostatectomy.
Identification of such factors may have clinical relevance, as
there might be a relationship between increased OS and
bladder dysfunction.
Future research should focus on non-invasive OS biomarkers.
