This document discusses the development of an LC-MS/MS method for the quantification of the biotherapeutic protein infliximab (Remicade) in rat plasma. Unique tryptic peptides from the light and heavy chains of infliximab were identified. The Skyline software was used to generate multiple reaction monitoring (MRM) transitions for the signature peptides and optimize collision energies. The optimized MRM method was then used to analyze plasma samples on a mass spectrometer to quantify infliximab.
This is Part 2 of a presentation on Genetic Toxicology that was given by Dr. David Kirkland to scientific staff at Health Canada in Nov. 2010. Part 1 is availabile here in ppt and as a webinar at the LinkedIn DABT CE group link
3-D QSAR studies on maslinic acid analogsNeha Dhiman
this is related with in silico studies of the compound obtained from plant and it was found as has anticancer property so can be used for the treatment of breast cancer. but still the synthesis procedure is remaining.
This is Part 2 of a presentation on Genetic Toxicology that was given by Dr. David Kirkland to scientific staff at Health Canada in Nov. 2010. Part 1 is availabile here in ppt and as a webinar at the LinkedIn DABT CE group link
3-D QSAR studies on maslinic acid analogsNeha Dhiman
this is related with in silico studies of the compound obtained from plant and it was found as has anticancer property so can be used for the treatment of breast cancer. but still the synthesis procedure is remaining.
This presentation helps you understand how you can setup a fast, easy and effective employee referral program using the wisestep.com platform. Use it to believe it !
ATENÇÃO LIDERANÇAS<<<
Link liberado para pré cadastro:
FAÇA JÁ SEU CADASTRO E SEJA PIONEIRO NESSE NEGÓCIO ESPETACULAR!!!!!!!
http://clubedelideres.net/?afiliado=fotografia
VEJAM AS VANTAGENS DE FAZER PARTE DO CDL
CLUBE DE LIDERES: Você obterá por só R$ 25,00.
• Sistema de Gerenciamento de Rede com direito de revenda e 100% de lucro!
• Ganhos rápidos de ciclos na matriz 2x2 (mesmo só com derramamentos)
• Residual mensal na matriz global 2x10 – meta da primeira fase R$ 6.963,00
• Premiações na matriz 2x7 conforme for atingindo os níveis sendo eles:
1º nível: página de captura
2º nível: auto responder
3º nível: site personalizado
4º nível: adesão na primeira empresa de mmn
5º nível: adesão na segunda empresa de mmn
6º nível: adesão na terceira empresa de mmn
7º nível: adesão na quarta empresa de mmn
• E muito mais novidades chegando por ai!
assista ao vídeo https://www.youtube.com/watch?v=ShXwyFNikVE
Seja um vencedor!
FAÇA JÁ SEU CADASTRO E ATIVE SUA CONTA
http://clubedelideres.net/?afiliado=fotografia
jrfotografia.desner@outlook.com
VAMOS QUE VAMOS PARA O SUCESSO!!!
Onze catalogus compacte schaarheftafels aan u gepresenteerd in het Duits, Intraboss is importeur voor de BeNeLux van Gruse schaarheftafels, U kunt bij ons terecht voor schaarheftafels op maat in elke prijsklasse en standaard uitvoeringen, onze schaarheftafels worden geleverd met CE en worden gebouwd volgens de EN 1570-1 norm
This presentation helps you understand how you can setup a fast, easy and effective employee referral program using the wisestep.com platform. Use it to believe it !
ATENÇÃO LIDERANÇAS<<<
Link liberado para pré cadastro:
FAÇA JÁ SEU CADASTRO E SEJA PIONEIRO NESSE NEGÓCIO ESPETACULAR!!!!!!!
http://clubedelideres.net/?afiliado=fotografia
VEJAM AS VANTAGENS DE FAZER PARTE DO CDL
CLUBE DE LIDERES: Você obterá por só R$ 25,00.
• Sistema de Gerenciamento de Rede com direito de revenda e 100% de lucro!
• Ganhos rápidos de ciclos na matriz 2x2 (mesmo só com derramamentos)
• Residual mensal na matriz global 2x10 – meta da primeira fase R$ 6.963,00
• Premiações na matriz 2x7 conforme for atingindo os níveis sendo eles:
1º nível: página de captura
2º nível: auto responder
3º nível: site personalizado
4º nível: adesão na primeira empresa de mmn
5º nível: adesão na segunda empresa de mmn
6º nível: adesão na terceira empresa de mmn
7º nível: adesão na quarta empresa de mmn
• E muito mais novidades chegando por ai!
assista ao vídeo https://www.youtube.com/watch?v=ShXwyFNikVE
Seja um vencedor!
FAÇA JÁ SEU CADASTRO E ATIVE SUA CONTA
http://clubedelideres.net/?afiliado=fotografia
jrfotografia.desner@outlook.com
VAMOS QUE VAMOS PARA O SUCESSO!!!
Onze catalogus compacte schaarheftafels aan u gepresenteerd in het Duits, Intraboss is importeur voor de BeNeLux van Gruse schaarheftafels, U kunt bij ons terecht voor schaarheftafels op maat in elke prijsklasse en standaard uitvoeringen, onze schaarheftafels worden geleverd met CE en worden gebouwd volgens de EN 1570-1 norm
Medicilon has strong capability in assay development and compound screening.Our screening capacity could be 200x384 well/week,or customize it per clients' needs.We work closely with partners such as Selleck,MCE and Tocris that provide compound libraries, and we have more than a library of 3000 compounds in-house.
https://www.medicilon.com/services/high-throughput-screening/
Large Molecule Bioanalysis: LC-MS or ELISA? A Case StudyQPS Holdings, LLC
Both ligand binding assays (LBA) and mass spectrometry
(MS) methods are widely used in routine bionalysis with expanded applications of MS to large molecules in
the years ahead. In this presentation, PK parameters in the rart for three monoclonal antibodies (mAbs) are compared as derived from plasma concentration data as measured by LBA or Immunocapture LC-MS/MS.
QSP is defined as an approach to translational medicine that combines computational and experimental methods to elucidate, validate, and apply new pharmacological concepts to the development an use of small molecule and biologic drugs.
RAS is one of the most frequently mutated oncogenes in human cancer. KRAS is the isoform most frequently mutated, which constitutes about 85% of RAS mutations. As the most frequently mutated RAS isoform, KRAS is intensively studied in the past years.
In the formulation of KRAS integrated research plan, Medicilon has in-depth communication with customers. The backbone of scientific research has combined the characteristics of each case with years of practical experience and technical accumulation, and carefully submitted high-quality experimental plans and results to customers. Medicilon provides KRAS-targeted drug discovery, CMC research (API + formulation), pharmacodynamics research, PK study, safety evaluation and other services.https://www.medicilon.com/platform/kras/
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...QIAGEN
The original microRNA quality control (miRQC) study provided an in-depth analysis of commercially available microRNA (miRNA) quantification platforms. Specifically, twelve different
microarray, real-time PCR and small RNA sequencing platforms were assessed for reproducibility, sensitivity, accuracy, specificity and concordance of differential expression using a variety of sample types. Overall, each platform exhibited specific strengths and weaknesses, leading to the
final suggestion that a platform should be chosen on the basis of the experimental setting and the specific research questions. With this suggestion in mind, and the fact that liquid miRNA biopsies are an area of intense interest, we sought to expand the original miRQC study. For our “miRQC extension,” we benchmarked the QIAGEN miScript® PCR System with and without preamplification, and included a specific focus on routinely used biofluids. Concurrently, we benchmarked the miScript PCR System against another SYBR® Green miRNA detection platform. Overall, QIAGEN miScript demonstrated strong reproducibility and accuracy as well as superior detection rate and sensitivity in biofluids. Collectively, QIAGEN miScript provides the leading solution for novel miRNA discoveries.
Analysis of pesticides in food using both LC- and GC-MS/MS, with data and description of Atmospheric Pressure GC, available on the same system as UPLC-MS/MS with rapid changeover.
Technical Guide to Qiagen PCR Arrays - Download the GuideQIAGEN
Total RNA discovery with RT2 and miScript PCR Arrays : Explore the RNA universe - Whatever your destination within the RNA universe, QIAGEN will help you get there. The miRNeasy kits deliver pure, high-quality total RNA from a broad range of samples. The RT2 and miScript PCR arrays are a complete solution both for focused analysis of gene and microRNA expression and for validation of microarray and RNA sequencing experiments. Together with the powerful analytics tools of GeneGlobe® and QIAGEN Ingenuity® Pathway Analysis, these products give you a smooth path from your sample to high-quality results.
Quantitative Analysis of Transporter Protein using TripleTOF® 6600 SystemSCIEX
Transport plays an important role in the absorption, distribution, and elimination of a variety of drugs.
In recent years, a large number of transporters, both efflux (ATP-binding cassette (ABC) family) and influx (solute carrier (SLC) family members) have been identified and well characterized in vitro.
However, the abundance of these transporters in the hepatocyte and cell lines as well as in the tissues such as intestine, liver, and kidney has not been accurately quantitated due to technical challenges.
This work aims to build a robust liquid chromatography-mass spectrometry (LC-MS) workflow on the SCIEX TripleTOF® 6600 platform to enable the quantitation of a variety of SLC and ABC drug transporters expressed in the hepatocyte and cell line plasma membranes.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.