1) Creative Biolabs offers a one-stop antibody-drug conjugate (ADC) development service including antibody screening, ADC assembly, in vitro analysis, and in vivo analysis. 2) Their antibody screening services include consulting on antigen selection, screening for antigen-specific and internalizing antibodies, and validating proof-of-concept using "Anti-Ab ADCs". 3) They select a suitable payload drug, couple it with a desired linker, and conjugate it to the selected antibody to assemble the ADC for analysis and pre-clinical evaluation before clinical applications.

1. One-stop ADC Development Service

2. INTRODUCTION
• Monoclonal antibody-based immunotherapies against cancer and other infectious diseases are
highly advantageous comparing to conventional therapeutic approaches due to their high specificity
and affinity towards well-defined targets.
• Antibody-drug conjugates (ADCs) inherit such superiorities and more remarkably, expand the
therapeutic window of the conjugated drugs (payloads), which are usually highly toxic and diverse in
their biochemical nature.

3. ADC mode of action. ADC molecules travel
to the tumor site via systematic circulation,
bind to tumor surface antigens, and enter
the tumor cells via receptor mediated
endocytosis (internalization). The payload is
released in the cytosol or lysosome to
eliminate the cancer cell by disrupting
important cellular pathways (microtubule
assembly, DNA transcription, mRNA
processing…).

4. One-stop ADC Development Service
A flow chart summarizing the design of an ADC. Correlations between different components in an
ADC intertwine, making the ADC design a challenging yet interesting process.

5. Antibody screening services

6. Antibody screening services
• At the starting point of an ADC development
project, Creative Biolabs offers antibody
screening services including the consultation in
antigen selection, antigen-specific antibody
screening, internalizing antibody selection, as
well as proof-of-concept feasibility validation
using “Anti-Ab ADCs”.
• A suitable payload drug will be selected and
coupled with a linker with desired releasing
mechanism in “DrugLnk” to assemble the
“chemical warhead” for an ADC, which is
subsequently conjugated with the desired
antibody using Antibody Design and
Conjugation services. Certain modification or
engineering of the selected antibody is also
carried out in this service module.

7. ADC in vitro Analysis

8. ADC in vitro Analysis
• After ADC assembly, its biochemical characteristics (purity, stability, drug-to-antibody
ratio…) and in vitro efficacy is adequately analyzed in ADC in vitro Analysis while the
ADC’s pre-clinical pharmacological parameters will be assayed in ADC in vivo Analysis
before clinical evaluation and applications.

9. Antibody-based Probes

10. Antibody-based Probes
• Lastly, Creative Biolabs is also an expert in the conjugation of various molecular
probes to antibodies to form Antibody-based Probes and we offer this service to
facilitate the requirements for the exponentially developing fields such as high-
resolution imagery and fast, accurate diagnostics.

11. THANK YOU