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(a) What are two ways in which the regulation of ColE1 plasmid replication is similar to the
regulation of tra operon expression in F plasmid? (b) What would happen to colE1 plasmid copy
number with a promoter mutation that decreases the expression of the Rop protein? (c) What
would happen to colE1 plasmid copy number with a promoter mutation that decreases the
expression of the RNA-II transcript? (d) What would happen to colEl plasmid copy number with
a promoter mutation that decreases the expression of the RNA-I transcript?

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