This document provides a summary of recent literature on allergy and immunology. It discusses several topics, including adherence to immunotherapy during financial crises, allergic reactions after immunization, and diagnosing gelatin allergy following vaccine reactions. The purpose is exclusively educational to provide updated knowledge for physicians, and does not replace clinical judgment. Suggestions to improve the content are welcome.
This document provides a summary of recent publications in allergy and immunology. It discusses the results of studies on the effects of acupuncture for allergic rhinitis, the lack of correlation between blood and airway eosinophil counts in children with severe asthma, and new insights into eosinophil-derived cytokines and their selective secretion. The purpose is to provide updated practical knowledge for allergy/immunology physicians.
2013 July - Pearls in Allergy and ImmunologyJuan Aldave
The document provides a summary of recent literature on allergy and immunology. It discusses several topics:
1) A consensus report that recommends the term "allergen immunotherapy" to describe therapy of allergic diseases with allergen-containing products.
2) Research on eosinophils and their role in allergic inflammation, including potential new treatment targets.
3) A position paper on desensitization for patients with delayed drug hypersensitivity reactions.
4) A study finding frequent sensitization to Candida albicans and profilins in adult eosinophilic esophagitis patients.
The purpose is to provide updated practical knowledge for physicians, though it does not replace clinical judgment
Pearls in Allergy and Immunology 2013 SeptemberJuan Aldave
This document provides a summary of recent literature on allergy and immunology. It discusses several topics:
- Thermal processing can reduce the allergenicity of some tree nuts but not others. Skin testing is useful for diagnosing proton pump inhibitor allergies.
- Atopic eczema is caused by skin barrier defects, innate immune dysregulation that promotes TH2 inflammation, and adaptive immune dysregulation from genetic and environmental factors.
- The document is intended for educational purposes for allergy/immunology physicians and does not replace their clinical judgement. Suggestions to improve the content should be sent to the author.
This document provides a summary of recent literature on allergic diseases from the journal Allergy in January 2013. It includes the following key points:
- A study found that uncontrolled asthma and nasal congestion are independent risk factors for insomnia.
- Studies showed that sublingual grass tablet immunotherapy had similar efficacy and safety in both mono- and polysensitized individuals with grass pollen allergy.
- A study found that higher maternal vitamin D levels during pregnancy were associated with increased risk of food allergy in children in the first 2 years of life.
- Mechanisms of peripheral tolerance to allergens were summarized, including the roles of regulatory T cells, dendritic cells, cytokines, and cell surface
Pearls in Allergy and Immunology - July 2014Juan Aldave
The purpose of the document is to provide educational material for Allergy/Immunology physicians. It summarizes several recent journal articles on topics related to allergy and immunology. Specifically, it discusses that allergic rhinitis is a risk factor for decreased traffic safety due to impaired cognitive and psychomotor functions. It also summarizes articles on differences in IgE sensitization patterns between atopic dermatitis and other hyper-IgE syndromes. Additionally, it discusses the efficacy of IgE-targeted versus empirical elimination diets for eosinophilic esophagitis.
Pearls in Allergy and Immunology, December 2013Juan Aldave
The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not intend to replace the clinical criteria of the physician.
- A study of 204 patients with delayed hypersensitivity to penicillins found that all patients tolerated drug challenges with carbapenems, suggesting an absence of cross-reactivity. Skin tests and graded challenges are still advised for patients needing carbapenems who experienced severe penicillin reactions.
- Anaphylaxis is an acute severe allergic reaction potentially fatal. Most common triggers are foods, drugs, insects. Factors influencing severity include pathogenic mechanism, allergen properties, dose, route of exposure, sensitization level.
Pearls in Allergy and Immunology October 2013Juan Aldave
This article discusses cofactors that can lower the threshold for anaphylaxis in sensitized individuals. Common cofactors include physical exercise, alcohol, infections, NSAIDs, and certain drugs. Mechanisms by which cofactors can induce anaphylaxis include increasing allergen absorption, lowering the activation threshold of mast cells and basophils, increasing leukotriene synthesis, and stimulating the immune system during infections. Understanding cofactors is important for managing anaphylaxis risk.
Pearls in Allergy and Immunology - June 2014Juan Aldave
The document provides an overview of recent literature on allergic diseases and immunology from the journal Allergy in June 2014. It summarizes several articles, including on the classification, diagnosis and treatment of angioedema, guidelines on the primary prevention of food allergy, a global survey on the classification and coding of hypersensitivity diseases, and gaps in evidence around factors that may influence the development of food allergies. The purpose is to provide updated practical knowledge for physicians in allergy and immunology.
This document provides a summary of recent publications in allergy and immunology. It discusses the results of studies on the effects of acupuncture for allergic rhinitis, the lack of correlation between blood and airway eosinophil counts in children with severe asthma, and new insights into eosinophil-derived cytokines and their selective secretion. The purpose is to provide updated practical knowledge for allergy/immunology physicians.
2013 July - Pearls in Allergy and ImmunologyJuan Aldave
The document provides a summary of recent literature on allergy and immunology. It discusses several topics:
1) A consensus report that recommends the term "allergen immunotherapy" to describe therapy of allergic diseases with allergen-containing products.
2) Research on eosinophils and their role in allergic inflammation, including potential new treatment targets.
3) A position paper on desensitization for patients with delayed drug hypersensitivity reactions.
4) A study finding frequent sensitization to Candida albicans and profilins in adult eosinophilic esophagitis patients.
The purpose is to provide updated practical knowledge for physicians, though it does not replace clinical judgment
Pearls in Allergy and Immunology 2013 SeptemberJuan Aldave
This document provides a summary of recent literature on allergy and immunology. It discusses several topics:
- Thermal processing can reduce the allergenicity of some tree nuts but not others. Skin testing is useful for diagnosing proton pump inhibitor allergies.
- Atopic eczema is caused by skin barrier defects, innate immune dysregulation that promotes TH2 inflammation, and adaptive immune dysregulation from genetic and environmental factors.
- The document is intended for educational purposes for allergy/immunology physicians and does not replace their clinical judgement. Suggestions to improve the content should be sent to the author.
This document provides a summary of recent literature on allergic diseases from the journal Allergy in January 2013. It includes the following key points:
- A study found that uncontrolled asthma and nasal congestion are independent risk factors for insomnia.
- Studies showed that sublingual grass tablet immunotherapy had similar efficacy and safety in both mono- and polysensitized individuals with grass pollen allergy.
- A study found that higher maternal vitamin D levels during pregnancy were associated with increased risk of food allergy in children in the first 2 years of life.
- Mechanisms of peripheral tolerance to allergens were summarized, including the roles of regulatory T cells, dendritic cells, cytokines, and cell surface
Pearls in Allergy and Immunology - July 2014Juan Aldave
The purpose of the document is to provide educational material for Allergy/Immunology physicians. It summarizes several recent journal articles on topics related to allergy and immunology. Specifically, it discusses that allergic rhinitis is a risk factor for decreased traffic safety due to impaired cognitive and psychomotor functions. It also summarizes articles on differences in IgE sensitization patterns between atopic dermatitis and other hyper-IgE syndromes. Additionally, it discusses the efficacy of IgE-targeted versus empirical elimination diets for eosinophilic esophagitis.
Pearls in Allergy and Immunology, December 2013Juan Aldave
The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not intend to replace the clinical criteria of the physician.
- A study of 204 patients with delayed hypersensitivity to penicillins found that all patients tolerated drug challenges with carbapenems, suggesting an absence of cross-reactivity. Skin tests and graded challenges are still advised for patients needing carbapenems who experienced severe penicillin reactions.
- Anaphylaxis is an acute severe allergic reaction potentially fatal. Most common triggers are foods, drugs, insects. Factors influencing severity include pathogenic mechanism, allergen properties, dose, route of exposure, sensitization level.
Pearls in Allergy and Immunology October 2013Juan Aldave
This article discusses cofactors that can lower the threshold for anaphylaxis in sensitized individuals. Common cofactors include physical exercise, alcohol, infections, NSAIDs, and certain drugs. Mechanisms by which cofactors can induce anaphylaxis include increasing allergen absorption, lowering the activation threshold of mast cells and basophils, increasing leukotriene synthesis, and stimulating the immune system during infections. Understanding cofactors is important for managing anaphylaxis risk.
Pearls in Allergy and Immunology - June 2014Juan Aldave
The document provides an overview of recent literature on allergic diseases and immunology from the journal Allergy in June 2014. It summarizes several articles, including on the classification, diagnosis and treatment of angioedema, guidelines on the primary prevention of food allergy, a global survey on the classification and coding of hypersensitivity diseases, and gaps in evidence around factors that may influence the development of food allergies. The purpose is to provide updated practical knowledge for physicians in allergy and immunology.
Pearls in Allergy and Immunology - May 2014Juan Aldave
This document provides an overview of recent literature on drug allergies and hypersensitivity reactions. It discusses the definition and classification of drug hypersensitivity reactions based on symptoms, timing and pathogenic mechanisms. Drug hypersensitivity reactions can be either immune-mediated (allergic) involving specific antibodies or T cells against the drug, or non-immune mediated involving non-allergic mechanisms like nonspecific mast cell activation. The document also discusses pathogenic mechanisms for different types of drug allergies classified from type I to type IV, as well as mechanisms of immune activation against drugs like the hapten concept.
Pears in Allergy and Immunology, April 2014Juan Aldave
The document provides an educational summary of recent articles in allergy and immunology from April 2014. It includes summaries of articles on:
1) A position paper on work-related chronic cough that describes its causes, diagnosis, and management depending on etiology. Common causes include work-related asthma, rhinosinusitis, and COPD.
2) A review on the roles of histamine in the gut, including its pro-inflammatory and anti-inflammatory effects depending on receptor activation, and metabolism by diamine oxidase and histamine-N-methyltransferase.
3) A potential novel mechanism of allergen-specific desensitization involving IgG-mediated down-regulation of IgE bound to mast cells
The document provides an educational summary of recent literature on allergy and immunology for physicians. It includes brief summaries of multiple journal articles on topics like drug allergy diagnosis, anaphylaxis, asthma, food allergy, primary immunodeficiencies, and more. The purpose is stated as being exclusively educational to provide practical knowledge for physicians, while not replacing clinical judgment. Contact information is provided for the author in case of questions.
Pearls in Allergy and Immunology, March 2014Juan Aldave
The document summarizes a systematic review on the acute and long-term management of food allergy. Key points include:
- Food allergy can be IgE-mediated, non-IgE mediated, or both IgE- and cell-mediated. The most common allergenic foods are milk, egg, peanut, tree nuts, wheat, soy, and seafood.
- Diagnosis involves skin prick tests, in vitro IgE tests, and food challenges. Treatment focuses on avoidance, epinephrine autoinjectors, and monitoring for tolerance development.
- The review found weak evidence that antihistamines may help non-life threatening reactions and mast cell stabilizers may reduce symptoms. Extensively hydro
Pearls in Allergy and Immunology - February 2014 Juan Aldave
The document summarizes recent literature on allergic diseases from the journal Allergy and Annals of Allergy, Asthma and Immunology. It discusses topics such as atopic dermatitis, the atopic march, food allergy epidemiology and diagnosis, asthma phenotypes, new therapies for atopic dermatitis and allergic asthma, and the role of filaggrin mutations and phospholipids in omalizumab treatment efficacy for atopic dermatitis. The purpose is to provide updated knowledge for allergy/immunology physicians, though it does not replace clinical judgment. Any corrections or suggestions should be sent directly to the author.
Pearls in Allergy and Immunology - August 2014Juan Aldave
The document summarizes recent guidelines on anaphylaxis from the European Academy of Allergy and Clinical Immunology. It discusses:
1) The definition, prevalence, mechanisms, common triggers, factors influencing severity, diagnosis and treatment of anaphylaxis.
2) The mechanisms of action of epinephrine and its role as first-line treatment.
3) Common cofactors ("augmentation factors") that can lower the threshold for anaphylaxis, including physical exercise, alcohol, infections, NSAIDs and certain drugs.
The document provides an educational summary of recent literature on allergies and clinical immunology. It includes summaries of several articles: 1) "Basophils Unlimited" describes a new method for generating large quantities of mouse basophils for research; 2) "Dog Saliva" shows that dog saliva contains more diverse allergenic proteins than dander and may improve diagnostics; 3) An EAACI position paper analyzes the quality and standardization of skin prick testing solutions for occupational allergies. The summary also briefly outlines additional articles on basophil functions, the impact of intranasal corticosteroids on asthma outcomes in patients with allergic rhinitis, and low-dose aspirin desensitization
2013 August - Pearls in Allergy and ImmunologyJuan Aldave
This summary discusses two articles from the Annals of Allergy, Asthma & Immunology. The first article reports a case of relapsing polychondritis, an immune-mediated cartilage inflammation, in a patient who also had hypogammaglobulinemia of unknown origin. The patient's polychondritis significantly improved with corticosteroids and methotrexate. The second article reviews advances in the diagnosis and management of insect sting allergy, noting epidemiology, risk stratification of patients, diagnostic tests and their limitations, and novel diagnostic approaches such as basophil activation testing.
Pearls in Allergy and Immunology, November 2013Juan Aldave
- The document summarizes recent articles from Allergy and Annals of Allergy, Asthma & Immunology journals. It discusses topics such as exercise-induced bronchoconstriction in athletes, effectiveness of low-dose aspirin for treating aspirin-exacerbated respiratory disease, and diversity of allergens in dog saliva. The author is Juan Carlos Aldave Becerra, an allergy/immunology physician providing this educational summary for other physicians. Any feedback or questions should be directed to the author by email.
The document discusses vaccine hypersensitivity and provides the following information:
1. It outlines the evolution of immunization programs from the pre-vaccine era to modern times and discusses the relationship between vaccine usage and adverse events.
2. It reviews the epidemiology of immediate hypersensitivity reactions to vaccines in the US and Australia, finding reporting rates of 10 per 100,000 doses in the US and incidence of potential IgE-mediated reactions of 5.4 per 100,000 doses in Australia.
3. It examines allergic reactions to specific vaccine constituents like gelatin and egg, noting the need to consider alternative vaccines or precautions in individuals with a history of allergy to these ingredients.
Alpha-Gal Syndrome, also known as red meat allergy, is caused by IgE antibodies against the carbohydrate galactose-alpha-1,3-galactose (α-Gal) found in most mammals except primates. Tick bites transmit α-Gal to humans, causing an immune response. Reactions to red meat occur 2-6 hours after consumption due to the delayed absorption of α-Gal from glycoproteins and glycolipids. Management involves avoiding red meat, organs, and secondary exposures. The syndrome is increasingly recognized globally where ticks that transmit α-Gal are present.
FPIAP is a non-IgE mediated gastrointestinal food allergy that is the mildest form. It is characterized by the presence of blood and sometimes mucus in the stools of otherwise healthy infants. It affects infants under 6 months of age, with symptoms appearing between 2-8 weeks of life. The most common triggering foods are cow's milk protein in breastfed infants and soy, cow's milk protein, and extensively hydrolyzed formulas in formula-fed infants. The diagnosis is based on clinical history and resolution of symptoms with dietary elimination of trigger foods. Prognosis is excellent, with tolerance developing in most children by 1-3 years of age.
1. The document summarizes various studies on prevention of allergy through modulation of the gut microbiota from early life.
2. Key areas of focus include the gut microbiota of pregnant women and its impact on the infant, early introduction of foods like peanuts and eggs to induce tolerance, use of probiotics and breastfeeding to influence the infant gut microbiota, and effects of a farm environment and dietary fibers on allergy risk.
3. Studies found that probiotics started prenatally and combined with breastfeeding were most effective in preventing eczema, and that early introduction of peanuts and eggs reduced the risk of developing an allergy to those foods.
This document discusses wheat allergy and related topics. It begins by classifying wheat taxonomically and describing its major protein components, many of which are allergens. It then discusses the epidemiology and clinical manifestations of wheat allergy, including immediate IgE-mediated reactions, allergic contact urticaria, baker's asthma, food-dependent exercise-induced anaphylaxis, associations with atopic dermatitis, and eosinophilic gastrointestinal disorders. Mechanisms of allergenic cross-reactivity between wheat and other cereals as well as grasses are also reviewed.
This educational material from Juan Carlos Aldave Becerra aims to provide updated knowledge for allergy/immunology physicians. It discusses recent studies on various topics: the potential relationship between allergic rhinitis and erectile dysfunction; the role of extracellular DNA traps in allergic, infectious and autoimmune diseases; and the identification of galactose-α-1,3-galactose in ticks which may be related to red meat allergy. It also reviews several other recent articles on topics like food allergy, drug allergy, asthma treatments and immunodeficiencies. The author welcomes any feedback to improve the educational value.
Red meat allergy, also known as alpha-gal allergy, is caused by IgE antibodies to the carbohydrate epitope galactose-alpha-1,3-galactose (alpha-gal) found in mammalian meats. Certain tick bites, such as from the lone star tick, are associated with the production of these IgE antibodies. Reactions to red meat following tick bites are often delayed 3-6 hours after ingestion. The discovery of the alpha-gal allergy has provided insights into regional differences in food allergies and advanced understanding of how environmental exposures can induce novel food allergies.
1) A study of 651 children with pollen-related allergic rhinitis found that component-resolved diagnosis identified a lack of IgE antibodies to major allergens in a significant percentage of patients with skin prick test reactivity, calling into question the appropriateness of immunotherapy for some patients.
2) Without component-resolved diagnosis, immunotherapy would have been prescribed inappropriately in 37% of cases based on skin prick test alone.
3) Component-resolved diagnosis led European and American allergists to change their immunotherapy prescription decisions in 42-48% of cases compared to skin prick testing alone.
Pearls in Allergy and Immunology, January 2014Juan Aldave
The purpose of this educational material is to provide updated knowledge for Allergy/Immunology Physicians. It summarizes recent articles from peer-reviewed journals. If there are any corrections needed, they should be sent directly to the authors by email.
The document summarizes several articles from recent issues of peer-reviewed journals, including:
1) A case report of anaphylaxis induced by streptomycin skin testing, highlighting the risks of systemic reactions from skin testing.
2) An overview of aspirin-exacerbated respiratory disease and approaches to aspirin desensitization, which can effectively improve asthma and sinusitis outcomes.
3) A discussion of the low adherence rates to asthma
Pearls in Allergy and Immunology - May 2014Juan Aldave
This document provides an overview of recent literature on drug allergies and hypersensitivity reactions. It discusses the definition and classification of drug hypersensitivity reactions based on symptoms, timing and pathogenic mechanisms. Drug hypersensitivity reactions can be either immune-mediated (allergic) involving specific antibodies or T cells against the drug, or non-immune mediated involving non-allergic mechanisms like nonspecific mast cell activation. The document also discusses pathogenic mechanisms for different types of drug allergies classified from type I to type IV, as well as mechanisms of immune activation against drugs like the hapten concept.
Pears in Allergy and Immunology, April 2014Juan Aldave
The document provides an educational summary of recent articles in allergy and immunology from April 2014. It includes summaries of articles on:
1) A position paper on work-related chronic cough that describes its causes, diagnosis, and management depending on etiology. Common causes include work-related asthma, rhinosinusitis, and COPD.
2) A review on the roles of histamine in the gut, including its pro-inflammatory and anti-inflammatory effects depending on receptor activation, and metabolism by diamine oxidase and histamine-N-methyltransferase.
3) A potential novel mechanism of allergen-specific desensitization involving IgG-mediated down-regulation of IgE bound to mast cells
The document provides an educational summary of recent literature on allergy and immunology for physicians. It includes brief summaries of multiple journal articles on topics like drug allergy diagnosis, anaphylaxis, asthma, food allergy, primary immunodeficiencies, and more. The purpose is stated as being exclusively educational to provide practical knowledge for physicians, while not replacing clinical judgment. Contact information is provided for the author in case of questions.
Pearls in Allergy and Immunology, March 2014Juan Aldave
The document summarizes a systematic review on the acute and long-term management of food allergy. Key points include:
- Food allergy can be IgE-mediated, non-IgE mediated, or both IgE- and cell-mediated. The most common allergenic foods are milk, egg, peanut, tree nuts, wheat, soy, and seafood.
- Diagnosis involves skin prick tests, in vitro IgE tests, and food challenges. Treatment focuses on avoidance, epinephrine autoinjectors, and monitoring for tolerance development.
- The review found weak evidence that antihistamines may help non-life threatening reactions and mast cell stabilizers may reduce symptoms. Extensively hydro
Pearls in Allergy and Immunology - February 2014 Juan Aldave
The document summarizes recent literature on allergic diseases from the journal Allergy and Annals of Allergy, Asthma and Immunology. It discusses topics such as atopic dermatitis, the atopic march, food allergy epidemiology and diagnosis, asthma phenotypes, new therapies for atopic dermatitis and allergic asthma, and the role of filaggrin mutations and phospholipids in omalizumab treatment efficacy for atopic dermatitis. The purpose is to provide updated knowledge for allergy/immunology physicians, though it does not replace clinical judgment. Any corrections or suggestions should be sent directly to the author.
Pearls in Allergy and Immunology - August 2014Juan Aldave
The document summarizes recent guidelines on anaphylaxis from the European Academy of Allergy and Clinical Immunology. It discusses:
1) The definition, prevalence, mechanisms, common triggers, factors influencing severity, diagnosis and treatment of anaphylaxis.
2) The mechanisms of action of epinephrine and its role as first-line treatment.
3) Common cofactors ("augmentation factors") that can lower the threshold for anaphylaxis, including physical exercise, alcohol, infections, NSAIDs and certain drugs.
The document provides an educational summary of recent literature on allergies and clinical immunology. It includes summaries of several articles: 1) "Basophils Unlimited" describes a new method for generating large quantities of mouse basophils for research; 2) "Dog Saliva" shows that dog saliva contains more diverse allergenic proteins than dander and may improve diagnostics; 3) An EAACI position paper analyzes the quality and standardization of skin prick testing solutions for occupational allergies. The summary also briefly outlines additional articles on basophil functions, the impact of intranasal corticosteroids on asthma outcomes in patients with allergic rhinitis, and low-dose aspirin desensitization
2013 August - Pearls in Allergy and ImmunologyJuan Aldave
This summary discusses two articles from the Annals of Allergy, Asthma & Immunology. The first article reports a case of relapsing polychondritis, an immune-mediated cartilage inflammation, in a patient who also had hypogammaglobulinemia of unknown origin. The patient's polychondritis significantly improved with corticosteroids and methotrexate. The second article reviews advances in the diagnosis and management of insect sting allergy, noting epidemiology, risk stratification of patients, diagnostic tests and their limitations, and novel diagnostic approaches such as basophil activation testing.
Pearls in Allergy and Immunology, November 2013Juan Aldave
- The document summarizes recent articles from Allergy and Annals of Allergy, Asthma & Immunology journals. It discusses topics such as exercise-induced bronchoconstriction in athletes, effectiveness of low-dose aspirin for treating aspirin-exacerbated respiratory disease, and diversity of allergens in dog saliva. The author is Juan Carlos Aldave Becerra, an allergy/immunology physician providing this educational summary for other physicians. Any feedback or questions should be directed to the author by email.
The document discusses vaccine hypersensitivity and provides the following information:
1. It outlines the evolution of immunization programs from the pre-vaccine era to modern times and discusses the relationship between vaccine usage and adverse events.
2. It reviews the epidemiology of immediate hypersensitivity reactions to vaccines in the US and Australia, finding reporting rates of 10 per 100,000 doses in the US and incidence of potential IgE-mediated reactions of 5.4 per 100,000 doses in Australia.
3. It examines allergic reactions to specific vaccine constituents like gelatin and egg, noting the need to consider alternative vaccines or precautions in individuals with a history of allergy to these ingredients.
Alpha-Gal Syndrome, also known as red meat allergy, is caused by IgE antibodies against the carbohydrate galactose-alpha-1,3-galactose (α-Gal) found in most mammals except primates. Tick bites transmit α-Gal to humans, causing an immune response. Reactions to red meat occur 2-6 hours after consumption due to the delayed absorption of α-Gal from glycoproteins and glycolipids. Management involves avoiding red meat, organs, and secondary exposures. The syndrome is increasingly recognized globally where ticks that transmit α-Gal are present.
FPIAP is a non-IgE mediated gastrointestinal food allergy that is the mildest form. It is characterized by the presence of blood and sometimes mucus in the stools of otherwise healthy infants. It affects infants under 6 months of age, with symptoms appearing between 2-8 weeks of life. The most common triggering foods are cow's milk protein in breastfed infants and soy, cow's milk protein, and extensively hydrolyzed formulas in formula-fed infants. The diagnosis is based on clinical history and resolution of symptoms with dietary elimination of trigger foods. Prognosis is excellent, with tolerance developing in most children by 1-3 years of age.
1. The document summarizes various studies on prevention of allergy through modulation of the gut microbiota from early life.
2. Key areas of focus include the gut microbiota of pregnant women and its impact on the infant, early introduction of foods like peanuts and eggs to induce tolerance, use of probiotics and breastfeeding to influence the infant gut microbiota, and effects of a farm environment and dietary fibers on allergy risk.
3. Studies found that probiotics started prenatally and combined with breastfeeding were most effective in preventing eczema, and that early introduction of peanuts and eggs reduced the risk of developing an allergy to those foods.
This document discusses wheat allergy and related topics. It begins by classifying wheat taxonomically and describing its major protein components, many of which are allergens. It then discusses the epidemiology and clinical manifestations of wheat allergy, including immediate IgE-mediated reactions, allergic contact urticaria, baker's asthma, food-dependent exercise-induced anaphylaxis, associations with atopic dermatitis, and eosinophilic gastrointestinal disorders. Mechanisms of allergenic cross-reactivity between wheat and other cereals as well as grasses are also reviewed.
This educational material from Juan Carlos Aldave Becerra aims to provide updated knowledge for allergy/immunology physicians. It discusses recent studies on various topics: the potential relationship between allergic rhinitis and erectile dysfunction; the role of extracellular DNA traps in allergic, infectious and autoimmune diseases; and the identification of galactose-α-1,3-galactose in ticks which may be related to red meat allergy. It also reviews several other recent articles on topics like food allergy, drug allergy, asthma treatments and immunodeficiencies. The author welcomes any feedback to improve the educational value.
Red meat allergy, also known as alpha-gal allergy, is caused by IgE antibodies to the carbohydrate epitope galactose-alpha-1,3-galactose (alpha-gal) found in mammalian meats. Certain tick bites, such as from the lone star tick, are associated with the production of these IgE antibodies. Reactions to red meat following tick bites are often delayed 3-6 hours after ingestion. The discovery of the alpha-gal allergy has provided insights into regional differences in food allergies and advanced understanding of how environmental exposures can induce novel food allergies.
1) A study of 651 children with pollen-related allergic rhinitis found that component-resolved diagnosis identified a lack of IgE antibodies to major allergens in a significant percentage of patients with skin prick test reactivity, calling into question the appropriateness of immunotherapy for some patients.
2) Without component-resolved diagnosis, immunotherapy would have been prescribed inappropriately in 37% of cases based on skin prick test alone.
3) Component-resolved diagnosis led European and American allergists to change their immunotherapy prescription decisions in 42-48% of cases compared to skin prick testing alone.
Pearls in Allergy and Immunology, January 2014Juan Aldave
The purpose of this educational material is to provide updated knowledge for Allergy/Immunology Physicians. It summarizes recent articles from peer-reviewed journals. If there are any corrections needed, they should be sent directly to the authors by email.
The document summarizes several articles from recent issues of peer-reviewed journals, including:
1) A case report of anaphylaxis induced by streptomycin skin testing, highlighting the risks of systemic reactions from skin testing.
2) An overview of aspirin-exacerbated respiratory disease and approaches to aspirin desensitization, which can effectively improve asthma and sinusitis outcomes.
3) A discussion of the low adherence rates to asthma
Food allergy has been long recognized and well documented. Other adverse reactions to foods first referred to as “toxic idiopathies” by John Freeman, co inventor of immunotherapy, at the early part of the 1900s can be mediated by and have their impact on the nervous and endocrine systems. It can also be mediated by pharmacologic mechanisms and can also affect any part of the body. There’s a great clinical need to accurately identify triggers of adverse reactivity as they have now been linked with even the most serious of modern maladies and diseases. In fact, inflammation is the hallmark of metabolic syndrome. Given the multitude of pathogenic mechanisms underlying adverse reactions to foods and other environmental exposures it is necessary that a utilizable and cost effective technology be understood so that its application be utilized under the appropriate circumstances.
KEY LEARNING POINTS
• The natural ability of certain foods to initiate an inflammatory response and induce metabolic disruptions and counterbalancing mechanisms to prevent that
• How foods can trigger “danger signals” for the immune system
Pharmacologic vs. immunologic reactions to foods
• Is there a common final pathway of all these mechanisms that can reliably indicate triggers of clinical pathology?
• Cellular testing vs. serologic testing: The advantages of cellular testing
- Ahmed M Ebrahim, an 8-year-old boy, presents with cough, wheezing, dyspnea and has a history of asthma and allergic rhinitis since age 3. He has recurrent swelling behind the ears diagnosed as parotid.
- Examination finds depressed maxillary bones, atrophied nares, oral breathing, and vesicular breathing with rhonchi. Tests show normal CBC.
- The patient likely has Selective IgA Deficiency based on his clinical history of recurrent infections, asthma, and parotid swelling. This is the most common primary immune deficiency where patients have low or absent IgA levels.
This document discusses approaches to preventing allergic diseases in children. It mentions that allergic diseases have increased rapidly in recent decades likely due to environmental and lifestyle changes. Early life exposure to allergens and microbes may influence the development of allergic immune responses. The document discusses genetic and environmental risk factors for atopic dermatitis and reviews evidence on the role of various allergens like food, aeroallergens and bacteria. It proposes that primary prevention aims to prevent sensitization to allergens, secondary prevention prevents disease progression after sensitization and tertiary prevention reduces symptoms after disease onset through allergen avoidance and treatment.
This document summarizes a study on the relationship between atopic dermatitis and food allergy. It notes that while some studies have found a link between food allergies exacerbating atopic dermatitis symptoms, there is disagreement between dermatologists and allergologists on this relationship. The study performed a literature review and found many more reports on the topic in allergy journals compared to dermatology journals. It concludes that food elimination diets may be appropriate for some refractory atopic dermatitis patients, especially if testing finds clinical relevance between symptoms and specific foods, but their use requires careful implementation and follow-up.
Overlap between allergy and immunedeficiency originallllllFawzia Abo-Ali
Fawzia Abo Ali discusses the relationship between primary immunodeficiencies (PIDs) and allergies. She notes that allergies are sometimes an early warning sign of an underlying PID. PIDs like selective IgA deficiency, hyper IgE syndrome, common variable immunodeficiency, and severe combined immunodeficiency have been associated with higher rates of allergies like asthma and atopic dermatitis. A bias toward TH2 immune responses can also occur in PIDs like CVID. She stresses that PIDs should be considered in patients with severe, treatment-resistant, or infection-prone allergies. Early immunoglobulin testing is important to diagnose PIDs before organ
This document discusses sublingual immunotherapy (SLIT) for food allergies. It begins by defining SLIT and comparing it to subcutaneous immunotherapy (SCIT), noting that SLIT is a non-injection route that may help increase compliance. The mechanism of SLIT is described, including how the oral mucosa has immune-privileged cells that can induce tolerance. Studies on using SLIT for peanut allergy and milk allergy are summarized, outlining their methods, results, and findings regarding increased reaction thresholds, decreased immune markers, and minimal side effects.
Probiotic administration in early life, atopy, and asthma, a meta analysis of...Ariyanto Harsono
The document summarizes a meta-analysis of clinical trials on the effects of probiotic supplementation in children. The analysis found that prenatal and early-life probiotic administration reduced the risk of atopic sensitization and decreased total immunoglobulin E levels. However, probiotic administration may not reduce the risk of asthma or wheezing. The effects also depended on the probiotic strain used and the duration of follow-up. While probiotics seemed to reduce infant eczema, the evidence was heterogeneous and did not find an effect on confirmed atopic eczema. Overall, the review concluded there was insufficient evidence that probiotics prevent allergic disease or food hypersensitivity in infants.
Health economics perspective in allergy prevention in childrenAriyanto Harsono
This document discusses the economics of allergy prevention in children. It notes that the prevalence of allergic diseases is increasing, impacting both individuals and society through costs. Primary prevention strategies aim to recognize high-risk infants. Research shows exclusively breastfeeding for the first four months and introducing hydrolyzed formula for high-risk infants not breastfed can help prevent allergic diseases. Economic analyses from the perspectives of healthcare systems, families, and societies find hydrolyzed formulas, though costlier initially, reduce long-term costs through fewer cases of allergic disease compared to standard formulas. Partially hydrolyzed formulas are found to be cost-effective options for prevention compared to extensively hydrolyzed or soy formulas.
Secondary immunodeficiencies are caused by extrinsic factors that impair an otherwise normal immune system. They are far more common than primary immunodeficiencies and can manifest as increased susceptibility to common infections, unusual complications of infections, or opportunistic infections. Examples of conditions that can cause secondary immunodeficiency include HIV/AIDS, malnutrition, diabetes, renal failure, cirrhosis, extremes of age, Down syndrome, and metabolic diseases.
Src jbbr-21-125 Dr. ihsan edan abdulkareem alsaimary PROFESSOR IN MEDICAL M...dr.Ihsan alsaimary
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
This document summarizes research on vaccination in children with chronic diseases. It discusses issues with influenza and pneumococcal vaccinations in high-risk groups. It reviews studies examining the immunogenicity, safety and efficacy of influenza and pneumococcal vaccines in various chronic conditions like asthma, cancer, and immunosuppression. It also discusses gaps in knowledge around the impact of influenza in different at-risk groups and the need for more data on vaccine immunogenicity, safety and efficacy in each high-risk population. The document emphasizes the importance of recommending influenza vaccination for children with chronic diseases and implementing strategies to increase vaccination coverage.
This document discusses the historical perspective, epidemiology, pathogenesis, etiology, and immune responses related to allergic bronchopulmonary aspergillosis (ABPA). It notes that ABPA was first described in 1952 and involves an allergic response to the fungus Aspergillus, which commonly involves the lungs. The document outlines the involvement of innate immune responses like TLRs and acquired responses like specific antibodies and T cells. It also examines factors like genetic variants that can influence susceptibility.
Explore the cell's role in mediating adverse reactions 7 c09Paul Thiessen
This document discusses the role of neutrophils and macrophages in mediating various physiological and pathological processes. It summarizes several scientific studies that found:
1) Neutrophils can be recruited by substances like gliadin and mediate local inflammatory responses in tissues like the intestine.
2) Neutrophils and macrophages produce reactive molecules that can damage cells and tissues, and their activation levels correlate with conditions like infertility and acute coronary syndrome.
3) Chronic activation of the innate immune system by these cells may underlie metabolic syndrome by stimulating inflammation and hormonal changes.
4) Oxidative stress can increase blood levels of modified lipids implicated in atherosclerosis, coinciding with increased neutrophil counts.
Alergia a los almentos mediadas por anticuerpos ig g asociadas con la migraña...MEDIAGNOSTIC
This document discusses a study investigating the presence of IgG antibodies to foods in patients with migraine refractory to traditional treatment. The study found:
1) Statistical significant differences in the number of positive IgG food allergens between patients with migraine (56 patients) and a control group without migraine.
2) Elimination diets successfully controlled migraines in patients, without the need for medications.
3) According to the results, serum IgG antibodies to common foods should be investigated in patients with migraine.
This document discusses maternal probiotic supplementation to reduce infant eczema. It finds that probiotic supplementation during pregnancy and breastfeeding is associated with decreased prevalence of eczema in infants. Certain probiotic strains, such as Bifidobacterium bifidum, Bifidobacterium lactis, and Lactobacillus acidophilus, taken for longer durations (4-6 months before and after birth) can reduce infant eczema incidence by modulating the immune system and reducing inflammatory responses.
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2013 june Pearls in Allergy and Clinical Immunology
1. June 2013
General considerations:
• The purpose of this educational material is exclusively
educational, to provide practical updated knowledge
for Allergy/Immunology Physicians.
• The content of this educational material does not
intend to replace the clinical criteria of the physician.
• If there is any correction or suggestion to improve the
quality of this educational material, it should be done
directly to the author by e-mail.
• If there is any question or doubt about the content of
this educational material, it should be done directly to
the author by e-mail.
Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Hospital Nacional Edgardo Rebagliati Martins, Lima-Peru
jucapul_84@hotmail.com
Juan Félix Aldave Pita, MD
Medical Director
Luke Society International, Trujillo-Peru
2. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
June 2013 – content:
• ADHERENCE TO IMMUNOTHERAPY IN TIMES OF FINANCIAL CRISIS (González-de-Olano D, Álvarez-
Twose I. Ann Allergy Asthma Immunol 2013; 110: 466–468).
• ALLERGIC REACTIONS AFTER IMMUNIZATION (Kelso GM. Ann Allergy Asthma Immunol 2013; 110: 397–
401).
• DISCORDANCE BETWEEN AEROALLERGEN SPECIFIC SERUM IGE AND SKIN TESTING IN CHILDREN
YOUNGER THAN 4 YEARS (de Vos G, Nazari R, Ferastraoaru D, Parikh P, Geliebter R, Pichardo Y, Wiznia
A, Rosenstreich D. Ann Allergy Asthma Immunol 2013; 110: 438–443).
• EFFICACY OF OMALIZUMAB IN ASTHMATIC PATIENTS WITH IGE LEVELS ABOVE 700 IU/ML: A
RETROSPECTIVE STUDY (Maselli DJ, Singh H, Diaz J, Peters JI. Ann Allergy Asthma Immunol 2013; 110:
457–461).
• OXYTOCIN: A LIKELY UNDERESTIMATED RISK FOR ANAPHYLACTIC REACTIONS IN DELIVERING
WOMEN SENSITIZED TO LATEX (Liccardi G, Bilò MB, Mauro C, Salzillo A, Piccolo A, D’Amato M, D’Amato
G. Ann Allergy Asthma Immunol 2013; 110: 465–466).
• PEDIATRIC SUBLINGUAL IMMUNOTHERAPY EFFICACY: EVIDENCE ANALYSIS, 2009-2012 (Larenas-
Linnemann D, Blaiss M, Van Bever HP, Compalati E, Baena-Cagnani CE. Ann Allergy Asthma Immunol 2013;
110: 402–415).
• AN ALTERNATIVE EXPLANATION FOR THE INVERSE RELATIONSHIP BETWEEN ATOPY AND
MYOCARDIAL INFARCTION (Varner A. J Allergy Clin Immunol 2013; 131: 1715).
• APPROVED AND UNAPPROVED USE OF IMMUNOGLOBULINS IN ICELAND (Ólafsdóttir BE, Porsteinsson
DP, Einarsdóttir R, Ludvíksson BR, Gröndal G, Erlendsson K, Haraldsson A. J Allergy Clin Immunol 2013; 131:
1703-1705).
• EFFECT OF BARRIER MICROBES ON ORGAN-BASED INFLAMMATION (Garn H, Neves JF, Blumberg RS,
Renz H. J Allergy Clin Immunol 2013; 131: 1465-1478).
• ENDOTYPES AND PHENOTYPES OF CHRONIC RHINOSINUSITIS: A PRACTALL DOCUMENT OF THE
EUROPEAN ACADEMY OF ALLERGY AND CLINICAL IMMUNOLOGY AND THE AMERICAN ACADEMY
OF ALLERGY, ASTHMA & IMMUNOLOGY (Akdis CA, Bachert C, Cingi C, Dykewicz MS, Hellings PW,
Naclerio RM, Schleimer RP, Ledford D. J Allergy Clin Immunol 2013; 131: 1479-1490).
• EXPANDING THE PARADIGM OF EOSINOPHILIC ESOPHAGITIS: MAST CELLS AND IL-9 (Wang YH,
Hogan SP, Fulkerson PC, Abonia JP, Rothenberg ME. J Allergy Clin Immunol 2013; 131: 1583-1585).
• GAIN-OF-FUNCTION STAT1 MUTATIONS ARE ASSOCIATED WITH PD-L1 OVEREXPRESSION AND A
DEFECT IN B-CELL SURVIVAL (Romberg N, Morbach H, Lawrence MG, Kim S, Kang I, Holland SM, Milner
JD, Meffre E. J Allergy Clin Immunol 2013; 131: 1691-1693).
• HAPLOIDENTICAL HEMATOPOIETIC STEM CELL TRANSPLANTATION CAN LEAD TO VIRAL
CLEARANCE IN SEVERE COMBINED IMMUNODEFICIENCY (Slatter M, Nademi Z, Patel S, Barge D,
Valappil M, Brigham K, Hambleton S, Clark J, Flood T, Cant A, Abinun M, Gennery A. J Allergy Clin Immunol
2013; 131: 1691-1693).
• INHIBITION OF HUMAN B-CELL DEVELOPMENT INTO PLASMABLASTS BY HISTONE DEACETYLASE
INHIBITOR VALPROIC ACID (Kienzler AK, Rizzi M, Reith M, Nutt SL, Eibel H. J Allergy Clin Immunol 2013;
131: 1695-1699).
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
3. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• LESS SEVERE CLINICAL MANIFESTATIONS IN PATIENTS WITH HEREDITARY ANGIOEDEMA WITH
MISSENSE C1INH GENE MUTATIONS (Bors A, Csuka D, Varga L, Farkas H, Tordai A, Füst G, Szilagyi A. J
Allergy Clin Immunol 2013; 131: 1708-1711).
• MICROBIAL INFLUENCE ON TOLERANCE AND OPPORTUNITIES FOR INTERVENTION WITH
PREBIOTICS/PROBIOTICS AND BACTERIAL LYSATES (Pfefferle PI, Prescott SL, Kopp M. J Allergy Clin
Immunol 2013; 131: 1453-1463).
• NEWBORN SCREENING FOR SEVERE COMBINED IMMUNODEFICIENCY DOES NOT IDENTIFY BARE
LYMPHOCYTE SYNDROME (Kuo CY, Chase J, Garcia Lloret M, Stiehm ER, Moore T, Matas Aguilera MJ,
Lopez Siles J, Church JA. J Allergy Clin Immunol 2013; 131: 1693-1695).
• PRIMARY PREVENTION OF ALLERGY BY USING PROTEIN HYDROLYSATE: AN ACHIEVABLE
OBJECTIVE? (Arshad SH. J Allergy Clin Immunol 2013; 131: 1574-1575).
• REDUCED SEROLOGIC RESPONSE TO MUMPS, MEASLES, AND RUBELLA VACCINATION IN
PATIENTS TREATED WITH INTRAVENOUS IMMUNOGLOBULIN FOR KAWASAKI DISEASE (Tacke CE,
Smits GP, van der Klis FRM, Kuipers IM, Zaaijer HL, Kuijpers TW. J Allergy Clin Immunol 2013; 131: 1701-
1703).
• SERUM TRYPTASE DETERMINATION IN PATIENTS WITH ACUTE ALLERGIC REACTIONS (Vitte J,
Bongrand P. J Allergy Clin Immunol 2013; 131: 1714).
• TREATMENT OF HYPOGAMMAGLOBULINEMIA IN ADULTS: A SCORING SYSTEM TO GUIDE
DECISIONS ON IMMUNOGLOBULIN REPLACEMENT (Agarwal S, Cunningham-Rundles Ch. J Allergy Clin
Immunol 2013; 131: 1699-1701).
• ALLERGIC CONTACT DERMATITIS IN CHILDREN: WHICH FACTORS ARE RELEVANT? (REVIEW OF
THE LITERATURE) (de Waard-van der Spek FB, Andersen KE, Darsow U, Mortz CG, Orton D, Worm M,
Muraro A, Schmid-Grendelmeier P, Grimalt R, Spiewak R, Rudzeviciene O, Flohr C, Halken S, Fiocchi A,
Borrego LM, Oranje AP. Pediatr Allergy Immunol 2013: 24: 321–329).
• BARLEY’S LIPID TRANSFER PROTEIN: A NEW EMERGING ALLERGEN IN PEDIATRIC ANAPHYLAXIS
(Nemni A, Borges JP, Rougé P, Barre A, Just J. Pediatr Allergy Immunol 2013: 24: 410–411).
• COMPARISON BETWEEN TWO MAINTENANCE FEEDING REGIMENS AFTER SUCCESSFUL COW’S
MILK ORAL DESENSITIZATION (Pajno GB, Caminiti L, Salzano G, Crisafulli G, Aversa T, Messina MF,
Wasniewska M, Passalacqua G. Pediatr Allergy Immunol 2013: 24: 376–381).
• CONSIDERING 25 YEARS OF RESEARCH ON ALLERGY PREVENTION – HAVE WE LET OURSELVES
DOWN? (Whan U. Pediatr Allergy Immunol 2013: 24: 308–310).
• IS THERE AN ASSOCIATION BETWEEN MICROBIAL EXPOSURE AND FOOD ALLERGY? A
SYSTEMATIC REVIEW (Marrs T, Bruce KD, Logan K, Rivett DW, Perkin MR, Lack G, Flohr C. Pediatr Allergy
Immunol 2013: 24: 311–320).
• ROLE OF SPECIFIC IGE IN PREDICTING THE CLINICAL COURSE OF LENTIL ALLERGY IN CHILDREN
(Yavuz ST, Sahiner UM, Buyuktiryaki B, Tuncer A, Yilmaz EA, Cavkaytar O, Karabulut E, Sackesen C. Pediatr
Allergy Immunol 2013: 24: 382–388).
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
4. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• ADHERENCE TO IMMUNOTHERAPY (IT) IN TIMES OF FINANCIAL CRISIS (González-de-Olano
D, Álvarez-Twose I. Ann Allergy Asthma Immunol 2013; 110: 466–468):
• Reasons for nonadherence to allergen IT: (i) inconvenience, (ii) high costs, (iii) lack of efficacy.
• Authors show in Spain that adherence to IT (especially to sublingual IT) ↓ during financial crisis.
• Factors associated with ↑ adherence to IT: (i) prescription before economic recession
(OR=1.89); (ii) patient age <12 yrs (OR=3.06).
• ALLERGIC REACTIONS AFTER IMMUNIZATION (Kelso GM. Ann Allergy Asthma Immunol 2013;
110: 397–401):
• Vaccines: ↓ morbidity and mortality of many infectious diseases (eg. eradication of smallpox).
• Considerations regarding adverse reactions to vaccines: (i) confirm the adverse reaction; (ii) if
the clinical history suggests an IgE-mediated reaction, perform in vivo and in vitro tests to detect
specific IgE (sIgE) against the vaccine or its components; (iii) patients with negative vaccine
skin tests will usually tolerate the vaccine; (iv) patients with positive vaccine skin tests may
tolerate the vaccine, in these cases the vaccine should be gradually administered; (v) it is
prudent to observe the patient 30 min after the immunization; (vi) it is prudent to be prepared for
anaphylaxis; (vii) consider assessing patient’s immune status to the vaccine (prior doses,
including the suspected culprit dose, may have generated enough immunity, so further doses
could be withheld or delayed); (viii) if an IgE-mediated reaction to the vaccine is confirmed, try
to detect the specific culprit allergen because other vaccines could contain the same allergen
(eg. a patient with gelatin allergy may react to MMR, varicella or influenza vaccines); (ix) in most
cases, patients with suspected allergy to vaccines can receive subsequent vaccinations safely.
• How to confirm an IgE-mediated allergy to a vaccine? (i) Suggestive clinical history:
manifestations of mast cell degranulation within 4 hrs after immunization; (ii) IgE detection by
skin testing (use the same vaccine brand that caused the reaction): SPT with undiluted vaccine,
intradermal reaction with 1/100 diluted vaccine (nonirritating concentration).
• How to confirm an IgE-mediated allergy to a vaccine component? (i) Suggestive clinical history:
signs of mast cell degranulation within 4 hrs after using a vaccine component (eg. egg, gelatin,
yeast, latex); (ii) sIgE detection to the vaccine component: SPT, in vitro testing; (iii) OFC.
• Gelatin: (i) stabilizer (μg to mg quantities) of many vaccines; (ii) bovine or porcine origin
(extensively cross-reactive); (iii) most frequent culprit allergen in vaccines.
• How to diagnose gelatin allergy? (i) Clinical history: ask for reactions after gelatin ingestion, a
negative history does not exclude gelatin allergy; (ii) sIgE detection in vitro; (iii) SPT with an
office-made extract (not approved by the FDA): dissolve 1 teaspoon of sugared gelatin powder
(any flavor) in 5 mL of normal saline (unsugared gelatin tends to gel at room temperature).
• How to approach a patient with IgE-mediated gelatin allergy? Perform skin testing with gelatin-
containing vaccines → (i) negative results → vaccinate the patient, observe 30 min afterward;
(ii) positive results → consider vaccination in graded doses under observation.
• Egg protein (ovalbumin): present in influenza vaccine (very low amounts) and yellow fever
vaccine (higher amounts).
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
5. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• How to diagnose egg allergy? (i) Clinical history: ask for reactions after egg ingestion; (ii) sIgE
detection by skin and serum tests; (iii) oral food challenge.
• How to approach a patient with IgE-mediated egg allergy who needs influenza vaccine? (i)
Administer an entire dose without previous skin tests, even in patients with anaphylaxis to egg;
(ii) observe 30 min after vaccination; (iii) be prepared to manage anaphylaxis; (iv) injectable
trivalent vaccine is preferred over nasal live attenuated vaccine because its safety in egg-
allergic patients has been studied more extensively; (v) 2 new influenza vaccines (not grown in
eggs) were recently approved for patients ≥18 yrs of age (Flucelvax and Flublok).
• How to approach a patient with IgE-mediated egg allergy who needs yellow fever vaccine?
Perform skin tests with the vaccine → (i) negative results → vaccinate the patient, observe 30
min afterward; (ii) positive results → consider vaccination in graded doses under observation.
• Yeast protein (Saccharomyces cerevisiae; common baker’s or brewer’s yeast): present in
hepatitis B vaccines (up to 25 mg per dose) and quadrivalent human papillomavirus vaccine (<7
μg per dose); yeast allergy is rare.
• How to diagnose yeast allergy? (i) Clinical history: ask for reactions after yeast ingestion; (ii)
sIgE detection by skin and serum tests to Saccharomyces cerevisiae.
• How to approach a patient with confirmed IgE-mediated yeast allergy? Perform skin testing with
yeast-containing vaccines → (i) negative results → vaccinate the patient, observe 30 min
afterward; (ii) positive results → consider vaccination in graded doses under observation.
• Natural rubber latex: present in the packaging of many vaccines (vial stopper, syringe); very low
risk of vaccine contamination with latex → minimal risk of allergic reactions in patients with IgE-
mediated latex allergy.
• How to approach a patient with IgE-mediated latex allergy? (i) Use a vaccine without latex
stopper; (ii) if not possible, remove the stopper and take the vaccine directly from the vial; (iii) if
latex packaging cannot be avoided (eg. a prefilled syringe), vaccinate and observe the patient
30 min afterward.
• http://www.cdc.gov/vaccines/pubs/pinkbook/downloads/appendices/B/latex-table.pdf
(information on latex and vaccine packaging).
• DISCORDANCE BETWEEN AEROALLERGEN SPECIFIC SERUM IGE AND SKIN TESTING IN
CHILDREN YOUNGER THAN 4 YEARS (de Vos G, Nazari R, Ferastraoaru D, Parikh P, Geliebter
R, Pichardo Y, Wiznia A, Rosenstreich D. Ann Allergy Asthma Immunol 2013; 110: 438–443):
• Risk factors for asthma in young children (<4 yrs old) with recurrent wheeze: (i) IgE-
sensitization to aeroallergens; (ii) parental history of allergies.
• Diagnosis of IgE-sensitization to aeroallergens: (i) skin prick tests (SPT); (ii) specific IgE (sIgE)
determination in vitro; (iii) challenge tests.
• A recent study showed ~80% concordance between SPT and sIgE testing results in adults with
rhinitis (Allergy Asthma Proc. 2009; 30: 386-396).
• Authors performed SPT (ComforTen device) and sIgE testing (Immulite 2000 3gAllergyT
system) to 7 aeroallergens (dog, grass, cockroach, cat, dust mite, ragweed, mouse) in 40
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
6. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
children (<4 yrs old) with recurrent wheezing and family history of asthma and/or eczema → (i)
in 80% of children ≥1 sensitizations would have been missed if only SPT had been done; (ii) in
38% of children ≥1 sensitizations would have been missed if only sIgE testing had been done;
(iii) children with total IgE ≥300 kU/L were more likely to have only positive sIgE test results.
• Author’s commentaries: (i) there was a significant discordance between SPT and sIgE testing
results (possible reasons: antigenic differences between testing materials, variability between
testing instruments, immaturity of immune system in young children [eg. mast cells may not
have trafficked to the epidermis in sufficient numbers to elicit a typical wheal-and-flare
response; mast cells may be less reactive]); (ii) both SPT and sIgE testing should be performed
to diagnose IgE-sensitization in young children at high risk of asthma.
• EFFICACY OF OMALIZUMAB IN ASTHMATIC PATIENTS WITH IGE LEVELS ABOVE 700 IU/ML:
A RETROSPECTIVE STUDY (Maselli DJ, Singh H, Diaz J, Peters JI. Ann Allergy Asthma Immunol
2013; 110: 457–461):
• Omalizumab: (i) anti-IgE mAb → binds to free IgE → ↓ IgE binding to its receptors → ↓ IgE-
mediated inflammation; (ii) approved for [uncontrolled asthma + serum IgE levels between 30
and 700 IU/mL + sensitization to perennial allergens]; (iii) dose is calculated in a chart, based
on pretreatment IgE levels (between 30 and 700 IU/mL) and body weight; alternative formula
when the chart is not suitable: ≥0.016 mg/kg per IgE unit every 4-wk period; suggested
maximum dose: 750 mg every 4 wks.
• Authors retrospectively studied 26 asthmatic patients (12-67 yrs old) with IgE levels >700 IU/mL
who received omalizumab for ≥6 months → omalizumab efficacy was similar compared with a
matched group of patients with IgE between 30 and 700 IU/mL: (i) ↑ asthma control; (ii) ↓ ED
visits; (iii) ↓ systemic corticosteroid use; (iv) no change in mean FEV1; (v) good safety profile.
• OXYTOCIN: A LIKELY UNDERESTIMATED RISK FOR ANAPHYLACTIC REACTIONS IN
DELIVERING WOMEN SENSITIZED TO LATEX (Liccardi G, Bilò MB, Mauro C, Salzillo A, Piccolo
A, D’Amato M, D’Amato G. Ann Allergy Asthma Immunol 2013; 110: 465–466):
• Oxytocin: drug that ↑ uterine contractility; adverse effects: cardiovascular side effects,
bronchoconstriction (asthma worsening), rare cause of anaphylaxis during delivery.
• Latex allergy: risk factor for anaphylaxis to oxytocin (homology between oxytocin epitopes and
latex allergens Hev b 7.01 and Hev b 7.02 [patatin]).
• Authors report 2 latex-sensitized women (37 and 43 yrs old) with severe anaphylaxis after
oxytocin infusion during delivery → diagnosis: positive SPT with Syntocinon 5UI /mL; positive
SPT and sIgE in vitro testing with latex.
• Author’s commentaries: (i) in this case, not testing to oxytocin could have led to an incomplete
diagnosis of latex allergy → after an intraoperative anaphylaxis it is recommended to test all
drugs or substances that were used; (ii) latex-allergic women should be carefully managed
during delivery (latex-free materials, oxytocin alternative agents, appropriate asthma control).
• PEDIATRIC SUBLINGUAL IMMUNOTHERAPY (SLIT) EFFICACY: EVIDENCE ANALYSIS, 2009-
2012 (Larenas-Linnemann D, Blaiss M, Van Bever HP, Compalati E, Baena-Cagnani CE. Ann
Allergy Asthma Immunol 2013; 110: 402–415):
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
7. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• Allergen immunotherapy: only therapy with the potential to modify the natural history of allergic
diseases; objectives: restore tolerance, prevent new sensitizations, stop ‘atopic march’; SLIT
route of administration: safer, avoids injection pain, more suitable for children.
• Authors analyzed (by the GRADE tool) 29 recent (2009-2012) clinical trials (2469 patients)
about SLIT efficacy in children (≤18 yrs old) with respiratory and/or food allergies → (i) grass
pollen SLIT is effective in seasonal allergic rhinitis and may be effective in asthma; (ii) HDM
SLIT seems to be effective, especially in asthma; (iii) there is not enough evidence to
recommend Alternaria SLIT; (iv) food oral IT is more promising than food SLIT; (v) SLIT has
good safety profile in respiratory allergies; (vi) be careful with side effects during food IT; (vii)
further high-evidence research is needed.
• AN ALTERNATIVE EXPLANATION FOR THE INVERSE RELATIONSHIP BETWEEN ATOPY AND
MYOCARDIAL INFARCTION (Varner A. J Allergy Clin Immunol 2013; 131: 1715):
• A recent report showed an inverse relationship between allergen-specific IgE and myocardial
infarction. Proposed mechanisms: (i) ↑ specific IgE → ↑ TH2 inflammation → ↓ TH1
inflammation → ↓ CV disease; (ii) atopic patients may have platelet dysfunction and ↑ bleeding
times → ↓ CV disease.
• By the other side, ↑ total IgE has been related to ↑ myocardial infarction. Proposed mechanism:
↑ total IgE → IgE occupies FcεRI and FcεRII on platelets → platelets are activated by
allergens → ↑ CV disease.
• Recently, the FDA reported that omalizumab was related to ↑ risk of thrombotic and CV events.
• APPROVED AND UNAPPROVED USE OF IMMUNOGLOBULINS IN ICELAND (Ólafsdóttir BE,
Porsteinsson DP, Einarsdóttir R, Ludvíksson BR, Gröndal G, Erlendsson K, Haraldsson A. J Allergy
Clin Immunol 2013; 131: 1703-1705):
• Immunoglobulin: (i) replacement therapy for immunodeficiencies, (ii) immunomodulatory
therapy for autoimmune and inflammatory diseases. Approved and off-label indications include
>150 different conditions.
• Authors describe the use of immunoglobulin therapy in Iceland during years 2001 to 2009 → (i)
389 patients received IVIG, 13 received SCIG; (ii) approved indications = 264, unapproved =
110, unclear = 28; (iii) most common indications: PIDs, multiple myeloma, leukemias,
lymphomas, demyelinating diseases, multiple sclerosis, idiopathic thrombocytopenia, Kawasaki
disease, systemic lupus erythematosus, asthma.
• EFFECT OF BARRIER MICROBES ON ORGAN-BASED INFLAMMATION (Garn H, Neves JF,
Blumberg RS, Renz H. J Allergy Clin Immunol 2013; 131: 1465-1478):
• Intestinal microbiota (>100 trillion microorganisms; Firmicutes and Bacteroidetes phyla
predominate) → development and regulation of the immune system, mostly in the neonatal
period (eg. TLR2 activation by microbes in a ‘tolerogenic’ environment promotes Treg
development; ‘tolerogenic’ microbiota promotes IgA production) → local homeostasis
(tolerance), distal homeostasis? (eg. in the lung).
• Factors that may disturb microbiota: (i) Cesarean delivery; (ii) no breastfeeding; (iii) inadequate
diet; (iv) early use of antibiotics; (v) low exposure to farms; (vi) certain infections.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
8. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• Modern/industrialized lifestyle → dysbiosis (qualitative and quantitative disturbance of the
microbiota) → dysfunctional immune responses (loss of tolerance) → ↑ risk of allergic (eg.
asthma), autoimmune and inflammatory disorders (eg. IBD).
• Inflammatory bowel disease (IBD): altered gut microbiota (↓ Bacteroidetes and Firmicutes, ↑
Actinobacteria and Proteobacteria) → exaggerated immune response against commensal
microbiota and potentially against host tissues → chronic inflammation.
• Allergic diseases: altered gut and/or respiratory microbiota (less diversity, altered predominance
of species) → loss of tolerance to foreign allergens → TH2 inflammation.
• It’s not fully defined: (i) which microbes protect from inflammatory diseases (lactobacilli and
bifidobacterium seem to have beneficial effects); (ii) the direction of causality between
inflammatory diseases and altered microbiota.
• How can we correct dysbiosis? (i) Natural delivery; (ii) breastfeeding; (iii) adequate nutrition; (iv)
correct use of antibiotics; (v) probiotics and prebiotics; (vi) short-chain fatty acids.
• ENDOTYPES AND PHENOTYPES OF CHRONIC RHINOSINUSITIS: A PRACTALL DOCUMENT
OF THE EUROPEAN ACADEMY OF ALLERGY AND CLINICAL IMMUNOLOGY AND THE
AMERICAN ACADEMY OF ALLERGY, ASTHMA & IMMUNOLOGY (Akdis CA, Bachert C, Cingi C,
Dykewicz MS, Hellings PW, Naclerio RM, Schleimer RP, Ledford D. J Allergy Clin Immunol 2013;
131: 1479-1490):
• Sinusitis rarely occurs in the absence of rhinitis.
• Rhinosinusitis (RS): inflammation of nasal and paranasal mucosa; diagnosis: (i) nasal blockage
or nasal discharge (anterior or posterior) + facial pain/pressure or hyposmia, (ii) objective
clinical, endoscopic or radiologic evidence of sinonasal inflammation (polyps, mucopurulent
discharge, edema). Acute RS: <12 wks. Chronic RS (CRS): ≥12 wks.
• Pathogenesis of CRS: (i) Anatomic/structural problems: nasal septal deviation, nasal valve
dysfunction, concha bullosa (enlarged nasal turbinate caused by internal ethmoid air cell),
adenoid hyperplasia (mainly in children), nasal choanal narrowing, nasal or sinus mucoceles,
scarring from prior nasal or sinus surgery, septal perforations, nasal foreign body, malignancies.
• (ii) Altered immunity: altered epithelial barrier (↓ epithelial tight junctions [occludins, claudins,
etc], ↑ epitelial shedding, ↓ repairing proteins [psoriasin, calgranulin A and B, SPINK5]); ↓
ciliary clearance (cystic fibrosis, primary ciliary dyskinesia); ↓ antimicrobial peptides (defensins,
psoriasin, PLUNC family, lysozyme, lactoferrin); ↓ TLR responses (especially TLR2 and TLR9);
↓ STAT3 function; ↑ immune activation (T-cell responses [TH2, TH17, TH22], IgE, IgA, effector
cells [mast cells, eosinophils], autoantibodies, IL-32); immunodeficiencies (eg.
agammaglobulinemia); inflammatory diseases (eg. Wegener’s granulomatosis, sarcoidosis,
aspirin-exacerbated respiratory disease); ↑ remodeling (TGF-β, MMPs, TIMPs).
• (iii) Infections (bacteria, fungi, virus, biofilms): role is not fully defined → antibiotics may help
(macrolides and doxycycline have been suggested due to antibacterial and antiinflammatory
properties); biofilm-destabilizing agents may help; some patients respond to antifungals.
• (iv) Pollutants and drugs: cigarette smoke, pollutants, cocaine, topical vasoconstrictors.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
9. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• Treatment of CRS: (i) nasal irrigation/douching; (ii) intranasal and oral corticosteroids; (iii)
antibiotics; (iv) surgery; (v) other therapies: antihistamines, LTRA, 5-lipoxygenase inhibitors,
allergen immunotherapy, biological agents (monoclonal antibodies, soluble receptors,
cytokines), aspirin desensitization, topical and oral antifungals, decongestants, mucolytics (eg.
n-acetylcysteine), phototherapy, MTX, protein pump inhibitors, capsaicin, furosemide, vit D,
Manuka honey, bromelain, quercetin, undecylenic acid, urtica dioica, massage of the sinus
ostea with swabs of botanical essential oils, air purifiers, diets.
• Phenotype: observable characteristics of a disease. Endotype: pathophysiologic mechanisms of
a disease.
• Modern medicine: determination of specific phenotypes and endotypes of a disease →
personalized therapy → improved outcomes.
• Advantages of endotyping: (i) facilitates development of biomarkers for diagnosis and
prognosis; (ii) facilitates association to genetic and environmental factors; (iii) improves
personalized therapy.
• CRS phenotypes: (i) CRS with nasal polyps (CRSwNP): 4% of the population, TH2
environment, responds better to intranasal corticosteroids; (ii) CRS without nasal polyps
(CRSsNP): ↑ remodeling (TGF-β, MMP, TIMP, collagen).
• Possible CRS endotypes: (i) fungal-induced endotype (eg. allergic fungal RS); (ii) S. aureus-
induced endotype (superantigens favor TH2 milieu; IL-4 and IL-13 ↓ immunity to S aureus); (iii)
mucosal barrier defect endotype; (iv) innate immune defect endotype; (v) TH2/IgE/eosinophilic
endotype; (vi) TH17/neutrophilic endotype; (vii) autoimmune endotype; (viii) drug-induced
endotype (eg. AERD); (ix) remodeling endotype.
• EXPANDING THE PARADIGM OF EOSINOPHILIC ESOPHAGITIS: MAST CELLS AND IL-9
(Wang YH, Hogan SP, Fulkerson PC, Abonia JP, Rothenberg ME. J Allergy Clin Immunol 2013; 131:
1583-1585):
• Eosinophilic esophagitis (EoE): eosinophil infiltration into esophageal mucosa → inflammation
(vomiting, dysphagia, abdominal pain, failure to thrive, low response to acid-suppressive
therapy); probable cause: exaggerated immune response to food allergens or aeroallergens;
pathogenic factors: (i) ↑ CCL26 (eotaxin-3) → action through CCR3 → ↑ eosinophil attraction;
(ii) ↑ IL-5 → ↑ eosinophil production, attraction, activation and survival.
• Otani et al (J Allergy Clin Immunol 2013; 131: 1576-82): beneficial effect of anti–IL-5 treatment
in EoE was related to ↓ in mast cell/eosinophil couplets (esophageal eosinophils and adjacent
tryptase-positive mast cells).
• New contribution to EoE pathogenesis: eosinophils produce IL-9 → ↑ mast cell infiltration and
activation → interplay between eosinophils and mast cells can be associated with EoE severity.
• Which cells produce IL-9? (i) TH2 cells; (ii) TH9 cells (transcription factors: GATA3, PU.1,
IRF4); (iii) type 2 cytokine–producing innate lymphoid cells (ILC2, natural helper cells or
nuocytes); (iv) eosinophils.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
10. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• Stimulus for IL-9 production: TGF-β (produced by mast cells, eosinophils, Tregs) + IL-4
(produced by TH2 cells, ILC2, basophils) → TH9 cell differentiation; IL-9 production by
eosinophils and ILC2.
• GAIN-OF-FUNCTION (GOF) STAT1 MUTATIONS ARE ASSOCIATED WITH PD-L1
OVEREXPRESSION AND A DEFECT IN B-CELL SURVIVAL (Romberg N, Morbach H, Lawrence
MG, Kim S, Kang I, Holland SM, Milner JD, Meffre E. J Allergy Clin Immunol 2013; 131: 1691-1693):
• GOF mutations in STAT1 → ↑ response to interferons and IL-27 → TH17-cell deficiency,
chronic mucocutaneous candidiasis (CMC), susceptibility to certain bacterial infections,
autoimmunity. New described phenotypes include IPEX-like syndrome and disseminated
coccidioidomycosis and histoplasmosis.
• Authors report 4 related subjects with a novel GOF mutation in the coiled-coil domain of STAT1.
• Patient 1 (60 yrs old): CMC; lung infections by P aeruginosa, S pneumoniae, Serratia sp, M
avium and RSV; HPV-positive squamous cell carcinoma of palate; basal cell carcinoma;
shingles; fibromuscular dysplasia with carotid and celiac/splenic artery dissection; IgG2
subclass deficiency that progressed into frank hypogammaglobulinemia; B-cell lymphopenia.
• Patient 2 (30 yrs old, daughter of patient 1): CMC; pneumonia; chronic bronchitis; otitis media;
sinusitis; B-cell lymphopenia; IgG2 subclass deficiency.
• Patients 3 (6 wks old) and 4 (24 months old): children of patient 2; both carry the GOF STAT1
mutation but have no manifestations of immunodeficiency.
• Remarkable laboratory findings: (i) STAT1 hyperphosphorylation in T cells after stimulus with
IL-21; (ii) ↓ IL-17A–secreting CD4+ cells; (iii) overexpression of PD-L1 on naive CD4+ T cells;
(iv) ↑ B-cell apoptosis (Annexin V and 7-AAD staining; caspase activity); (v) humoral deficiency.
• Hypothesis: STAT1 GOF mutations → ↑ response to IL-27 → overexpression of PD-L1 on
naive T cells after stimulation with IL-27 → ↑ PD-1/PD-L1 interaction → ↓ TH17 commitment,
↓ TFH help to B cells?
• HAPLOIDENTICAL HEMATOPOIETIC STEM CELL TRANSPLANTATION CAN LEAD TO VIRAL
CLEARANCE IN SEVERE COMBINED IMMUNODEFICIENCY (SCID) (Slatter M, Nademi Z, Patel
S, Barge D, Valappil M, Brigham K, Hambleton S, Clark J, Flood T, Cant A, Abinun M, Gennery A. J
Allergy Clin Immunol 2013; 131: 1691-1693):
• SCID requires urgent therapy with HSCT → (i) ideally, HSCT should be performed with an
HLA-identical donor; (ii) if an identical donor is not available, T-lymphocyte–depleted (TCD)
HSCT from an HLA-mismatched donor can be performed (75-87% survival); (iii) T-cell depletion
↓ risk of GVHD; (iv) B-cell depletion ↓ risk of EBV-related lymphoproliferation.
• Authors report 5 virus-infected SCID patients who received parental HLA-haploidentical TCD
HSCT (2 received CD34+
–selected marrow; 3 received CD3/CD19-depleted peripheral blood
stem cells) → 4 patients achieved immunoreconstitution and viral clearance; 1 patient died.
• TCD HSCT can quickly restore immunity to achieve viral clearance in SCID patients.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
11. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• INHIBITION OF HUMAN B-CELL DEVELOPMENT INTO PLASMABLASTS BY HISTONE
DEACETYLASE INHIBITOR VALPROIC ACID (Kienzler AK, Rizzi M, Reith M, Nutt SL, Eibel H. J
Allergy Clin Immunol 2013; 131: 1695-1699):
• Naive B cells encounter microbial antigen for the 1st
time and differentiate into: (i) short-lived
plasmablasts, which produce early protective immunoglobulins; (ii) long-lived plasma cells,
which maintain long-term immunoglobulin production; (iii) memory B cells, which quickly
activate after repetitive infection.
• B cell differentiation is strictly linked to proliferation.
• Histone deacetylase (HDAC) inhibitors: novel anticancer drugs (regulate proliferation and
apoptosis of tumor cells); immunomodulatory properties at low doses (↓ T-cell proliferation, ↑
TH2 cytokine production, ↑ Treg function).
• Valproic acid (VPA): antiepileptic drug; inhibits class I (including HDAC1 and HDAC2) and class
IIa HDACs → ↓ migratory capacity of dendritic cells, ↓ T-cell activation → ↓ experimental
autoimmune diseases.
• Authors studied the in vitro effect of VPA (at low therapeutic concentration: 0.25 mmol/L = 42
mg/L) in human B-cell activation and differentiation → (i) without VPA, during 9 days of
cultivation with CD40L + IL-21, naive B cells gradually differentiated into plasmablasts (CD27high
CD38high
); (ii) VPA ↓ naive B-cell proliferation and differentiation into plasmablasts in a dose-
dependent manner; (iii) VPA did not ↓ memory B-cell reactivation and differentiation into
plasmablasts; (iv) VPA did not ↓ naive B-cell survival; (v) VPA ↓ IgM, IgG and IgA production
from stimulated naive B cells, but not from stimulated memory B cells; (vi) VPA did not change
CD40, IL-21R, CD86, AICDA, JAK3, STAT3 or STAT5 expression or phosphorylation in
activated naive B cells; (viii) VPA ↓ T-cell proliferation induced by CD3 and CD28 stimulation.
• Author’s commentaries: (i) previous reports showed antibody defects in children, but not adults,
taking VPA → hypothesis: adults have more memory B cells, children have more naive B cells
(VPA affects proliferation and differentiation of naive B cells, but not memory B cells); (ii) B-cell
responses against new encountered microbes and vaccines may be ↓ in children taking VPA;
(iii) HDAC inhibitors may help in autoimmune diseases (eg. in SLE, 60% of the autoantibodies
against nuclear antigens are produced by autoreactive short-lived plasma cells).
• LESS SEVERE CLINICAL MANIFESTATIONS IN PATIENTS WITH HEREDITARY ANGIOEDEMA
(HAE) WITH MISSENSE C1INH GENE MUTATIONS (Bors A, Csuka D, Varga L, Farkas H, Tordai
A, Füst G, Szilagyi A. J Allergy Clin Immunol 2013; 131: 1708-1711):
• HAE → type I: ↓ level of C1 inhibitor (C1INH); type II: ↓ function of C1INH → recurrent attacks
of bradykinin-mediated angioedema, potentially life-threatening.
• Authors studied 106 subjects with type I HAE confirmed by genetic sequencing (mutations of
the SERPING1 gene) → a) patients with missense mutations had: (i) onset of symptoms at
older age, (ii) fewer total angioedema attacks, (iii) fewer severe angioedema attacks, (iv) less
use of C1INH concentrate; b) a polymorphism in factor XII gene was associated with higher
factor XII levels and earlier onset of symptoms in type I HAE patients.
• Author’s commentary: the type of mutation in SERPING1 gene may influence the clinical course
of patients with type I HAE → more personalized therapy?
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
12. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• MICROBIAL INFLUENCE ON TOLERANCE AND OPPORTUNITIES FOR INTERVENTION WITH
PREBIOTICS/PROBIOTICS AND BACTERIAL LYSATES (Pfefferle PI, Prescott SL, Kopp M. J
Allergy Clin Immunol 2013; 131: 1453-1463):
• Exaggerated immune responses: (i) to self antigens: autoimmune diseases; (ii) to beneficial or
innocuous foreign antigens: allergic diseases.
• Microbiota: all microorganisms colonizing epithelial surfaces.
• ‘Tolerogenic’ microbiota: microorganisms that: a) regulate immune system development and
homeostasis; b) promote tolerance in the early life to: (i) self antigens; (ii) beneficial or
innocuous foreign antigens.
• Factors that influence microbiota in the offspring: (i) during pregnancy: maternal diet, maternal
environment, maternal microbiota; (ii) during partum: type of delivery, use of antibiotics; (iii)
during postnatal period and infancy: breastfeeding, diet, use of antibiotics, microbial
environment (eg. exposure to farms), pets.
• Which microbes are ‘tolerogenic’? Not well defined; likely Lactobacillus and Bifidobacterium sp.
• Factors that may disturb ‘tolerogenic’ microbiota: (i) altered microbiota in the mother; (ii)
Cesarean delivery; (iii) insufficient breastfeeding; (iv) inadequate diet; (v) antibiotic use.
• How do maternal environmental exposures increase allergy risk in the offspring? Hypothesis: (i)
epigenetic modifications (eg. ↑ methylation of FOXP3 in germinal cells); (ii) altered cytokine
balance (eg. TH2 predominance, Treg deficiency); (iii) altered immune responses in the
placenta (eg. ↑ methylation of FOXP3 in the placenta).
• Products that may promote or restore ‘tolerogenic microbiota’ and tolerance: (i) probiotics:
‘tolerogenic’ bacteria (eg. Lactobacillus rhamnosus, Bifidobacterium sp); (ii) prebiotics: nutrients
that promote ‘tolerogenic’ bacteria (eg. FOS, GOS).
• Evidence about probiotics: (i) around half of the studies using probiotics (during pregnancy
and/or early postnatal period) show ↓ eczema incidence (25-50% reduction); other studies
show no benefit, even with similar probiotic strains and protocols; (ii) probiotics have not shown
consistent effects on allergic sensitization or respiratory allergies; (iii) several meta-analyses
agree that probiotics ↓ eczema risk but not other allergic outcomes; (iv) effects of maternal
probiotic use on cord blood immune responses are conflicting; (v) good safety profile.
• Limitations to analyze study results about probiotics: (i) methodological differences between
studies; (ii) different probiotic strains were used, including mixes; (iii) different ways of
administration; (iv) different timing (prenatal, postnatal or both); (v) different doses; (vi) different
clinical outcomes; (vii) different population characteristics; (viii) limited follow-up of the patients;
(ix) high dropout rate in several studies.
• Benefits of prebiotic use during pregnancy: (i) possible ↓ in some allergic manifestations; (ii)
probable benefit in chronic metabolic diseases (obesity, diabetes); (iii) ↓ in constipation.
• Current evidence shows that prebiotics and probiotics might contribute to prevention of atopic
eczema but not asthma, allergic rhinitis or allergic sensitization. A combined antenatal and
postnatal use of probiotics has been the most promising approach in high-risk children.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
13. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• Uncertainties for research: (i) which probiotic strain or prebiotic product should be used? (so far,
L rhamnosus had the best results) (ii) when? (iii) how much? (iv) for how much time?
• Bacterial lysates: lyophilized extracts from single-strain or mixed bacterial cultures
(aeropathogenic or endotoxin-containing gut bacteria) through chemical or mechanical lysis.
• Evidence about bacterial lysates from aeropathogenic bacteria: (i) may ↑ secretory IgA levels
against bacteria; (ii) may skew perinatal TH2 milieu to TH1/Treg milieu; (iii) may ↓ acute
respiratory infections; (iv) may ↓ asthma initiation and exacerbations; (iv) good safety profile.
• Evidence about bacterial lysates from endotoxin-containing gut bacteria: (i) may ↓ eczema risk
in children with parental history of allergy.
• Manipulation of the microbiome could prevent: (i) allergic and autoimmune phenomena; (ii)
metabolic diseases; (iii) mood and behavior diseases.
• NEWBORN SCREENING FOR SEVERE COMBINED IMMUNODEFICIENCY DOES NOT IDENTIFY
BARE LYMPHOCYTE SYNDROME (Kuo CY, Chase J, Garcia Lloret M, Stiehm ER, Moore T, Matas
Aguilera MJ, Lopez Siles J, Church JA. J Allergy Clin Immunol 2013; 131: 1693-1695):
• Severe combined immunodeficiencies (SCID): genetic defects characterized by marked ↓ of T-
cell development and function; susceptibility to opportunistic infections; require urgent therapy
with HSCT or gene therapy; newborns can be screened for SCID by measuring TRECs (T-cell
receptor excision circles).
• Combined immunodeficiencies (CID), also called leaky-SCID or T+
SCID: profound T-cell
dysfunction with significant numbers of circulating T cells.
• TREC analysis: excellent sensitivity to detect SCID; CID may not be detected.
• MHC class II deficiency (bare lymphocyte syndrome): autosomal recessive CID; 4 disease-
causing genes (RFXANK, RFXAP, RFX5 and CIITA); CD4 T-cell lymphopenia; fatal course
without HSCT; TREC levels in this condition have not been reported.
• Authors report 2 infants with MHC class II deficiency who had normal TREC levels at birth (142
and 97 copies; normal values ≥40 copies).
• Patient’s characteristics: (i) early presentation (3 and 6 months of age) with failure to thrive,
intractable diarrhea and severe respiratory infections; one patient had severe neurologic
impairment (likely associated with a homozygous deletion in CTNND2 on chromosome 5p15.2);
(ii) profound hypogammaglobulinemia; (iii) ↓ CD4 T-cells; (iv) ↓ lymphocyte proliferation; (v) ↓
HLA-DR expression on peripheral blood lymphocytes; (vi) genetic mutations associated with
MHC class II deficiency; (vii) one patient died after HSCT, the other patient was not candidate
for HSCT because of severe lung and neurologic disease.
• Author’s commentary: patients with MHC class II deficiency and other CID may not be detected
by newborn screening using TRECs.
• PRIMARY PREVENTION OF ALLERGY BY USING PROTEIN HYDROLYSATE: AN ACHIEVABLE
OBJECTIVE? (Arshad SH. J Allergy Clin Immunol 2013; 131: 1574-1575):
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
14. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• Allergic diseases have dramatically increased. Proposals to stop this trend: (i) avoid allergen;
(ii) give allergen at an early age; (iii) restore tolerance to allergens; (iv) create a ‘tolerogenic
environment’ (probiotics, prebiotics, vit A, vit D, breastfeeding, omega-3 fatty acids).
• Some high-risk infants (parent or sibling with a history of allergy) cannot receive enough
breastfeeding at their first months of life → how to feed them to prevent allergy? Proposals: (i)
cow’s milk; (ii) partially hydrolyzed formulas (HFs): most peptides with molecular weights <10
kDa; (iii) extensively HFs: 95% of peptides with molecular weights <1.5 kDa; (iv) soy formulas.
• German Infant Nutritional Intervention (GINI): largest RCT investigating the primary preventive
effect of HFs on allergic manifestations → 2252 high-risk infants were assigned to 1 of 4
formulas in the first 4 months of life if breastfeeding was not feasible: (i) cow’s milk (CM), (ii)
partially hydrolyzed whey formula (pHF-W), (iii) extensively hydrolyzed whey formula (eHF-W),
(iv) extensively hydrolyzed casein formula (eHF-C) →
• Results: (i) at 1 yr of age: eHF-C group had ↓ atopic dermatitis (AD), GI food allergy and allergic
urticaria compared with CM group; pHF-W group had ↓ AD compared with CM group; (ii) at 3
yrs of age: eHF-C and pHF-W groups had ↓ AD compared with CM group; no effect on asthma;
(iii) at 6 yrs of age: similar preventive effect on AD; (iv) at 10 yrs of age: no ongoing protective
effect on AD between the ages of 7 and 10 yrs; eHF-C and pHF-W groups had ↓ cumulative
incidence of AD, primarily driven by the protective effect observed in the first 6 yrs of life.
• Conclusions: (i) eHF-C advantages: most effective (7 infants need to be treated to prevent 1
case of AD); (ii) pHF-W advantages: less expensive and more palatable; (iii) no evidence that
HFs are better than maternal milk; (iv) no evidence on prevention of asthma, allergic rhinitis or
allergen sensitization; (v) protective mechanisms of HFs are not clear.
• Limitations of the trial: (i) high dropout rate (64.4%); (ii) data at 1 and 3 yrs was based on
investigator’s evaluations; data at 6 and 10 yrs was based on parent’s reporting.
• REDUCED SEROLOGIC RESPONSE TO MUMPS, MEASLES, AND RUBELLA (MMR)
VACCINATION IN PATIENTS TREATED WITH INTRAVENOUS IMMUNOGLOBULIN (IVIG) FOR
KAWASAKI DISEASE (KD) (Tacke CE, Smits GP, van der Klis FRM, Kuipers IM, Zaaijer HL,
Kuijpers TW. J Allergy Clin Immunol 2013; 131: 1701-1703):
• KD: high risk of coronary aneurisms if untreated; therapy includes high-dose IVIG.
• IVIG is taken from thousands of donors to amplify the antibody repertoire → passively acquired
antibodies may interfere with immune response to vaccines.
• Authors evaluated the antibody response to MMR vaccination in 198 patients (1-9 yrs old)
treated with IVIG for KD (protective antibody levels: ≥0.2 IU/mL for measles, ≥10 IU/mL for
rubella, ≥45 RU/mL for mumps) → (i) compared to healthy controls, patients vaccinated within
6 months after IVIG had ↓ antibody levels against measles, mumps and rubella; (ii) patients
vaccinated between 6 and 9 months after IVIG had ↓ levels only against measles; (iii) patients
vaccinated >9 months after IVIG had similar antibody responses than healthy controls.
• Author’s commentaries: (i) MMR vaccination should be postponed at least 9 months after IVIG
use; (ii) the 11-month interval recommendation by the Advisory Committee on Immunization
Practices and the American Academy of Pediatrics may be longer than strictly necessary.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
15. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• SERUM TRYPTASE DETERMINATION IN PATIENTS WITH ACUTE ALLERGIC REACTIONS
(Vitte J, Bongrand P. J Allergy Clin Immunol 2013; 131: 1714):
• How to diagnose anaphylaxis? (i) clinical criteria; (ii) laboratory criteria: serum mediators
(tryptase, histamine, platelet-activating factor [PAF]) produced by allergy effector cells.
• Authors discuss the limitations of a recent study (Vadas et al. J Allergy Clin Immunol 2013; 131:
144-9), which concluded that PAF levels correlated better with anaphylaxis severity than
tryptase or histamine levels.
• Author’s commentaries: (i) each patient’s peak tryptase level must be compared with his/her
own basal tryptase level (anaphylaxis may ↑ serum tryptase levels as little as 135% or 2 mg/L);
(ii) tryptase levels should be measured between 30 to 120 min after anaphylaxis onset (serum
tryptase half-life is about 2 hrs); (iii) food-induced anaphylaxis may not ↑ tryptase levels.
• TREATMENT OF HYPOGAMMAGLOBULINEMIA IN ADULTS: A SCORING SYSTEM TO GUIDE
DECISIONS ON IMMUNOGLOBULIN REPLACEMENT (Agarwal S, Cunningham-Rundles Ch. J
Allergy Clin Immunol 2013; 131: 1699-1701):
• B-cell defects: (i) severe defects (eg. agammaglobulinemia) → replacement with IV or SC
immunoglobulin; (ii) modest defects → which patients should receive immunoglobulin?
• Limitations of immunoglobulin substitution: (i) expensive products; (ii) lifelong therapy.
• Authors describe a retrospectively-designed scoring system based on laboratory (IgG, IgA, IgM,
specific antibody responses) and clinical data (infections, lymphoproliferation, autoimmune and
inflammatory diseases, failure to thrive, bronchiectasis, lung function tests), which may help to
decide which adult patients with modest humoral defects should receive immunoglobulin.
• Author’s commentaries: (i) this score requires validation, standardization and proof of concept in
larger populations of patients with hypogammaglobulinemia; (ii) this score might need
modifications before applying to a pediatric population.
• ALLERGIC CONTACT DERMATITIS IN CHILDREN: WHICH FACTORS ARE RELEVANT?
(REVIEW OF THE LITERATURE) (de Waard-van der Spek FB, Andersen KE, Darsow U, Mortz CG,
Orton D, Worm M, Muraro A, Schmid-Grendelmeier P, Grimalt R, Spiewak R, Rudzeviciene O, Flohr
C, Halken S, Fiocchi A, Borrego LM, Oranje AP. Pediatr Allergy Immunol 2013: 24: 321–329):
• Risk factors for allergic contact dermatitis (ACD): (i) atopic dermatitis; (ii) skin barrier defects
(eg. filaggrin defects?); (iii) repetitive contact with potential allergens.
• When to suspect ACD? Chronic recurrent or therapy-resistant eczema, even in young children.
• Contact allergens in children: (i) metals: nickel (most common contact allergen), cobalt,
chromate; (ii) preservatives, solvents, emulsifiers; (iii) rubber chemicals; (iv) topical drugs
(chlorhexidine, neomycin, steroids, emollients, natural remedies); (v) fragrances.
• Where are contact allergens frequently encountered? (i) shoes: potassium dichromate, p-
phenylenediamine (PPD), p-tert-butylphenol-formaldehyde (PTBF) resin; (ii) perfumes; (iii)
jewelries: nickel; (iv) cosmetics; (v) temporary or permanent tattoos: PPD; (vi) shin guards:
rubber chemicals, thiourea derivates; (vii) hair dyes: PPD; (viii) clothing: dyes (often involve
thighs), formaldehyde (‘wrinkle resistant’), rubbers; (ix) diapers: ‘Lucky Luke’ dermatitis
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
16. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
(sensitization to rubber components → affection of outer buttocks and hips, similar to a
cowboy’s gun belt holsters), miliaria-like rash under the stickers; (ix) toys: especially toy-
cosmetic products (lipstick, eye shadow).
• Diagnosis: patch tests (gold standard) → (i) include all relevant allergens according to the
clinical history; (ii) patch tests with topical corticosteroids should be read at ≥7 days; (iii)
negative patch test results do not fully exclude ACD.
• Treatment: (i) topical steroids (be careful with sensitization); (ii) emollients (be careful with
sensitization); (iii) allergen avoidance (be careful with product’s labeling).
• Prevention: (i) avoid repetitive contact with potential allergens (eg. nickel-containing jewelries,
PPD-containing tattoos); (ii) promote laws about product’s manufacturing (eg. nickel content in
jewelries); (iii) correct skin barrier in patients with atopic dermatitis; (iv) improve labeling of
manufactured products (eg. cosmetics).
• Causes of perioral dermatitis in children: (i) atopic dermatitis; (ii) lip licking; (iii) inhaled steroids;
(iv) ACD: sunscreens with a high protection factor, toothpaste, dental fillings, chewing gum.
• Products that may contain or cross-react with PPD: hair dyes, azo dyes, tattoos, sulfonamides,
p-aminobenzoic acid sunscreens, benzocaine, procaine.
• BARLEY’S LIPID TRANSFER PROTEIN: A NEW EMERGING ALLERGEN IN PEDIATRIC
ANAPHYLAXIS (Nemni A, Borges JP, Rougé P, Barre A, Just J. Pediatr Allergy Immunol 2013: 24:
410–411):
• Barley (Hordeum vulgare): grain commonly used in alcoholic beverages (mostly whiskey and
beer); most important allergens: prolamins (Hor v 21), alpha- or beta-amylase inhibitors (Hor v
15, Hor v 16, Hor v 17), lipid transfer protein (LTP, Hor v 14); amylase inhibitors are involved in
baker’s asthma induced by barley flour; LTP is involved in allergy to barley’s beer.
• Authors report the case of a 7-yr-old girl with barley allergy → (i) clinical history: anaphylactic
shock after ingestion of cow’s milk and cereals containing barley malt; (ii) laboratory: negative
SPT and sIgE to cow’s milk, wheat, corn, lupine, buckwheat, honey, rye, oat and soy; positive
SPT to barley (3 mm to pearl barley, 2 mm to a heavily malted beer, 10 mm to purified barley
and barley malt); positive sIgE to barley (1.47 kU/l); SDS-PAGE immunoblotting and mass
spectrometry revealed IgE binding to barley’s LTP.
• Author’s commentaries: (i) 1st
reported case of barley allergy in children; (ii) barley is frequently
used in the agro-food chain → avoidance is difficult because of hidden barley allergens in
‘cereal-based’ products; (iii) consider barley’s LTP allergy in children with severe reactions to
cereals → allergen tests should include barley and/or barley’s LTP.
• COMPARISON BETWEEN TWO MAINTENANCE FEEDING REGIMENS AFTER SUCCESSFUL
COW’S MILK ORAL DESENSITIZATION (Pajno GB, Caminiti L, Salzano G, Crisafulli G, Aversa T,
Messina MF, Wasniewska M, Passalacqua G. Pediatr Allergy Immunol 2013: 24: 376–381):
• Cow’s milk allergy: 2-3% of infants; often resolves at childhood or adolescence; conventional
therapy: avoidance (↓ QoL, does not prevent accidental exposure), epinephrine autoinjectors;
novel treatment: oral immunotherapy (main risk: adverse effects).
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
17. The purpose of this summary is exclusively educational, to provide practical updated knowledge for Allergy/Immunology Physicians. It does not
intend to replace the clinical criteria of the physician.
• After desensitization to cow’s milk, it has been recommended a daily intake to maintain
tolerance. Limitation: this maintenance regimen is not suitable for some families.
• Authors performed a RCT in 32 children (4–13 yrs old) after successful desensitization to cow’s
milk (IgE-mediated allergy) → group A (n=16) received 150–200 ml of cow’s milk twice a week;
group B (n=16) received 150–200 ml daily → both maintenance regimens (follow up: 1 yr) had
similar efficacy and safety; most adverse reactions occurred during concurrent exercise or
infection.
• CONSIDERING 25 YEARS OF RESEARCH ON ALLERGY PREVENTION – HAVE WE LET
OURSELVES DOWN? (Whan U. Pediatr Allergy Immunol 2013: 24: 308–310):
• How to prevent allergy initiation? Most proposals over the last 25 yrs have failed or remain
controversial: (i) early or late food introduction in infants; (ii) extension of breastfeeding over the
first 4 months; (iii) ↓ exposure to indoor allergens; (iv) use of bacterial products.
• How to prevent asthma initiation? Most proposals have failed: (i) antihistamines (cetirizine,
levocetirizine and desloratadine) in patients with atopic dermatitis; (ii) pimecrolimus in patients
with atopic dermatitis; (iii) inhaled corticosteroids in children with recurrent wheeze.
• Current recommendations: (i) exclusive breastfeeding for at least 4 months; (ii) use hydrolyzed
formulas in high-risk infants when breastfeeding is not available (may ↓ cow’s milk allergy; may
↓ atopic dermatitis); (iii) supplement prebiotic oligosaccharides to infant formulas; (iv) avoid
tobacco smoke during pregnancy and infancy; (v) consider specific allergen immunotherapy to
prevent allergy progression.
• How to improve research on allergy prevention? Focusing on specific allergic diseases (eg.
targeting respiratory viruses [RSV, rhinovirus] and allergens to prevent allergic asthma).
• IS THERE AN ASSOCIATION BETWEEN MICROBIAL EXPOSURE AND FOOD ALLERGY? A
SYSTEMATIC REVIEW (Marrs T, Bruce KD, Logan K, Rivett DW, Perkin MR, Lack G, Flohr C.
Pediatr Allergy Immunol 2013: 24: 311–320):
• Factors that may ↓ risk of atopic dermatitis and asthma: older siblings, natural delivery, farm
exposure, certain infections, attending childcare, tolerogenic microbiota, prebiotics, probiotics.
• Food allergy burden has ↑ worldwide; origin may involve genetic and environmental factors.
• Authors performed a systematic review of 46 studies to identify the association between food
allergy and several factors that likely influence microbial exposure → (i) study heterogeneity
precluded metaanalysis; (ii) factors that showed association with ↑ food allergy: Cesarean
delivery; (iii) factors that showed association with ↓ food allergy: having siblings, attending
childcare, farm exposure, endotoxin exposure, certain infections, probiotic use, tolerogenic
microbiota; (iv) further prospective studies using DBPCFCs as an outcome are required.
• ROLE OF SPECIFIC IGE IN PREDICTING THE CLINICAL COURSE OF LENTIL ALLERGY IN
CHILDREN (Yavuz ST, Sahiner UM, Buyuktiryaki B, Tuncer A, Yilmaz EA, Cavkaytar O, Karabulut
E, Sackesen C. Pediatr Allergy Immunol 2013: 24: 382–388):
• Lentil (Lens culinaris): legume; important protein source in many countries; lentil seeds are a
frequent cause of IgE-mediated allergy in several countries.
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013
18. Juan Carlos Aldave Becerra, MD
Allergy and Clinical Immunology
Rebagliati Martins National Hospital, Lima-Peru
• Authors studied 30 children (1.3-16.1 yrs old) with IgE-mediated lentil allergy (diagnosis:
consistent clinical history + positive SPT and/or sIgE + positive open OFC in some patients; the
best cutoff level of sIgE to predict clinical reactivity was 4.8 kU/l [sensitivity: 50%, specificity:
100%, PPV: 100%, NPV: 56%] → (i) median age at onset of symptoms: 1.5 yrs; (ii) most
frequent symptoms: cutaneous (97%), respiratory (30%); (iii) anaphylaxis was reported in 27%
of patients; (iv) 4 patients also had symptoms after exposure to steam from cooked lentils; (v)
17% of children were only sensitized to lentils; (vi) most common concomitant food allergies:
chickpea, peanut, pea, sesame, hazelnut, walnut, egg, milk; (vii) 50% of patients outgrew lentil
allergy by 3.5 yrs old; (viii) children with an initial lentil sIgE <4.9 kU/l were more prone to
outgrow allergy; (x) risk of anaphylaxis was remarkably high if lentil sIgE >23 kU/l.
* I suggest revising the journal AllergyWatch, it is a very useful tool to keep updated in the
specialty of Allergy and Clinical Immunology:
http://www.acaai.org/Pages/allergy-watch.aspx
PEARLS IN ALLERGY AND IMMUNOLOGY June 2013