This study compared anterior segment optical coherence tomography (AS-OCT), Scheimpflug imaging, and biomicroscopy for detecting graft detachment after Descemet's membrane endothelial keratoplasty (DMEK) surgery in 120 eyes. AS-OCT detected graft detachment with 64% diagnostic value, higher than Scheimpflug imaging at 36%. Biomicroscopy, Scheimpflug imaging, and AS-OCT all performed equally well at detecting complete graft detachments. However, AS-OCT performed better than Scheimpflug imaging and biomicroscopy for detecting flat or partial graft detachments. The study concluded that AS-OCT is most effective for detecting subtle graft detachments after DMEK surgery