Cytotoxic Biosafety Cabinet Operating Sequence
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Learn the proper procedures and steps to take when working in a Cytotoxic Class II, Type A2 Biological Safety Cabinet from start up to shutdown.
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Biological Safety Cabinet Operating Sequence
A simple and easy presentation on the proper sequence of events a user should take when operating a Biological Safety Cabinet from start up to shutdown.
ARBEITEN AN DER NU-481 SICHERHEITSWERKBANK - BETRIEBSABLAUF
Lernen Sie die richtigen Verfahren und Schritte zu ergreifen, wenn man in einem zytotoxischen Klasse II, Typ A2 starten Biologische Sicherheitswerkbänke aus bis zur Abschaltung.
Tips for Working Safely in your Biosafety Cabinet
This document provides tips for safely working in a biological safety cabinet. Some key points include:
- Know how the airflow works to provide proper protection and avoid disrupting the air curtain.
- Work at the proper sash height and never cover the air grill to maintain airflow integrity.
- Minimize rapid movements and other activities that could disrupt the protective air barrier.
- Follow good techniques to reduce splatters and aerosols and minimize cross-contamination risks.
Transition metal complexes of S N O donors
Transition metal complexes were developed from novel mono/bidentate SNO donor ligands using new synthetic strategies. These complexes were studied for their ligand behavior and structures. Transition metal complexes of quinoline-based thiosemicarbazone ligands were proven to be good antimicrobial agents, while related quinoxaline and triazole complexes exhibited promising anticonvulsant activity. Complexes derived from quinoxaline-thiosemicarbazone ligands also showed significant antidiabetic activity in diabetes-induced rats. Additionally, novel pyrazole-bridged binuclear transition metal complexes were developed that exhibit strong intercalating interaction with E. coli DNA.
Influence of Ethanolic Extractives of Leaves of Mulberry, Morus Alba (L) On 7...
The study deals with investigation of thechemopreventive potential and antilipidperoxidative effects
of ethanolic leaf extract of mulberry, Morus alba (L) (TpEt) on 7,12-dimethylbenz(a)anthracene (DMBA)-
induced buccal pouch carcinoma in Syrian hamster, Mesocricetusauratus (L). Oral squamous cell carcinoma
was developed in the buccal pouch of Syrian golden hamsters, by painting with 0.5% DMBA in liquid paraffin,
thrice a week, for 14 weeks. The tumor incidence, volume and burden were determined. Oral administration
ofTpEt at a dose of 300 mg/kg, body weight, to DMBA (on alternate days for 14 weeks)- painted animals
significantly prevented the incidence, volume and burden of the tumor. TpEt showed potent antilipidperoxidative
effect, as well as enhanced the antioxidant status in DMBA- painted animals. TpEt has potent chemopreventive
efficacy and significant antilipidperoxidative effect, in DMBA-induced oral carcinogenesis. The active principle
of mulberry leaf may have the abilities of induction of apoptosis, which involve disruption of mitochondrial
membrane potential, release of cytochrome C and activation of caspase. With it’s bioactive compounds,
mulberry, Morus alba (L) may open a new avenue in the cancer prevention and treatment.
IN VITRO EVALUATION OF CYTOTOXIC ACTIVITY OF HYDRAZIDEHYDRAZONES BASED ON 4-C...
Two new coumarin-based hydrazide/hydrazones were synthesized and evaluated for their cytotoxicity against two chronic lymphocytic leukemia cell lines. Both compounds showed strong cytotoxicity, with IC50 values several times lower than the reference drug bendamustine. Exposure to the compounds induced fragmentation of leukemic cell nuclei, a hallmark of apoptosis. Quantification assays further confirmed the compounds induced apoptosis in the leukemia cells. The results indicate the new hydrazide/hydrazones have more potent antileukemic effects in vitro compared to bendamustine.
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This document provides information on safe administration and preparation of cancer chemotherapy. It discusses the hazards of anticancer medicines and outlines various safety considerations for personnel, patients, and the environment. Specific guidelines are presented for reconstitution, administration, storage, spill management, oral drug handling, and waste disposal. The importance of patient safety is emphasized, and factors to consider for individual patients are described. Medication errors can occur at various stages, so communication and information sharing need to be standardized. Overall, strict protocols and protective measures are necessary when working with hazardous chemotherapy drugs.
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Biological Safety Cabinet Operating Sequence
A simple and easy presentation on the proper sequence of events a user should take when operating a Biological Safety Cabinet from start up to shutdown.
ARBEITEN AN DER NU-481 SICHERHEITSWERKBANK - BETRIEBSABLAUF
Lernen Sie die richtigen Verfahren und Schritte zu ergreifen, wenn man in einem zytotoxischen Klasse II, Typ A2 starten Biologische Sicherheitswerkbänke aus bis zur Abschaltung.
Tips for Working Safely in your Biosafety Cabinet
This document provides tips for safely working in a biological safety cabinet. Some key points include:
- Know how the airflow works to provide proper protection and avoid disrupting the air curtain.
- Work at the proper sash height and never cover the air grill to maintain airflow integrity.
- Minimize rapid movements and other activities that could disrupt the protective air barrier.
- Follow good techniques to reduce splatters and aerosols and minimize cross-contamination risks.
Transition metal complexes of S N O donors
Transition metal complexes were developed from novel mono/bidentate SNO donor ligands using new synthetic strategies. These complexes were studied for their ligand behavior and structures. Transition metal complexes of quinoline-based thiosemicarbazone ligands were proven to be good antimicrobial agents, while related quinoxaline and triazole complexes exhibited promising anticonvulsant activity. Complexes derived from quinoxaline-thiosemicarbazone ligands also showed significant antidiabetic activity in diabetes-induced rats. Additionally, novel pyrazole-bridged binuclear transition metal complexes were developed that exhibit strong intercalating interaction with E. coli DNA.
Influence of Ethanolic Extractives of Leaves of Mulberry, Morus Alba (L) On 7...
The study deals with investigation of thechemopreventive potential and antilipidperoxidative effects
of ethanolic leaf extract of mulberry, Morus alba (L) (TpEt) on 7,12-dimethylbenz(a)anthracene (DMBA)-
induced buccal pouch carcinoma in Syrian hamster, Mesocricetusauratus (L). Oral squamous cell carcinoma
was developed in the buccal pouch of Syrian golden hamsters, by painting with 0.5% DMBA in liquid paraffin,
thrice a week, for 14 weeks. The tumor incidence, volume and burden were determined. Oral administration
ofTpEt at a dose of 300 mg/kg, body weight, to DMBA (on alternate days for 14 weeks)- painted animals
significantly prevented the incidence, volume and burden of the tumor. TpEt showed potent antilipidperoxidative
effect, as well as enhanced the antioxidant status in DMBA- painted animals. TpEt has potent chemopreventive
efficacy and significant antilipidperoxidative effect, in DMBA-induced oral carcinogenesis. The active principle
of mulberry leaf may have the abilities of induction of apoptosis, which involve disruption of mitochondrial
membrane potential, release of cytochrome C and activation of caspase. With it’s bioactive compounds,
mulberry, Morus alba (L) may open a new avenue in the cancer prevention and treatment.
IN VITRO EVALUATION OF CYTOTOXIC ACTIVITY OF HYDRAZIDEHYDRAZONES BASED ON 4-C...
Two new coumarin-based hydrazide/hydrazones were synthesized and evaluated for their cytotoxicity against two chronic lymphocytic leukemia cell lines. Both compounds showed strong cytotoxicity, with IC50 values several times lower than the reference drug bendamustine. Exposure to the compounds induced fragmentation of leukemic cell nuclei, a hallmark of apoptosis. Quantification assays further confirmed the compounds induced apoptosis in the leukemia cells. The results indicate the new hydrazide/hydrazones have more potent antileukemic effects in vitro compared to bendamustine.
Imidazole Derivatives Biological Activity And Synthetic Approaches
This document summarizes biological activity and synthetic approaches for imidazole derivatives. It discusses the biological importance of natural imidazoles and synthetic imidazoles used as drugs, including antifungals, antithyroid drugs, and drugs affecting the sympathetic nervous system. It also reviews conventional imidazole synthesis methods and their limitations, and modern catalytic and non-catalytic methods that offer better yields, selectivity, and green chemistry profiles.
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This document provides information on safe administration and preparation of cancer chemotherapy. It discusses the hazards of anticancer medicines and outlines various safety considerations for personnel, patients, and the environment. Specific guidelines are presented for reconstitution, administration, storage, spill management, oral drug handling, and waste disposal. The importance of patient safety is emphasized, and factors to consider for individual patients are described. Medication errors can occur at various stages, so communication and information sharing need to be standardized. Overall, strict protocols and protective measures are necessary when working with hazardous chemotherapy drugs.
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1. Infrared spectroscopy can be used to qualitatively and quantitatively analyze compounds. It is used to identify unknown substances by comparing their IR spectra to reference standards.
2. The "fingerprint" region from 1200-700 cm-1 is particularly useful for identification because small molecular differences result in significant spectral changes in this region. Computer search systems can also identify compounds by matching IR spectra to profiles of pure compounds.
3. IR spectroscopy allows determination of molecular structures by identifying the presence or absence of functional groups from their characteristic absorption bands. It can also be used to study the progress of chemical reactions.
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Mass spectroscopy is a technique used to analyze molecules. It involves ionizing molecules using electrons, accelerating the ions, and separating them based on their mass-to-charge ratio using electric or magnetic fields. The ions are then detected, producing a mass spectrum that is unique to each molecule and can be used to determine molecular structure. Mass spectroscopy requires only a small amount of sample and provides accurate molecular mass and elemental composition information. It is a destructive technique as the sample is consumed during ionization and fragmentation processes.
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C NMR spectrum. Decoupling and DEPT experiments can also provide information about the types of carbon atoms present.
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Diagnosis and Staging
The diagnosis of HR+ breast cancer begins with clinical evaluation, imaging, and biopsy. Imaging modalities such as mammography, ultrasound, and MRI help in assessing the extent of the disease. Histopathological examination and immunohistochemical staining of the biopsy sample confirm the diagnosis and hormone receptor status by identifying the presence of estrogen receptors (ER) and progesterone receptors (PR) on the tumor cells.
Staging involves determining the size of the tumor (T), the involvement of regional lymph nodes (N), and the presence of distant metastasis (M). The American Joint Committee on Cancer (AJCC) staging system is commonly used. Accurate staging is critical as it guides treatment decisions.
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Endocrine Therapy
Endocrine therapy is the cornerstone of treatment for HR+ breast cancer in postmenopausal women. The primary goal is to reduce the levels of estrogen or block its effects on cancer cells. Commonly used agents include:
Selective Estrogen Receptor Modulators (SERMs): Tamoxifen is a SERM that binds to estrogen receptors, blocking estrogen from stimulating breast cancer cells. It is effective but may have side effects such as increased risk of endometrial cancer and thromboembolic events.
Aromatase Inhibitors (AIs): These drugs, including anastrozole, letrozole, and exemestane, lower estrogen levels by inhibiting the aromatase enzyme, which converts androgens to estrogen in peripheral tissues. AIs are generally preferred in postmenopausal women due to their efficacy and safety profile compared to tamoxifen.
Selective Estrogen Receptor Downregulators (SERDs): Fulvestrant is a SERD that degrades estrogen receptors and is used in cases where resistance to other endocrine therapies develops.
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Combining endocrine therapy with other treatments enhances efficacy. Examples include:
Endocrine Therapy with CDK4/6 Inhibitors: Palbociclib, ribociclib, and abemaciclib are CDK4/6 inhibitors that, when combined with endocrine therapy, significantly improve progression-free survival in advanced HR+ breast cancer.
Endocrine Therapy with mTOR Inhibitors: Everolimus, an mTOR inhibitor, can be added to endocrine therapy for patients who have developed resistance to aromatase inhibitors.
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One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells.
Safe methods have been devised to do this, using several viral and non-viral vectors.
In the future, this technique may allow doctors to treat a disorder by inserting a gene into a patient's cells instead of using drugs or surgery.
The biggest hurdle faced by medical research in gene therapy is the availability of effective gene-carrying vectors that meet all of the following criteria:
Protection of transgene or genetic cargo from degradative action of systemic and endonucleases,
Delivery of genetic material to the target site, i.e., either cell cytoplasm or nucleus,
Low potential of triggering unwanted immune responses or genotoxicity,
Economical and feasible availability for patients .
Viruses are naturally evolved vehicles that efficiently transfer their genes into host cells.
Choice of viral vector is dependent on gene transfer efficiency, capacity to carry foreign genes, toxicity, stability, immune responses towards viral antigens and potential viral recombination.
There are a wide variety of vectors used to deliver DNA or oligo nucleotides into mammalian cells, either in vitro or in vivo.
The most common vector system based on retroviruses, adenoviruses, herpes simplex viruses, adeno associated viruses.
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It is mostly found in the brain, intestines, and blood platelets.
5-HT is utilised to transport messages between nerve cells, is known to be involved in smooth muscle contraction, and adds to overall well-being and pleasure, among other benefits. 5-HT regulates the body's sleep-wake cycles and internal clock by acting as a precursor to melatonin.
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Adsorption chromatography is a type of chromatography where the mobile phase is adsorbed onto the surface of a stationary solid phase. Separation occurs due to differences in how strongly components compete for active sites on the adsorbent, based on electrostatic forces. Common adsorbents include silica gel and alumina. Thin layer chromatography is a technique where the mobile phase flows over a thin layer of adsorbent on a plate, separating components by differential migration.
Raman spectroscopy
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Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
1. Infrared spectroscopy can be used to qualitatively and quantitatively analyze compounds. It is used to identify unknown substances by comparing their IR spectra to reference standards.
2. The "fingerprint" region from 1200-700 cm-1 is particularly useful for identification because small molecular differences result in significant spectral changes in this region. Computer search systems can also identify compounds by matching IR spectra to profiles of pure compounds.
3. IR spectroscopy allows determination of molecular structures by identifying the presence or absence of functional groups from their characteristic absorption bands. It can also be used to study the progress of chemical reactions.
Mass Spectroscopy
Mass spectroscopy is a technique used to analyze molecules. It involves ionizing molecules using electrons, accelerating the ions, and separating them based on their mass-to-charge ratio using electric or magnetic fields. The ions are then detected, producing a mass spectrum that is unique to each molecule and can be used to determine molecular structure. Mass spectroscopy requires only a small amount of sample and provides accurate molecular mass and elemental composition information. It is a destructive technique as the sample is consumed during ionization and fragmentation processes.
C 13 NMR Spectroscopy
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C NMR spectroscopy provides information about the number and types of nonequivalent carbon atoms in a molecule. It detects the number of protons bonded to each carbon and the electronic environment of the carbons. The chemical shift range for 1313
C NMR is much wider than for 1H NMR, from 0 to 220 ppm versus 0 to 12 ppm, making individual carbon signals easier to distinguish. Signal averaging and Fourier transform techniques improve the sensitivity of the 1313
C NMR spectrum. Decoupling and DEPT experiments can also provide information about the types of carbon atoms present.
Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance Spectroscopy is a technique used to characterize organic molecules by identifying carbon-hydrogen frameworks. It exploits the magnetic properties of atomic nuclei when subjected to radio waves and magnetic fields. There are two main types of NMR spectroscopy: 1H NMR determines the number and type of hydrogen atoms, and 13C NMR determines the type of carbon atoms. When nuclei are placed in a magnetic field, their spins can be aligned with or against the field, producing detectable signals. Chemical shifts in these signals provide information about the molecular structure and atomic environment of the nuclei.
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This document outlines a PowerPoint presentation on nuclear magnetic resonance (NMR) spectroscopy. It covers the fundamentals of NMR including spin-spin coupling, instrumentation, solvents, chemical shifts, and 2D NMR techniques. Applications discussed include structure elucidation of organic compounds and biomolecules, as well as clinical uses such as MRI. Specific NMR experiments summarized are COSY, NOESY, and HETCOR.
Principles and application of chromatography
Chromatography is a laboratory technique used to separate mixtures by distributing components to separate between two phases, one stationary and one mobile. It works based on how substances partition between the phases and move through the system at different rates. Common techniques include column chromatography, thin layer chromatography, gas chromatography, and high performance liquid chromatography. Chromatography is used in analytical chemistry to identify unknown substances and quantify components in a mixture.
UV visible spectroscopy
UV/visible spectroscopy involves the interaction of electromagnetic radiation with matter. Absorption spectroscopy measures the absorption of UV or visible light, while emission spectroscopy measures light emitted from a sample. The wavelength and frequency of electromagnetic radiation are inversely related by the equation c=λν. Electronic transitions in molecules, such as σ→σ*, π→π*, n→σ*, and n→π* can be detected using UV/visible spectroscopy. Beer's law states that absorbance is directly proportional to concentration and path length. Chromophores are functional groups in molecules that absorb UV or visible light.
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Paper chromatography is a technique introduced in 1865 that separates mixtures by analyzing how compounds partition between a stationary phase (cellulose paper) and a mobile phase (solvent). It is simple and widely used. Compounds are separated based on how they partition between the water in cellulose fibers and an organic solvent. Samples are applied to a line on paper and developed in a chamber saturated with solvent vapors. Compounds form separate spots visualized with detectors like iodine or UV light. Quantification is done by comparing spot intensities to a standard.
Introduction and Principle of IR spectroscopy
This document provides an introduction to infrared (IR) spectrophotometry. It discusses how IR spectroscopy analyzes molecular vibrations when molecules absorb IR radiation that matches their natural vibrational frequencies. The document outlines the principle of IR spectroscopy and describes the different types of molecular vibrations observed in IR spectra, including stretching and bending vibrations. It also discusses the criteria for a molecule to absorb IR radiation, such as having a change in dipole moment when vibrations occur.
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Column chromatography is a separation technique that uses a stationary phase, usually a solid, and a mobile liquid phase to separate mixtures. It was developed in 1900 and involves passing a liquid containing dissolved compounds through a column packed with a solid adsorbent. Components separate based on their different interactions with the stationary and mobile phases, with less strongly adsorbed compounds eluting more quickly. Column chromatography is useful for purifying compounds and isolating constituents from mixtures.
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The development of nanogold-based cancer therapy could revolutionize oncology by providing a more targeted, less invasive treatment option. This project contributes to the growing body of research aimed at harnessing nanotechnology for medical applications, paving the way for future clinical trials and potential commercial applications.
Cancer remains one of the leading causes of death worldwide, prompting the need for innovative treatment methods. Nanotechnology offers promising new approaches, including the use of gold nanoparticles (nanogold) for targeted cancer therapy. Nanogold particles possess unique physical and chemical properties that make them suitable for drug delivery, imaging, and photothermal therapy.
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Pictorial and detailed description of patellar instability with sign and symptoms and how to diagnose , what investigations you should go with and how to approach with treatment options . I have presented this slide in my 2nd year junior residency in orthopedics at LLRM medical college Meerut and got good reviews for it
After getting it read you will definitely understand the topic.
Breast cancer: Post menopausal endocrine therapy
Breast cancer in postmenopausal women with hormone receptor-positive (HR+) status is a common and complex condition that necessitates a multifaceted approach to management. HR+ breast cancer means that the cancer cells grow in response to hormones such as estrogen and progesterone. This subtype is prevalent among postmenopausal women and typically exhibits a more indolent course compared to other forms of breast cancer, which allows for a variety of treatment options.
Diagnosis and Staging
The diagnosis of HR+ breast cancer begins with clinical evaluation, imaging, and biopsy. Imaging modalities such as mammography, ultrasound, and MRI help in assessing the extent of the disease. Histopathological examination and immunohistochemical staining of the biopsy sample confirm the diagnosis and hormone receptor status by identifying the presence of estrogen receptors (ER) and progesterone receptors (PR) on the tumor cells.
Staging involves determining the size of the tumor (T), the involvement of regional lymph nodes (N), and the presence of distant metastasis (M). The American Joint Committee on Cancer (AJCC) staging system is commonly used. Accurate staging is critical as it guides treatment decisions.
Treatment Options
Endocrine Therapy
Endocrine therapy is the cornerstone of treatment for HR+ breast cancer in postmenopausal women. The primary goal is to reduce the levels of estrogen or block its effects on cancer cells. Commonly used agents include:
Selective Estrogen Receptor Modulators (SERMs): Tamoxifen is a SERM that binds to estrogen receptors, blocking estrogen from stimulating breast cancer cells. It is effective but may have side effects such as increased risk of endometrial cancer and thromboembolic events.
Aromatase Inhibitors (AIs): These drugs, including anastrozole, letrozole, and exemestane, lower estrogen levels by inhibiting the aromatase enzyme, which converts androgens to estrogen in peripheral tissues. AIs are generally preferred in postmenopausal women due to their efficacy and safety profile compared to tamoxifen.
Selective Estrogen Receptor Downregulators (SERDs): Fulvestrant is a SERD that degrades estrogen receptors and is used in cases where resistance to other endocrine therapies develops.
Combination Therapies
Combining endocrine therapy with other treatments enhances efficacy. Examples include:
Endocrine Therapy with CDK4/6 Inhibitors: Palbociclib, ribociclib, and abemaciclib are CDK4/6 inhibitors that, when combined with endocrine therapy, significantly improve progression-free survival in advanced HR+ breast cancer.
Endocrine Therapy with mTOR Inhibitors: Everolimus, an mTOR inhibitor, can be added to endocrine therapy for patients who have developed resistance to aromatase inhibitors.
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Chemotherapy is generally reserved for patients with high-risk features, such as large tumor size, high-grade histology, or extensive lymph node involvement. Regimens often include anthracyclines and taxanes.
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Gene therapy can be broadly defined as the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient.
One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells.
Safe methods have been devised to do this, using several viral and non-viral vectors.
In the future, this technique may allow doctors to treat a disorder by inserting a gene into a patient's cells instead of using drugs or surgery.
The biggest hurdle faced by medical research in gene therapy is the availability of effective gene-carrying vectors that meet all of the following criteria:
Protection of transgene or genetic cargo from degradative action of systemic and endonucleases,
Delivery of genetic material to the target site, i.e., either cell cytoplasm or nucleus,
Low potential of triggering unwanted immune responses or genotoxicity,
Economical and feasible availability for patients .
Viruses are naturally evolved vehicles that efficiently transfer their genes into host cells.
Choice of viral vector is dependent on gene transfer efficiency, capacity to carry foreign genes, toxicity, stability, immune responses towards viral antigens and potential viral recombination.
There are a wide variety of vectors used to deliver DNA or oligo nucleotides into mammalian cells, either in vitro or in vivo.
The most common vector system based on retroviruses, adenoviruses, herpes simplex viruses, adeno associated viruses.
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5-hydroxytryptamine or 5-HT or Serotonin is a neurotransmitter that serves a range of roles in the human body. It is sometimes referred to as the happy chemical since it promotes overall well-being and happiness.
It is mostly found in the brain, intestines, and blood platelets.
5-HT is utilised to transport messages between nerve cells, is known to be involved in smooth muscle contraction, and adds to overall well-being and pleasure, among other benefits. 5-HT regulates the body's sleep-wake cycles and internal clock by acting as a precursor to melatonin.
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Cytotoxic Biosafety Cabinet Operating Sequence
- 1. Cytotoxic Biological Safety Cabinet Operating Sequence NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A. | www.nuaire.com
- 2. Sequence Overview 1. Start Up 2. Wipedown 3. Materials and Equipment 4. Air Purge 5. Perform Work 6. Terminal Purging and Wipedown 7. Paper Catch/Prefilter 8. Shutdown
- 3. 1) Start Up • Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make sure they are unobstructed. • Allow blower to operate for a minimum of 15 minutes before aseptic manipulations are begun in the cabinet.
- 4. 2) Wipedown • Disinfect all interior surfaces of the workspace 70% alcohol or similar non-corrosive anti-microbial agents. Use of chlorinated or halogen materials in the cabinet may damage stainless steel. If present, re-wipe with 70% Alcohol to remove damaging stainless steel materials.
- 5. 3) Materials and Equipment • Place apparatus and materials onto the work surface. • Do not place any items over the front or rear intake grills. • Separate contaminated materials on the work surface to minimize movement. • Do not use constant flame Bunsen burners.
- 6. 4) Air Purge • Allow 2-3 minutes after apparatus and materials are placed into the cabinet.
- 7. 5) Perform Work • Use proper attire, long sleeve gown, knit cuffs and appropriate gloves for handling of cytotoxic materials. • Minimize movement and use proper aseptic technique. • Cytotoxic preparation should observe the following: a. A sterile plastic-backed absorbent drape should be placed on the work surface during mixing procedures. The drape should be exchanged whenever significant spillage occurs, or at the end of each production sequence.
- 8. 5) Perform Work (continued) b. Vials should be vented with a filter needle to eliminate internal pressure or vacuum. c. Before opening ampoules, care should be taken to insure that no liquid remains in the tip of the ampoule. A sterile gauze sponge should be wrapped around the neck of the ampoule while opening. d. Final drug measurement should be performed prior to removing the needle from the stopper of the vial. e. A non-splash collection vessel should be available in the work zone to discard excess drug solutions.
- 9. 5) Perform Work (continued) • If an alarm occurs during the work process, immediately stop working, evaluate the alarm type on the control panel. If related to airflow, immediately cover and/or stop work processes, identify the work materials at risk during the work process. Proceed to terminal purging (if possible), removal of materials and interior wipe down.
- 10. 6) Terminal Purging & Wipedown • Allow 2-3 minutes before removal of material and apparatus from cabinet. • Remove residual cytotoxic materials from all interior surfaces using a light aqueous sodium hydroxide solution (0.05M NaOH for daily use, 1M for weekly cleaning). Re-wipe all interior surfaces of the workspace 70% alcohol or similar non-corrosive antimicrobial agents. Use of chlorinated or halogen materials in the cabinet may damage stainless steel. If present, re-wipe with 70% Alcohol to remove damaging stainless steel materials.
- 11. 7) Paper Catch/Prefilter • A paper catch is installed under the work surface and should be checked and cleaned no less than on a weekly basis. Any paper removed must be removed and properly disposed of as contaminated hazardous waste.
- 12. 8) Shut Down • Turn off blower and lights. Do not use the cabinet for storage!
- 13. NuAire • NuAire is a manufacturer of Biological Safety Cabinets and Laboratory Equipment • For questions or additional information please visit www.nuaire.com or contact us via email nuaire@nuaire.com