Zika virus - Dr. S. Sivasankara Narayani

1. ZIKA VIRUS
Dr. S. SIVASANKARA NARAYANI
06-08-2020Dr.SS

2. ZIKA
• Genus Flavivirus ; Family Flaviviridae
• Transmitted by Mosquito (Aedes aegypti)
• First identified in 1947 in Zika forest of Uganda from Rhesus monkeys
• In 1948, isolated from mosquitoes
• In 1952, isolated from humans
06-08-2020Dr.SS

3. ZIKA – AFFECTED COUNTRIES
• Pre 2015 – Africa, southeast Asia, Pacific islands
• May 2015 –Pan American Health Organization – Brazil
• Feb 2016 – WHO –Public Health Emergency of International Concern (PHEIC)
• July 2016 –Florida Public Health –Florida
• NoV 2016 – Texas Public Health – Texas
06-08-2020Dr.SS

4. STRUCTURE
• RNA – genetic material
• Spherical and small – 50nm in diameter
• Genome Size – 10.8KB
Structural Proteins
• Capsid (c)
• Membrane (M)
• Precursor premembrane (PrM)
• Envelope (E)
Non Structural Proteins
• NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5
06-08-2020Dr.SS

5. 06-08-2020Dr.SS
Life cycle

6. 06-08-2020Dr.SS
Genes
involved

7. 06-08-2020Dr.SS
Zika
Virus
infection

8. DIAGNOSIS
• Sample
Blood,SalivaandUrine
• PCR
Itisusefulin3-5daysafteronsetofsymptoms.
DetectionofZikavirusRNA
• Serology
Detectthepresenceofantibodies-IgM
06-08-2020Dr.SS

9. TREATMENT
• No specific vaccine
• Rest
• Drink fluid
• Acetaminophen –to reduce fever and pain
06-08-2020Dr.SS

10. TREATMENT
• Emricasan targets caspase-3 activity to prevent cell death.
• Z2 peptide targets ZIKV cell entry.
• 25-hydroxycholesterol and chloroquine interfere with lipid homeostasis and autophagy to disrupt viral particle release
after endocytosis.
• Temoporfin, nitazoxanide, niclosamide, and viperin block NS2B/NS3 protease activity to prevent viral replication.
• Sofosbuvir, merimepodib, N-(4-hydrophenyl) retinamide, 7-deaza-2′-C-methyladenosine, NITD008, BCX4430, and
ribavarin block ZIKV NS5 polymerase activity to prevent viral replication.
• Ribavarin and merimepodib block IMPDH, an enzyme involved in de novo synthesis of guanine nucleotides.
• Niclosamide, EGCG, and cavinafungin block CDKs and prevent viral replication.
06-08-2020Dr.SS

11. CLARIFICATION
https://www.frontiersin.org/articles/10.3389/fmicb.2018.03252/full
06-08-2020Dr.SS
sivasan91@gmail.com

12. QUESTIONS TO THINK
• Role of Non structural proteins
• In what way able to stop Life cycle of zikavirus?
• What is microcephaly?
• What is Gullain Barr syndrome?
• ADE by DENV antibodies?
• What is the main function of RdRP?
06-08-2020Dr.SS

13. 06-08-2020Dr.SS