A 50 mL stock sample of cells is diluted 110 six times. If 0.1 mL o.pdf

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A 50 mL stock sample of cells is diluted 1/10 six times. If 0.1 mL of the most dilute sample generates 75 colonies on an agar plate, calculate the original concentration of bacteria present in the original sample? Solution Answer: Given: Dilution factor= 1/10^6 Number of colonies= 75 Volume of sample = 0.1 mL to the plate CFU/ml = ? cfu/ml = (no. of colonies)/ dilution factor x volume of culture plate cfu/ml = (75)/ (1/10^6 x 0.1) cfu/ml = 75 x 10^7.

A 50 mL stock sample of cells is diluted 1/10 six times. If 0.1 mL of the most dilute sample
generates 75 colonies on an agar plate, calculate the original concentration of bacteria present in
the original sample?
Solution
Answer:
Given:
Dilution factor= 1/10^6
Number of colonies= 75
Volume of sample = 0.1 mL to the plate
CFU/ml = ?
cfu/ml = (no. of colonies)/ dilution factor x volume of culture plate
cfu/ml = (75)/ (1/10^6 x 0.1)
cfu/ml = 75 x 10^7

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