Hippocampal transcriptomic responses to technical and biological perturbations

•

1 like•199 views

A short talk about my research for the Neural Systems & Behavior course.

Hippocampal transcriptomic
responses to technical and
biological perturbations
Rayna M. Harris
Neural Systems & Behavior Course
July 24, 2017

Technological advances now
allow single-cell transcriptomics
Lane K et at. (2017) Measuring Signaling and RNA-
Seq in the Same Cell Links Gene Expression to
Dynamic Patterns of NF-κB Activation. Cell Syst

Challenge: Brain tissues consists
of intricate neural layers & circuits
Brain Tissue
Single Neurons

Research Questions
• How does the process of cellular dissociation alter
gene expression?
• Does the process of cellular dissociation alter our
ability to identify biologically meaningful patterns of
gene expression?

Approach
Up-regulated genes
and pathways
Down-regulated genes
and pathways
Cembrowski et at. (2016) eLife

‘Tissue punch’ sample collection

Reproducible Bioinformatics
https://github.com/raynamharris/DissociationTest

Git for version control and GitHub
for sharing data and analysis
https://www.youtube.com/watch?v=taeAqimxXWo

The effect of cellular dissociation
on hippocampal transcriptomes
Harris RM et at. (2017) BioRxiv

The effect of a stressful experience
on hippocampal transcriptomes
Harris RM et at. (2017) BioRxiv

The effect of a cognitive training
on hippocampal transcriptomes
Harris RM et at. (2017) BioRxiv

Unique and shared responses to
technical and biological perturbations
Harris RM et at. (2017) BioRxiv

Meta analysis of reveals speciﬁc
and robust patterns of expression
Harris RM et at. (2017) BioRxiv

Summary

Thanks!
• Hans Hofmann & all the
Hofmann lab members
• UT Genomic Sequencing &
Analysis Facility
• UT Center for Computational
Biology & Bioinformatics
• Misha Matz & Rachel Wright
• Boris Zemelman, Laura Colgin
• Andre Fenton, Maddy Kao,
Ain Chung, Juan Marcos
Alarcon at NYU & SUNY
Downstate
• The Neural Systems &
Behavior Community
• Software/Data Carpentry
• NSF, NIH, Grass Foundation,
Promega, JAX Lab
15

More Related Content

What's hot

Beiko hpcs

08 Reproductive Sciences

08 Reproductive Sciences

08 Reproductive Sciences

281 lec3 mendel_genetics

281 lec3 mendel_genetics

281 lec3 mendel_genetics

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Lesson 21 present day evolutionary theories

Lesson 21 present day evolutionary theories

Lesson 21 present day evolutionary theories

Genetics of gene expression primer

Genetics of gene expression primer

Genetics of gene expression primer

Functional association networks - The STRING and STITCH web resources

Functional association networks - The STRING and STITCH web resources

Functional association networks - The STRING and STITCH web resources

Lars Juhl Jensen

It is increasingly common to combine Microarray and Quantitative Trait Loci data to aid the search for candidate genes responsible for phenotypic variation. Workflows provide a means of systematically processing these large datasets and also represent a framework for the re-use and the explicit declaration of experimental methods. Here we highlight the issues facing the manual analysis of microarray and QTL data for the discovery of candidate genes underlying complex phenotypes. We show how automated approaches provide a systematic means to investigate genotype-phenotype correlations. This methodology was applied to a use case of resistance to African trypanosomiasis in the mouse. Pathways represented in the results identified Daxx as one of the candidate genes within the Tir1 QTL region.

A systematic approach to Genotype-Phenotype correlations

A systematic approach to Genotype-Phenotype correlations

A systematic approach to Genotype-Phenotype correlations

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay

What's hot (10)

Beiko hpcs

08 Reproductive Sciences

08 Reproductive Sciences

08 Reproductive Sciences

281 lec3 mendel_genetics

281 lec3 mendel_genetics

281 lec3 mendel_genetics

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Rigor Mortis and Intestinal Necrosis During C. elegans Death

Lesson 21 present day evolutionary theories

Lesson 21 present day evolutionary theories

Lesson 21 present day evolutionary theories

Genetics of gene expression primer

Genetics of gene expression primer

Genetics of gene expression primer

Functional association networks - The STRING and STITCH web resources

Functional association networks - The STRING and STITCH web resources

Functional association networks - The STRING and STITCH web resources

A systematic approach to Genotype-Phenotype correlations

A systematic approach to Genotype-Phenotype correlations

A systematic approach to Genotype-Phenotype correlations

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Christopher McKay - Rensselaer Polytechnic Institute - BMES 2014 Oral Present...

Similar to Hippocampal transcriptomic responses to technical and biological perturbations

Systems biology - Understanding biology at the systems level

Systems biology - Understanding biology at the systems level

Systems biology - Understanding biology at the systems level

Lars Juhl Jensen

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

Cross-species data integration

Cross-species data integration

Cross-species data integration

Lars Juhl Jensen

STRING & related databases: Large-scale integration of heterogeneous data

STRING & related databases: Large-scale integration of heterogeneous data

STRING & related databases: Large-scale integration of heterogeneous data

Lars Juhl Jensen

“Organisms function in an integrated manner-our senses, our muscles, our metabolism and our minds work together seamlessly. But biologists have historically studied organisms part by part and celebrated the modern ability to study them molecule by molecule, gene by gene. Systems biology is critical science of future that seeks to understand the integration of the pieces to form biological systems” (David Baltimore, Nobel Laureate)

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

10.1.1.80.2149

5th RNA-Seq San Francisco Agenda

5th RNA-Seq San Francisco Agenda

5th RNA-Seq San Francisco Agenda

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

Trends In Genomics

Trends In Genomics

Trends In Genomics

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

GigaScience, BGI Hong Kong

Network biology: Large-scale integration of data and text

Network biology: Large-scale integration of data and text

Network biology: Large-scale integration of data and text

Lars Juhl Jensen

Protein interaction networks

Protein interaction networks

Protein interaction networks

Lars Juhl Jensen

Cellular Network Biology: Large-scale integration of data and text

Cellular Network Biology: Large-scale integration of data and text

Cellular Network Biology: Large-scale integration of data and text

Lars Juhl Jensen

NAISTビッグデータシンポジウム - バイオ久保先生

NAISTビッグデータシンポジウム - バイオ久保先生

NAISTビッグデータシンポジウム - バイオ久保先生

STRING: protein association networks

STRING: protein association networks

STRING: protein association networks

Lars Juhl Jensen

STRING: Protein association networks

STRING: Protein association networks

STRING: Protein association networks

Lars Juhl Jensen

STRING - Prediction of a functional association network for the yeast mitocho...

STRING - Prediction of a functional association network for the yeast mitocho...

STRING - Prediction of a functional association network for the yeast mitocho...

Lars Juhl Jensen

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Institute for the Awakened Mind

Keynote Talk presented at the 1st Annual BiVi Community Annual Meeting (17 December 2014) http://bivi.co/page/bivi-annual-meeting-16-17th-december-2014 Visualization Approaches for Biomedical Omics Data: Putting It All Together The rapid proliferation of high quality, low cost genome-wide measurement technologies such as whole-genome and transcriptome sequencing, as well as advances in epigenomics and proteomics, are enabling researchers to perform studies that generate heterogeneous datasets for cohorts of thousands of individuals. A common feature of these studies is that a collection of genome-wide, molecular data types and phenotypic or clinical characterizations are available for each individual. These data can be used to identify the molecular basis of diseases and to characterize and describe the variations that are relevant for improved diagnosis, prognosis and targeted treatment of patients. An example for a study in which this approach has been successfully applied is The Cancer Genome Atlas project (http://cancergenome.nih.gov). In my talk I will discuss how visualization approaches can be applied to enable exploration and support analysis of data generated by such studies. Specifically, I will review techniques and tools for visual exploration of individual omics data types, their ability to scale to large numbers of individuals or samples, and emerging techniques that integrate multiple omics data types for interactive visual analysis. I will also examine technical and legal challenges that developers of such visualization tools are facing. To conclude my talk, I will outline research opportunities for the biological data visualization community that address major challenges in this domain.

Visualization Approaches for Biomedical Omics Data: Putting It All Together

Visualization Approaches for Biomedical Omics Data: Putting It All Together

Visualization Approaches for Biomedical Omics Data: Putting It All Together

Nils Gehlenborg

PhDc exam presentation

PhDc exam presentation

PhDc exam presentation

Carlos Manuel Estévez-Bretón Riveros

Similar to Hippocampal transcriptomic responses to technical and biological perturbations (20)

Systems biology - Understanding biology at the systems level

Systems biology - Understanding biology at the systems level

Systems biology - Understanding biology at the systems level

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

scRNA-Seq Lecture - Stem Cell Network RNA-Seq Workshop 2017

Cross-species data integration

Cross-species data integration

Cross-species data integration

STRING & related databases: Large-scale integration of heterogeneous data

STRING & related databases: Large-scale integration of heterogeneous data

STRING & related databases: Large-scale integration of heterogeneous data

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

Interactomics, Integromics to Systems Biology: Next Animal Biotechnology Fron...

10.1.1.80.2149

5th RNA-Seq San Francisco Agenda

5th RNA-Seq San Francisco Agenda

5th RNA-Seq San Francisco Agenda

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

scRNA-Seq Workshop Presentation - Stem Cell Network 2018

Trends In Genomics

Trends In Genomics

Trends In Genomics

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

Lisa Johnson at #ICG13: Re-assembly, quality evaluation, and annotation of 67...

Network biology: Large-scale integration of data and text

Network biology: Large-scale integration of data and text

Network biology: Large-scale integration of data and text

Protein interaction networks

Protein interaction networks

Protein interaction networks

Cellular Network Biology: Large-scale integration of data and text

Cellular Network Biology: Large-scale integration of data and text

Cellular Network Biology: Large-scale integration of data and text

NAISTビッグデータシンポジウム - バイオ久保先生

NAISTビッグデータシンポジウム - バイオ久保先生

NAISTビッグデータシンポジウム - バイオ久保先生

STRING: protein association networks

STRING: protein association networks

STRING: protein association networks

STRING: Protein association networks

STRING: Protein association networks

STRING: Protein association networks

STRING - Prediction of a functional association network for the yeast mitocho...

STRING - Prediction of a functional association network for the yeast mitocho...

STRING - Prediction of a functional association network for the yeast mitocho...

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Reviews to Peter Gariaev Book "Quantum Consciousness of the Linguistic-Wave ...

Visualization Approaches for Biomedical Omics Data: Putting It All Together

Visualization Approaches for Biomedical Omics Data: Putting It All Together

Visualization Approaches for Biomedical Omics Data: Putting It All Together

PhDc exam presentation

PhDc exam presentation

PhDc exam presentation

More from Rayna Harris

Course presentation that complements teaching via live coding. Inspired by the Software Carpentry Git Novice Lesson. Available online: http://swcarpentry.github.io/git-novice/. Citation: Ahmadia, A., Allen, J., Bachant, P., Banaszkiewicz, P., Barmby, P., Bekolay, T., … Wilson, G. (2015, May). Software Carpentry: Version Control with Git. Zenodo. http://doi.org/10.5281/zenodo.23544. Created for the Fall 2017 Center for Computational Biology and Bioinforamtics Short Course on Version Control for Bioinformatics with GitHub. http://ccbb.utexas.edu/ Slides available at: https://www.slideshare.net/raynamharris/version-control-with-github-for-bioinformatics

Version Control with GitHub for Bioinformatics

Version Control with GitHub for Bioinformatics

Version Control with GitHub for Bioinformatics

This was the very first talk I gave in front of a classroom based heavily on my research own personal research. It was 2013, and my audience was a group of students at the Marine Biological Lab in Woods Hole, MA and a group of students who Skyped in from Uruguay. The goal of this talk is to help students better understand when candidate gene approaches are preferred of whole genome approaches and vice versa. You can watch this talk here: http://videocenter.mbl.edu/videos/video/630/in/channel/21/

Time and Money: Techniques for Neural Gene Expression Profiling

Time and Money: Techniques for Neural Gene Expression Profiling

Time and Money: Techniques for Neural Gene Expression Profiling

Toward Single Neuron Gene Expression Analysis for Studying Behavior

Toward Single Neuron Gene Expression Analysis for Studying Behavior

Toward Single Neuron Gene Expression Analysis for Studying Behavior

This talk was given as a guest lecture in the undergraduate "Comparative Animal Physiology" course at the University of Texas at Austin. The talk has three parts: 1) a very brief personal introduciton about me and my thesis research, 2) an overview of some brain regions and genes that regulate social behaviors in birds, frogs, fish, reptiles, and mammals, 3) a discussion of a study looking at neuromolecular differences between closely related with with different mating systems.

Evolution of Social Brains

Evolution of Social Brains

Evolution of Social Brains

The poster was made during a 2.5 day NSF sponsored workshop on August 5th – 7th on the University of Washington, Seattle campus, which brought together 100 graduate students from diverse domain sciences and engineering with Data Scientists from industry and academia to discuss and collaborate on Big Data / Data Science challenges. In addition to keynote presentations from high profile speakers, the participants presented posters covering their own research and worked collaboratively to begin to solve some of the Grand Challenge problems facing Data Enabled Science & Engineering disciplines. This presentation was made by 10 graduate students, whose united them was high-dimensional biological data. For more info, see http://depts.washington.edu/dswkshp/

X team 2 - presentation

X team 2 - presentation

X team 2 - presentation

Neurobiology of Social Sensory Integration and Behavior

Neurobiology of Social Sensory Integration and Behavior

Neurobiology of Social Sensory Integration and Behavior

More from Rayna Harris (6)

Version Control with GitHub for Bioinformatics

Version Control with GitHub for Bioinformatics

Version Control with GitHub for Bioinformatics

Time and Money: Techniques for Neural Gene Expression Profiling

Time and Money: Techniques for Neural Gene Expression Profiling

Time and Money: Techniques for Neural Gene Expression Profiling

Toward Single Neuron Gene Expression Analysis for Studying Behavior

Toward Single Neuron Gene Expression Analysis for Studying Behavior

Toward Single Neuron Gene Expression Analysis for Studying Behavior

Evolution of Social Brains

Evolution of Social Brains

Evolution of Social Brains

X team 2 - presentation

X team 2 - presentation

X team 2 - presentation

Neurobiology of Social Sensory Integration and Behavior

Neurobiology of Social Sensory Integration and Behavior

Neurobiology of Social Sensory Integration and Behavior

Recently uploaded

Mustard, as a crop, is susceptible to a variety of pests that can affect its growth and yield. Here’s a rundown of some common pests that target mustard plants: Aphids: These small, sap-sucking insects can cause significant damage by feeding on the leaves and stems. Aphids also excrete a sticky substance known as honeydew, which can lead to the growth of sooty mold on the plants. Flea Beetles: These tiny beetles jump like fleas when disturbed and chew small holes in the leaves. They are particularly damaging in the early growth stages of the plant. Cabbage Loopers: The larvae of a type of moth, these caterpillars are known for their distinctive looping movement. They chew large holes in the leaves and can defoliate plants if present in large numbers. Diamondback Moth Larvae: Another caterpillar pest, these larvae chew small holes in the leaves and can cause extensive damage, especially when infestations are heavy. Whiteflies: These are tiny, winged insects that feed on plant sap and can quickly become a problem in greenhouse or close planting conditions. Like aphids, they also secrete honeydew. Cutworms: These are the larvae of certain types of moths and are known for cutting young plants at the stem base at ground level. They are most destructive during the night. Root Maggots: The larvae of root maggot flies, these pests attack the roots of mustard plants, causing wilting and potentially killing young plants. Harlequin Bugs: These are colorful stink bugs that suck the sap from mustard plant stems and leaves, causing the leaves to become stippled, wilt, and eventually die if the infestation is severe. Mustard Sawfly: The larvae of the mustard sawfly can cause considerable defoliation, as they feed voraciously on the leaves. Clubroot: Caused by a fungus-like organism, clubroot affects the roots, causing them to swell and distort. While technically a disease, it is often associated with pest management because controlling it involves similar preventative strategies. Control Measures: Managing pests in mustard involves a combination of cultural, biological, and chemical methods. Crop rotation, resistant varieties, timely sowing, maintaining plant health, and using natural predators like ladybugs and parasitic wasps can help keep pest populations under control. Chemical pesticides should be used as a last resort due to their potential impact on the environment and non-target species.

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

The computation of anti-derivatives is just an in-tellectual challenge, we know how to take deriv-atives, but … can we invert the process? We call this Computing the indefinite integral . In the last presentation we have seen a few indefinite integrals (we called them bricks), but they did not include the anti-derivative of many functions! We are going to try and do better !

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

CELL -Structural and Functional unit of life.pdf

CELL -Structural and Functional unit of life.pdf

CELL -Structural and Functional unit of life.pdf

Nistarini College, Purulia (W.B) India

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

RizalinePalanog2

Theoretical predictions and observational data indicate a class of sub-Neptune exoplanets may have water-rich interiors covered by hydrogen-dominated atmospheres. Provided suitable climate conditions, such planets could host surface liquid oceans. Motivated by recent JWST observations of K2-18 b, we self-consistently model the photochemistry and potential detectability of biogenic sulfur gases in the atmospheres of temperate sub-Neptune waterworlds for the first time. On Earth today, organic sulfur compounds produced by marine biota are rapidly destroyed by photochemical processes before they can accumulate to significant levels. Domagal-Goldman et al. suggest that detectable biogenic sulfur signatures could emerge in Archean-like atmospheres with higher biological production or low UV flux. In this study, we explore biogenic sulfur across a wide range of biological fluxes and stellar UV environments. Critically, the main photochemical sinks are absent on the nightside of tidally locked planets. To address this, we further perform experiments with a 3D general circulation model and a 2D photochemical model (VULCAN 2D) to simulate the global distribution of biogenic gases to investigate their terminator concentrations as seen via transmission spectroscopy. Our models indicate that biogenic sulfur gases can rise to potentially detectable levels on hydrogen-rich water worlds, but only for enhanced global biosulfur flux (20 times modern Earth’s flux). We find that it is challenging to identify DMS at 3.4 μm where it strongly overlaps with CH4, whereas it is more plausible to detect DMS and companion byproducts, ethylene (C2H4) and ethane (C2H6), in the mid-infrared between 9 and 13 μm. Unified Astronomy Thesaurus concepts: Exoplanet atmospheres (487); Exoplanet

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Site Acceptance Test .

Site Acceptance Test .

Site Acceptance Test .

Poonam Aher Patil

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Conjugation, transduction and transformation

Conjugation, transduction and transformation

Conjugation, transduction and transformation

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

Bacterial Identification and Classifications

Bacterial Identification and Classifications

Bacterial Identification and Classifications

Forensic Biology & Its biological significance.pdf

Forensic Biology & Its biological significance.pdf

Forensic Biology & Its biological significance.pdf

rohankumarsinghrore1

Vip CALL GIRL IN Lonavala 9748763073❤CALL GIRLS IN ESCORT SERVICE❤CALL GIRL IN We are Providing :- ● – Private independent collage Going girls . ● – independent Models . ● – House Wife’s . ● – Private Independent House Wife’s ● – Corporate M.N.C Working Profiles . ● – Call Center Girls . ● – Live Band Girls . ●- Foreigners & Many More . Service type: 1.In call 2.out call 3. full Lip to Lip kiss 4.69 5.b-job without Condom 6. Hard Core sex & Much More. 7 Body to Body Touch 8 Kissing 9 Sucking Boobs and More 10 Enjoy by Hand 11 Relax By Oral 12 Sex with Happy Ending • In Call and Out Call Service • 3* 5* 7* Hotels Service • 24 Hours Available • Indian, Russian, Punjabi, Kashmiri Escorts • Real Models, College Girls, House Wife, Also Available • Short Time and Full Time Service Available • Hygienic Full AC Neat and Clean Rooms Avail. In Hotel 24 hours • Daily Escorts Staff Available • Minimum to Maximum Range Available.c

Vip profile Call Girls In Lonavala 9748763073 For Genuine Sex Service At Just...

Introduction,importance and scope of horticulture.pptx

Introduction,importance and scope of horticulture.pptx

Introduction,importance and scope of horticulture.pptx

Bhagirath Gogikar

Just Call Vip call girls Srinagar Jammu Kashmir Escorts ☎️ 8617697112 Starting From 5K to 15K High Profile Escorts In Srinagar Jammu Kashmir ❤Personal Whatsapp Number Jammu Kashmir Call Girls 8617697112 💦✅. There are a number of Srinagar Jammu Kashmir Escorts willing to meet you at an affordable rate, which also possesses high moral standards and humanitarian tendencies. These girls can help satisfy the sexual desires of clients without fail; it is therefore essential that clients select an established service. Our services feature various packages at competitive rates: One shot: ₹2000/in-call, ₹5000/out-call Two shots with one girl: ₹3500/in-call, ₹6000/out-call Body to body massage with sex: ₹3000/in-call Full night for one person: ₹7000/in-call, ₹10000/out-call

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

GBSN - Microbiology (Unit 1)

GBSN - Microbiology (Unit 1)

GBSN - Microbiology (Unit 1)

module for grade 9 for distance learning

module for grade 9 for distance learning

module for grade 9 for distance learning

Choose Our GTB Nagar Call Girls Through Our WhatsApp Number: - 8250077686 Easy & Fast Booking Procedure of GTB Nagar Call Girls Call girls are ready and waiting to provide the quality essentialness that will help ease away life's tensions GTB Nagar Call Girls are regular companions of elite gentlemen. They can take them places they could only dream of visiting. These ladies take great care in maintaining themselves to look appealing; visiting salons and spas regularly keeps them looking their best; their portfolios can be found online via an escort agency website. Visit our website: https://www.prishagill.com/ Visit our website: https://www.dighacallgirls.com/ Visit our website: https://www.sheetalarora.com/ Visit our website: https://www.streetgirls69.in/

High Profile 🔝 8250077686 📞 Call Girls Service in GTB Nagar🍑

Clean In Place(CIP).pptx .

Clean In Place(CIP).pptx .

Clean In Place(CIP).pptx .

Poonam Aher Patil

Recently uploaded (20)

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pests of mustard_Identification_Management_Dr.UPR.pdf

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

Pulmonary drug delivery system M.pharm -2nd sem P'ceutics

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

COMPUTING ANTI-DERIVATIVES(Integration by SUBSTITUTION)

CELL -Structural and Functional unit of life.pdf

CELL -Structural and Functional unit of life.pdf

CELL -Structural and Functional unit of life.pdf

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

Chemical Tests; flame test, positive and negative ions test Edexcel Internati...

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

SCIENCE-4-QUARTER4-WEEK-4-PPT-1 (1).pptx

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds

Site Acceptance Test .

Site Acceptance Test .

Site Acceptance Test .

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Locating and isolating a gene, FISH, GISH, Chromosome walking and jumping, te...

Conjugation, transduction and transformation

Conjugation, transduction and transformation

Conjugation, transduction and transformation

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry

Bacterial Identification and Classifications

Bacterial Identification and Classifications

Bacterial Identification and Classifications

Forensic Biology & Its biological significance.pdf

Forensic Biology & Its biological significance.pdf

Forensic Biology & Its biological significance.pdf

Vip profile Call Girls In Lonavala 9748763073 For Genuine Sex Service At Just...

Introduction,importance and scope of horticulture.pptx

Introduction,importance and scope of horticulture.pptx

Introduction,importance and scope of horticulture.pptx

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.

GBSN - Microbiology (Unit 1)

GBSN - Microbiology (Unit 1)

GBSN - Microbiology (Unit 1)

module for grade 9 for distance learning

module for grade 9 for distance learning

module for grade 9 for distance learning

High Profile 🔝 8250077686 📞 Call Girls Service in GTB Nagar🍑

Clean In Place(CIP).pptx .

Clean In Place(CIP).pptx .

Clean In Place(CIP).pptx .

Hippocampal transcriptomic responses to technical and biological perturbations

1. Hippocampal transcriptomic responses to technical and biological perturbations Rayna M. Harris Neural Systems & Behavior Course July 24, 2017

2. Technological advances now allow single-cell transcriptomics Lane K et at. (2017) Measuring Signaling and RNA- Seq in the Same Cell Links Gene Expression to Dynamic Patterns of NF-κB Activation. Cell Syst

3. Challenge: Brain tissues consists of intricate neural layers & circuits Brain Tissue Single Neurons

4. Research Questions • How does the process of cellular dissociation alter gene expression? • Does the process of cellular dissociation alter our ability to identify biologically meaningful patterns of gene expression?

5. Approach Up-regulated genes and pathways Down-regulated genes and pathways Cembrowski et at. (2016) eLife

6. ‘Tissue punch’ sample collection

7. Reproducible Bioinformatics https://github.com/raynamharris/DissociationTest

8. Git for version control and GitHub for sharing data and analysis https://www.youtube.com/watch?v=taeAqimxXWo

9. The effect of cellular dissociation on hippocampal transcriptomes Harris RM et at. (2017) BioRxiv

10. The effect of a stressful experience on hippocampal transcriptomes Harris RM et at. (2017) BioRxiv

11. The effect of a cognitive training on hippocampal transcriptomes Harris RM et at. (2017) BioRxiv

12. Unique and shared responses to technical and biological perturbations Harris RM et at. (2017) BioRxiv

13. Meta analysis of reveals speciﬁc and robust patterns of expression Harris RM et at. (2017) BioRxiv

15. Thanks! • Hans Hofmann & all the Hofmann lab members • UT Genomic Sequencing & Analysis Facility • UT Center for Computational Biology & Bioinformatics • Misha Matz & Rachel Wright • Boris Zemelman, Laura Colgin • Andre Fenton, Maddy Kao, Ain Chung, Juan Marcos Alarcon at NYU & SUNY Downstate • The Neural Systems & Behavior Community • Software/Data Carpentry • NSF, NIH, Grass Foundation, Promega, JAX Lab 15