physiology exp for studying

1. 2/28/2021
1st year- Physiology Lab
1

2. 2/28/2021
1st year- Physiology Lab 2

3. 9. Effect of Exercise on
pulse rate.
Lab Teaching staff (Boys)
Mr. Muhammad Amir Mustufa
Dr. Muhammad Irfan

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Learning Objectives
2/28/2021
1st year- Physiology Lab
By the end of the practical students should be
able to:
 Identify Radial, Carotid, and brachial pulses in a subject.
 Record Radial pulse in resting state and after physical
exercise.
 Describe division of nervous system.
 Describe origin, types and functions of the autonomic
nervous system.
 List differences between sympathetic and parasympathetic
nervous system.

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Practical Outline
• Composition of Nervous system
• Differences between sympathetic and
parasympathetic nervous system.
• Regulatory effects of autonomic nervous
system.
• Procedure of radial pulse recording.
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6. Nervous system regulates body activities rapidly
through nerve impulses (action potentials).
Nervous system is divided into two main parts:
 A. Central Nervous System (CNS)
Brain and spinal cord.
 B. Peripheral Nervous System (PNS)
Composed of all nerves coming out or into the CNS.
These include spinal and cranial nerves. (Somatic &
autonomic nervous system)
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Introduction

7. NS
CNS
Brain
Spinal
Cord
PNS
Somatic NS
(sensory&
motor)
ANS
(sensory&
motor)
Symp.NS Paras.NS
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8. PNS Division
Peripheral
NS
Sensory & motor
neurons
Somatic NS – Voluntary
Input from sense organ
Output to skeletal muscles
Autonomic NS --
Involuntary
Input from internal receptor
Output to heart, smooth
muscles and glands
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9. ANS Division
Autonomic nervous
system
Involuntary
Input from internal receptor
Output to heart, smooth muscles &
glands
Sympathetic Motor
system
‘Fight or flight’ responses
Neurotransmitter : noradrenaline
‘Adrenergic System’
Parasympathetic Motor
System
Relaxing response
Neuroransmitter : acytylcholine
‘Cholinergic System’
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12. Comparison of the effect of sympathetic and
parasympathetic nervous system on
different systems & glands
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13. Recording radial & carotid pulse
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14. Procedure
The subject should be in a resting state
(physically and mentally) before recording
the pulse. Make the subject to lie down/sit
down comfortably at the examination
table/chair for some time.
Place fingertips (ring, middle and index
finger) over the radial artery in the
ventrolateral region near the subject's wrist
joint.
Feel and count the number of pulses for
30Sec. and note them down by multiplying
with 2 to convert it into the number of heart
beats per minute. 28/02/2021
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15.  Repeat the pulse counting twice and note down the
reading in pulses per min.
 Calculate the average of these three values and
note down in the table.
 Repeat the whole above procedure after proper
physical exercise. For exercise purposes, run and
take three to five rounds in the lab corridor or if
treadmill is available do exercise at least 2-3
minutes.
 Measure the radial pulse and record your
observation in the given table.
 Note the time of the radial pulse come to the
normal resting state after exercise.
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Procedure

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Observation
Observed values of pulse rate obtained before and after exercise
obs.
Before exercise
repeat 3 times
After exercise (one observation only)
Immediately 1 min 2 min 3 min 4 min 5 min
1
2
3
Average

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Sympathetic NS
Parasympathetic
NS

18. Identify the artery used in above picture for pulse
recording:
A. Brachail artery.
B. Carotid artery.
C. Radial artery.
D. Femoral artery.
E. Popliteal artery.
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19. Parasympathetic nervous system increases
the activity of:
A. Cardiovascular system.
B. Digestive system.
C. Respiratory system.
D. Sweat glands.
E. Piloerector muscles
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20.  Guyton Text book of medical Physiology 13th
edition.
 Hutchisons clinical methods 20th edition.
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References

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Thank You
2/28/2021
1st year- Physiology Lab