Parallel plate flow microscopy measures attachment or detachment of cells to substrate under blood vessel-like fluid flow conditions.

1. Cellular detachment assay Schematic for Parallel-plate Flow (PPF) Hard plastic chamber Plate with ECM absorbed Thin rubber gasket buffer buffer + cells

2. T lymphocytes adhered to fibronectin

3. Activated T lymphocytes adhered to fibronectin

4. Shear Stress 6 μ Q w h 2 µ = viscosity of the media (0.007) Q = the flow rate in cm 3 /s (dyne/cm 2 ) w = width of chamber h = height of chamber Venous shear stress: 1-6 dyne/cm 2 Arterial shear stress: 10-70 dyne/cm 2 (Malek, et al., JAMA, 1999)

5. Data: Activated T cell adhesion to FN

6. Data: CD45RA and RO T cell adhesion to monoclonal antibodies (dyne/cm 2 )