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Network biology
A crash course on STRING and
Cytoscape
Lars Juhl Jensen
STRING
functional associations
9.6 million genes
string-db.org
pathways
protein complexes
interaction screens
gene coexpression
genomic context
many databases
different formats
different identifiers
variable quality
not comparable
hard work
(Ph.D. students)
quality scores
affinity purification
von Mering et al., Nucleic Acids Research, 2005
score calibration
von Mering et al., Nucleic Acids Research, 2005
implicit weighting by quality
common scale
missing most of the data
>10 km
too much to read
text mining
comprehensive lexicon
cyclin dependent kinase 1
CDC2
spaces and hyphens
cyclin dependent kinase 1
cyclin-dependent kinase 1
prefixes and suffixes
CDC2
hCdc2
“black list”
SDS
co-mentioning
Cytoscape
visualization
analysis
large networks
not a database
STRING App
Acknowledgments
Damian Szklarczyk
John "Scooter" Morris
Helen Cook
Michael Kuhn
Stefan Wyder
Milan Simonovic
Alberto Santos
Nadezhda Doncheva
Alexander Roth
Peer Bork
Christian von Mering

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