seminario biologia molecular juan jose velasquez uribe

1. PRESENTED BY: JUAN JOSE VELASQUEZ URIBE
THIRD SEMESTER
2020-01

2. INTRODUCTION- antitumoral drugs
Platinum drugs
 low molecular weight alkylating drug
 cytotoxic effect: inhibition of DNA synthesis. intercalates into the double helix of
DNA and establishes intra and intercatenary bonds
 stops the cell cycle in the G2 phase and activates apoptosis
 Nephrotoxicity: directly induces necrosis and apoptosis of tubular kidney cells

3. DELIVERY SYSTEMS DRUGS
 biocompatible polymeric materials
 Lycium barbarum polysaccharide
 fruit of the goji berry (Solanaceae)
 LBP: extracellular signal-regulated kinase which in turn affects p53 expression
 regulating tumor apoptosis induce cell cycle arrest
of a variety of cancer cells at G0/G1, S, or G2/M phase

4. OBJECTIVES
they investigated The assembly and antitumor activity of
lycium barbarum polysaccharideplatinum-based conjugates

5. MATERIALES Y METODOS
 PANC-1 (human pancreatic cancer)
 MCF-7 (human breast cancer)
 293A (human normal kidney)
 A549 (human lung cancer cell line)
 las células se probaron midiendo la mitad
inhibidor máximo de concentración (IC50)

6. SINTESIS DEL CONJUGADO LBP-5-ASA
 Los conjugados LBP-5-ASA se sintetizaron a través de la base de Schiff reacción
entre LBP-CHO y 5-ASA
 el grupo aldehído de LBP oxidada reaccionó con el grupo amino del ácido 5-
aminosalicílico (5-ASA) para formar la base de Schiff.
 5-ASA se usó para coordinar complejos de Pt con el fin de construir nuevos
fármacos de Polisacáridos (conjugados LBP-5ASA-Pt),

7. CITOTOXICIDAD
 Las citotoxicidades de los conjugados LBP, LBP-5-ASA y LBP-5-ASA-Pt
 fueron expresados por la concentración de inhibición (IC50).
 Las células PANC-1, MCF-7, A549 y 293A se utilizaron para estudiar el potencial
terapéutico de LBP-5-ASA-Pt se conjuga como material terapéutico tumoral in
vitro.

8. ELECTROFORESIS
 se llevó a cabo para investigar la interacción de
los conjugados LBP, 5-ASA-Pt con ADN
 Finalmente, los resultados de la incubación. de
electroforesis en gel de agarosa
 ADN se incubó con conjugados LBP-5-ASA-Pt
durante 24 ha diferentes concentraciones.

9. CITOMETRIA DE FLUJO
 Se analizó el contenido de ADN de las
células para estudiar el ciclo celular.
 distribución de fases de células A549 en
respuesta a los conjugados LBP-5-ASA-Pt
 Fueron tratados con Rnase
 Se obtuvieron un total de 10,000 eventos
y el porcentaje de células en cada etapa
se determinó haciendo referencia a
células no tratadas

10. RESULTADOS
FT-IR analysis
groupuccessful conjugation
of 5-ASA to LBP

11. Anti-cancer activity of LBP-5-ASA-Pt conjugates
against A549 cells

12. Interaction of LBP-5-ASA-Pt conjugates with
DNA
DNA negative to
positive electrodes
the mobility of DNA is
slightly delayed
damage induced upon
binding of LBP-5-ASA-
Pt conjugates to DNA
interferes with normal
transcription and/or
DNA replication
lead to cell death
Lane 3: 3.6 μM;

13. Fluorescent image
LBP-5-ASA-Pt conjugates
for 48 h at the different
concentrations, nuclei
containing condensed
chromatin obviously
increased, suggesting that
apoptosis of A549 cells was
significantly induced

14. DISCUSSION Author mentioned was mentioned
J. Qiu The effects of LBP, LBP-5-
ASA and LBP-5-ASA-Pt
conjugates, 5-ASA,
oxaliplatin and ASA-Pt
complex on cell viability
were tested using the
MTT assay
K.J. Haxton Pt was bonded with LBP-
5- ASA including has -
COOH and -OH as the
leaving group of platinum
drugs
A.V. Nascimento and S.
O’Grady
Pt as a crosslinking agent,
binds to DNA to form Pt-
DNA adducts resulting in
irreversible lesions in the
double helix and ultimate
cell death

15. conclusions
 1. it was evident that the use of different concentrations of the conjugate gave
good results regarding the induction of apoptosis in certain types of cancer
 different variables must be found in order to explain why the conjugate had an
effect on certain cells such as the use of conjugates with platinum gave better
results

16. Bibliografía
 https://www.hindawi.com/journals/bmri/2019/4615745/
 https://www.sciencedirect.com/science/article/pii/S0308814618301973
 https://es.made-in-china.com/co_eyechem/product_Goji-Berry-Extract-Barbury-
Wolfberry-Fruit-Extract-with-Lycium-Barbarum-Polysaccharides_ryohierhg.html
 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3756417/