rapidfire

1. Delivery of Small and Large Molecules From A Hydrogel Via Light
Jessica L. Schlosser
UCLA Department of Bioengineering
Quantify:
Hydrogel Network Pyridine-2-thione
PEG Macromer Concentration
S S S S
+
I
S
SH
S
(can monitor at Enzymatic Activity
S-S- λ=345 nm)
S S S S
S S
Activity in presence of live cells
SH
Pyridine Disulfide Photodegradable Group Therapeutic peptide (GSH)/
Exchange and Release Activity
Lysozyme and BSA retain full enzymatic
• Glutathione contains a cysteine PEG 10K DA
APS/TEMED activity (substrate: p-nitrophenylacetate).
• Lysozyme was functionalized with
a free thiol hydrogel TGF-β1 induces GAG production in hMSCs
• BSA and TGF-β1 each contain an
depot after three days, visualized by toluidine blue
accessible cysteine, exchanged and verified with DMMB assay.
R= GSH, lysozyme: post-gelation exchange
pre-gelation R = BSA, TGF-β1: pre-gelation exchange
a hMSCs (- control) b hMSCs with TGF- 1 (+ control)
All gels exposed to light ([I]=10mW/cm2 λ =365 nm). The peptide/protein was equilibrated in solution
for 24 hours prior to quantifying release.
light 365 nm light
100 m 100 m
c hMSCs with released TGF- 1 d 10
normalized [GAG]/cell
0
5
0
released TGF- 1
10 ng/mL TGF- 1
normalized [GAG]
no TGF- 1
(- control)
5.2 ng/mL
- control + control
(+ control)
100 m