3D-Screen Technology overview

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3D-Screen Technology overview

  1. 1. A new way to Drug Discovery
  2. 2. The 3D-Screen technology A new way to Drug Discovery > Biological products exert their function through a specific tri-dimensional conformation that controls their interactions with cellular partners Known Ligand TARGET TARGET PROTEIN Biological PROTEIN Response Dramatic or subtle conformational changes ®2 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  3. 3. The 3D-Screen technology Basic Principle ON OFF Native conformation of the target is recognize by the 3D- Sensor TARGET PROTEIN Identification in a single assay of: COMPOUND TARGET LIBRARY -Agonist PROTEIN -Antagonist - Allosteric Modulator (Inhibitor & Activator) - Cellular cofactor TARGET modulator PROTEIN Screening for CONFORMATION changes ®3 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  4. 4. 3D-SCREENTM Technology Molecular principle & Opportunities offered COMPOUND AD LIBRARY Peptide-sensor CELLULAR COFACTOR HCV CELLULAR COFACTOR TARGET CELLULAR COFACTOR HUMAN CELL DBD 5’ Luciferase 3’ UAS DISCOVERY OF THE BROAD RANGE OF DRUGS - Agonists (DAA)AD: Activating Domain - Antagonists (DAA)DBD: DNA Binding DomainUAS: upstream Activating Sequence - Allosteric Modulators (DAA) - Disruption of protein interactions (HTA) - Modulators of cellular cofactors (HTA) ®4 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  5. 5. 3D-SCREENTM Technology Molecular principle & Opportunities offered COMPOUND LIBRARY CELLULAR AD COFACTOR Peptide-sensor HCV CELLULAR TARGET COFACTOR CELLULAR COFACTOR HUMAN CELL DBD 5’ Luciferase 3’ UAS X DISCOVERY OF THE BROAD RANGE OF DRUGS - Agonists (DAA)AD: Activating Domain - Antagonists (DAA)DBD: DNA Binding DomainUAS: upstream Activating Sequence - Allosteric Modulators (DAA) - Disruption of protein interactions (HTA) - Modulators of cellular cofactors (HTA) ®5 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  6. 6. 3D-Sensor: The Heart of 3D-Screen technology A powerful cell-based assay to discover allosteric & cellular cofactor modulators of HCV NS5B polymerase IN ONE SINGLE ASSAY ®6 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  7. 7. 3D-Sensor: The Heart of 3D-Screen technology A powerful cell-based assay to discover allosteric & cellular cofactor modulators of HCV NS3 protein IN ONE SINGLE ASSAY ORTHOSTERIC MODULATORS ORIGINAL MODULATORS Peptidomimetic anti-protease inhibitors Small molecule (< 500 D) Linear Macrocyclic CVVS-060215 (VIVALIS) (e.g VX950) (e.g BILN2061) (EC50 =13 nM) + + + VIVALIS SMALL MOLECULE UNDER LEAD OPTIMIZATION WHICH IS NOT A PEPTIDOMIMETIC OR A MACROCYCLIC MOLECULE UNLIKE MOST COMPETITOR MOLECULES ®7 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  8. 8. 3D-Sensor: The Heart of 3D-Screen technology A powerful cell-based assay to discover any modulators’ class of Estrogen Receptor alpha ACCORDING SCREENING SPECIFICATIONS CONFORMATION SENSITIVE PEPTIDES CAN BE IDENTIFIED THAT ARE SENSITIVE TO AGONISTS, ANTAGONISTS OR BOTH NO E2 4OHT 9cRA DEX LIGAND agonist antagonist RA ligand GR ligand 1 10nM 50µM 0.5µM µM Selected peptide sensitives to all types of ligands Selected peptides sensitive to antagonist ligands only Selected peptide sensitives to agonist ligands only SELECTION OF THE 3D-SENSOR CANDIDATE ACCORDING TO THE INTENTED SCREENING OBJECTIVES ®8 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  9. 9. 3D-Sensor: The Heart of 3D-Screen technology A powerful cell-based assay with a VERY HIGH SENSITIVITY 3D-Sensor sensitive to conformational changes of Retinoic Acid Receptor (RAR) induced by the binding of all-trans-RA only or 9-cis-RA & all-trans-RA in Yeast cells Inhibition by 9-cis-RA & all-trans-RA Inhibition by all-trans-RA onlyRA : RETINOIC ACID 9-cis-RA All-rans-RA Selective identification of 3D-Sensor sensing subtle differences in conformational changes induced by the structurally close at-RA and 9cis-RA ligands ®9 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  10. 10. The 3D-SCREENTM Technology An innovative platform for new classes of drugs 1. A unique proprietary human cell-based, target-oriented, HTS-compatible assay to screen for small molecules that specifically alter the 3D structure of a target protein in their natural cellular environment. 2. A powerful platform to discover orthosteric, allosteric & cellular cofactor modulators IN ONE SINGLE ASSAY, including molecules that cannot be identifed using tradional cell-based assays 3. Validated with well established therapeutic targets (Hepatitis C, Nuclear Receptors) 4. Applicable to any validated therapeutic target (viral proteins, nuclear receptors, kinases, …) A novel drug discovery platform that has the potential to produce clinically differentiated products across multiple therapeutic areas ®10 March 26, 2012© 2000-2011 Vivalis — All rights reserved— CONFIDENTIAL
  11. 11. ® Merci Thank Youありがとうございました

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