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ANALYSIS OF
VARIANCE
(ANOVA) or F-TEST
Harold P. Mediodia
Antipolo City
Dec 2008
• used in determining the significant
differences among the means of more
than two groups
• variation both between and within
each of the group is analyzed to
establish the F value
One-way Analysis of
Variance
(One-Way ANOVA)
Example:
A study was conducted to determine
the effect of different stocking
densities on the gel strength of
agar gel produced by Gracilariopsis
bailinae. They used three different
stocking densities: 500, 1000, 2000
g/m². They extracted agar gel and
measured the gel strength in g/cm².
The One-Way ANOVA is useful in
this case for the following
reasons:
1. There were more than two means as
independent variable.
means of the gel strength of three
different stocking densities:
500 g/cm², 1000 g/cm² and 2000 g/cm²
The One-Way ANOVA is useful in
this case for the following
reasons:
2. There was only one independent
variable involved
stocking density of
Gracilariopsis bailinae
Two-Way Analysis
of Variance
(Two-Way ANOVA)
determine the main and
simultaneous effects of two
independent factors on one
or more dependent variables
Example:
A group studied the quality of mangoes
produced at different physiological
status (without flush, 25%-50% flush and
75%-90% flush) at different ages (105
DAFI, 110 DAFI and 120 DAFI). They
determined the quality of mangoes in
terms of weight of the mangoes produced.
DAFI- Days After Flower Introduction
The Two-Way ANOVA is
useful for the following
reasons:
1. There were more than two means
as independent variables.
means of weights: three
physiologic status for the first
independent variable and three
ages for the second independent
variable (nine means)
The Two-Way ANOVA is
useful for the following
reasons:
2. There were two independent
variable.
physiologic status and ages,
whose effect on the dependent
variables of the study were
simultaneously determined
Example:
Taxonomic Classiffication and Determine of
the Iodine Content of Seaweeds Collected
along the Coast of Brgy. Sabang, Sibunag,
Guimaras.
Objectives:
1. To collect, classify and identify seaweeds found
along the coast of Brgy. Sabang, Sibunag,
Guimaras.
2. To measure the iodine connent of selected
seaweeds collected along the coast of Brgy.
Sabang, Sibunag, Guimaras.
3. To compare the iodine content of seaweeds
collected along the coast of Brgy. Sabang,
Sibunag, Guimaras.
Table 1. Iodine Content (percent) of Seaweeds
Collected in the Coastal Area of Barangay Sabang,
Sibunag, Guimaras.
Gracilaria edulis 1.102 0.876 1.124
Gracilaria salicornia 0.737 0.859 1.056
Gracilaria acerosa 0.769 0.736 0.920
Gracilaria eucheumoides 1.149 0.790 0.721
ONE-WAY ANALYSIS OF
VARIANCE
1.H0: There is no significant difference
in the iodine content of seaweeds
collected from the coastal areas of
Barangay Sabang, Sibunag, Guimaras.
H1: There is a significant difference in
the iodine content of seaweeds
collected from the coastal areas of
Barangay Sabang, Sibunag, Guimaras.
2. Level of Significance: α=0.05
3. Degrees of Freedom
d.f for numerator = k-1
d.f for denominator = N-k
k= numbers of groups
N= total number of samples in all groups
d.f for numerator = 4 – 1 = 3
d.f for denominator = 12 – 4 = 8
4. Critical Region:
F0.05= 4.07
4.07
If the computed F value ≥ 4.07, the
null hypothesis is rejected.
5. Test Statistics:
One-way Analysis of
Variance (ANOVA)
6. Compute and fill-up the ANOVA Table
Source of Sum of Degrees of Mean F
Variation Squares Freedom Square Radio
(Variance)
Between 0.80250 3 0.026750 0.998283
Groups
Within 0.214364 8 0.026796
Groups
Total 0.294614 11
7. Statistical Decision
Since the computed F-value (0.998283) is less
than the critical region (4.07), there is no
sufficient evidence to reject the null hypothesis.
4.07
0.998283
8. Conclusion
There is no significant difference in the
iodine content of seaweeds collected from
the coastal areas of Barangay Sabang,
Sibunag, Guimaras.
Interpretation
The iodine content is the same or equal
for the four Gracilaria species.
Computing for F ...
G. edulis G. salicornia G. Acerosa G.
eucheumoides
N1 = 3 N2 = 3 N3 = 3 N4 = 3
X1 X1² X2 X2² X3 X3² X4 X4²
1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201
0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100
1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841
ΣX1 =
3.102
ΣX1²=
3.245156
ΣX2=
2.652
ΣX2²=
2.396186
ΣX3=
2.425
ΣX3²=
1.979457
ΣX4=
2.660
ΣX4²=
2.464142
N= n1 + n2 +n3 + n4 = 12
ΣXTOT= 10.839
ΣX²TOT= 10.084941
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between SSBET d.f BET MSBET MSBET
Groups
Within SSW d.fW MSW
Groups
Total SST N-1
MDW
SST= ΣX²TOT
(ΣXTOT)²
N
a.
= 10.084941 –
= 10.084941 – 9.790327
= 0.294614
(10.839)²
12
N = 12
ΣXTOT=
10.0839
ΣX²TOT=
10.084941
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between SSBET d.f BET MSBET MSBET
Groups
Within SSW d.fW MSW
Groups
Total 0.294614 N-1
SSBET= Σ
(ΣX1)²
N1[ ](ΣXTOT)²
N
(3.102)²
3
(2.652)²
3
(2.425)²
3
(10.839)²
12
= + + -(2.660)²
3
+
= 3.207468 + 2.344368 + 1.960208 + 2.358533 – 9.790327
= 0.080250
G. edulis G. salicornia G. Acerosa G.
eucheumoides
N1 = 3 N2 = 3 N3 = 3 N4 = 3
X1 X1² X2 X2² X3 X3² X4 X4²
1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201
0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100
1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841
ΣX1 =
3.102
ΣX1²=
3.245156
ΣX2=
2.652
ΣX2²=
2.396186
ΣX3=
2.425
ΣX3²=
1.979457
ΣX4=
2.660
ΣX4²=
2.464142
N= 12
EXTOT= 10.839
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between 0.080250 d.f BET MSBET MSBET
Groups
Within SSW d.fW MSW
Groups
Total 0.294614 N-1
MSW
c. SSW= Σ SS1
= SS1 + SS2 + ... + SSk
SS1= ΣX1² -
(ΣX1)²
N1
SS1= 3.245156 - = 0.037688
SS2= 2.396186 - = 0.051818
SS3= 1.979457 - = 0.019249
SS4= 2.464142 - = 0.105609
(3.102)²
3
(2.652)²
3
(2.425)²
3
(2.660)²
3
SSW = SS1 + SS2 + SS3 + SS4
= 0.037688 + 0.051818 + 0.019249 + 0.105609
= 0.214364
G. edulis G. salicornia G. Acerosa G.
eucheumoides
N1 = 3 N2 = 3 N3 = 3 N4 = 3
X1 X1² X2 X2² X3 X3² X4 X4²
1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201
0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100
1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841
ΣX1 =
3.102
ΣX1²=
3.245156
ΣX2=
2.652
ΣX2²=
2.396186
ΣX3=
2.425
ΣX3²=
1.979457
ΣX4=
2.660
ΣX4²=
2.464142
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between 0.080250 d.f BET MSBET MSBET
Groups
Within 0.214364 d.fW MSW
Groups
Total 0.294614 N-1
MSW
d. d.f.BET, d.f.W, and d.f.TOT
d.f.BET= k-1
d.f.W= N-k
d.f.TOT= d.f.BET + d.f.W= N - 1
= 4 – 1 = 3
= 12- 4 = 8
= 12 – 1 = 11
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between 0.080250 3 MSBET MSBET
Groups
Within 0.214364 8 MSW
Groups
Total 0.294614 11
MSW
e. Mean of Squares (MSBET and MSW)
SSBET
d.f.BET
SSw
d.f.W
MSBET= MSW=
= 0.080250
3
= 0.026750
= 0.214364
8
= 0.026796
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between 0.080250 3 0.26750 MSBET
Groups
Within 0.214364 8 0.026796
Groups
Total 0.294614 11
MSW
f. F-value
F =
MSBET
MSW
= 0.026750
0.026796
= 0.998283
The ANOVA Table
Source of Sum of Degrees of Mean F Ratio
Variation Squares Freedom Square
(Variance)
Between 0.080250 3 0.26750 0.998283
Groups
Within 0.214364 8 0.026796
Groups
Total 0.294614 11
6. Compute and fill-up the ANOVA Table
References:
Amparado, K.M., Pestaño, R., Suelo, J.K.. 2006.
Taxonomic Classification and Determination of the
Iodine Content of Seaweeds Collected along the Coast of
Brgy. Sabang, Sibunag, Guimaras. Unpublished Research
Paper. Philippines Science High School Western Visayas.
Brase, C.H., Brase, C.P,. 1995. Understandable
Statistics Concepts and Methods, D.c., Health and
Company. Lexington, Massachussettes.
Cadomigara, M., 2002. Fundamentals of Research, Methods
and Models Mindset Publishing, Inc. Iloilo City
Milton J.S., McTeer, p.m and Corbet, J.J., 1997.
Introduction to Statistics McGraw-Hill Companies, Inc.
United States of America

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Presentation1

  • 1. ANALYSIS OF VARIANCE (ANOVA) or F-TEST Harold P. Mediodia Antipolo City Dec 2008
  • 2. • used in determining the significant differences among the means of more than two groups • variation both between and within each of the group is analyzed to establish the F value One-way Analysis of Variance (One-Way ANOVA)
  • 3. Example: A study was conducted to determine the effect of different stocking densities on the gel strength of agar gel produced by Gracilariopsis bailinae. They used three different stocking densities: 500, 1000, 2000 g/m². They extracted agar gel and measured the gel strength in g/cm².
  • 4. The One-Way ANOVA is useful in this case for the following reasons: 1. There were more than two means as independent variable. means of the gel strength of three different stocking densities: 500 g/cm², 1000 g/cm² and 2000 g/cm²
  • 5. The One-Way ANOVA is useful in this case for the following reasons: 2. There was only one independent variable involved stocking density of Gracilariopsis bailinae
  • 6. Two-Way Analysis of Variance (Two-Way ANOVA) determine the main and simultaneous effects of two independent factors on one or more dependent variables
  • 7. Example: A group studied the quality of mangoes produced at different physiological status (without flush, 25%-50% flush and 75%-90% flush) at different ages (105 DAFI, 110 DAFI and 120 DAFI). They determined the quality of mangoes in terms of weight of the mangoes produced. DAFI- Days After Flower Introduction
  • 8. The Two-Way ANOVA is useful for the following reasons: 1. There were more than two means as independent variables. means of weights: three physiologic status for the first independent variable and three ages for the second independent variable (nine means)
  • 9. The Two-Way ANOVA is useful for the following reasons: 2. There were two independent variable. physiologic status and ages, whose effect on the dependent variables of the study were simultaneously determined
  • 10. Example: Taxonomic Classiffication and Determine of the Iodine Content of Seaweeds Collected along the Coast of Brgy. Sabang, Sibunag, Guimaras. Objectives: 1. To collect, classify and identify seaweeds found along the coast of Brgy. Sabang, Sibunag, Guimaras. 2. To measure the iodine connent of selected seaweeds collected along the coast of Brgy. Sabang, Sibunag, Guimaras. 3. To compare the iodine content of seaweeds collected along the coast of Brgy. Sabang, Sibunag, Guimaras.
  • 11. Table 1. Iodine Content (percent) of Seaweeds Collected in the Coastal Area of Barangay Sabang, Sibunag, Guimaras. Gracilaria edulis 1.102 0.876 1.124 Gracilaria salicornia 0.737 0.859 1.056 Gracilaria acerosa 0.769 0.736 0.920 Gracilaria eucheumoides 1.149 0.790 0.721
  • 12. ONE-WAY ANALYSIS OF VARIANCE 1.H0: There is no significant difference in the iodine content of seaweeds collected from the coastal areas of Barangay Sabang, Sibunag, Guimaras. H1: There is a significant difference in the iodine content of seaweeds collected from the coastal areas of Barangay Sabang, Sibunag, Guimaras. 2. Level of Significance: α=0.05
  • 13. 3. Degrees of Freedom d.f for numerator = k-1 d.f for denominator = N-k k= numbers of groups N= total number of samples in all groups d.f for numerator = 4 – 1 = 3 d.f for denominator = 12 – 4 = 8
  • 14. 4. Critical Region: F0.05= 4.07 4.07 If the computed F value ≥ 4.07, the null hypothesis is rejected.
  • 15. 5. Test Statistics: One-way Analysis of Variance (ANOVA)
  • 16. 6. Compute and fill-up the ANOVA Table Source of Sum of Degrees of Mean F Variation Squares Freedom Square Radio (Variance) Between 0.80250 3 0.026750 0.998283 Groups Within 0.214364 8 0.026796 Groups Total 0.294614 11
  • 17. 7. Statistical Decision Since the computed F-value (0.998283) is less than the critical region (4.07), there is no sufficient evidence to reject the null hypothesis. 4.07 0.998283
  • 18. 8. Conclusion There is no significant difference in the iodine content of seaweeds collected from the coastal areas of Barangay Sabang, Sibunag, Guimaras. Interpretation The iodine content is the same or equal for the four Gracilaria species.
  • 19. Computing for F ... G. edulis G. salicornia G. Acerosa G. eucheumoides N1 = 3 N2 = 3 N3 = 3 N4 = 3 X1 X1² X2 X2² X3 X3² X4 X4² 1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201 0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100 1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841 ΣX1 = 3.102 ΣX1²= 3.245156 ΣX2= 2.652 ΣX2²= 2.396186 ΣX3= 2.425 ΣX3²= 1.979457 ΣX4= 2.660 ΣX4²= 2.464142 N= n1 + n2 +n3 + n4 = 12 ΣXTOT= 10.839 ΣX²TOT= 10.084941
  • 20. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between SSBET d.f BET MSBET MSBET Groups Within SSW d.fW MSW Groups Total SST N-1 MDW
  • 21. SST= ΣX²TOT (ΣXTOT)² N a. = 10.084941 – = 10.084941 – 9.790327 = 0.294614 (10.839)² 12 N = 12 ΣXTOT= 10.0839 ΣX²TOT= 10.084941
  • 22. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between SSBET d.f BET MSBET MSBET Groups Within SSW d.fW MSW Groups Total 0.294614 N-1
  • 23. SSBET= Σ (ΣX1)² N1[ ](ΣXTOT)² N (3.102)² 3 (2.652)² 3 (2.425)² 3 (10.839)² 12 = + + -(2.660)² 3 + = 3.207468 + 2.344368 + 1.960208 + 2.358533 – 9.790327 = 0.080250
  • 24. G. edulis G. salicornia G. Acerosa G. eucheumoides N1 = 3 N2 = 3 N3 = 3 N4 = 3 X1 X1² X2 X2² X3 X3² X4 X4² 1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201 0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100 1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841 ΣX1 = 3.102 ΣX1²= 3.245156 ΣX2= 2.652 ΣX2²= 2.396186 ΣX3= 2.425 ΣX3²= 1.979457 ΣX4= 2.660 ΣX4²= 2.464142 N= 12 EXTOT= 10.839
  • 25. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between 0.080250 d.f BET MSBET MSBET Groups Within SSW d.fW MSW Groups Total 0.294614 N-1 MSW
  • 26. c. SSW= Σ SS1 = SS1 + SS2 + ... + SSk SS1= ΣX1² - (ΣX1)² N1
  • 27. SS1= 3.245156 - = 0.037688 SS2= 2.396186 - = 0.051818 SS3= 1.979457 - = 0.019249 SS4= 2.464142 - = 0.105609 (3.102)² 3 (2.652)² 3 (2.425)² 3 (2.660)² 3 SSW = SS1 + SS2 + SS3 + SS4 = 0.037688 + 0.051818 + 0.019249 + 0.105609 = 0.214364
  • 28. G. edulis G. salicornia G. Acerosa G. eucheumoides N1 = 3 N2 = 3 N3 = 3 N4 = 3 X1 X1² X2 X2² X3 X3² X4 X4² 1.102,, 1.214404 0.737 0.543169 0.769 0.591361 1.149 1.320201 0.876 0.767376 0.859 0.737881 0.736 0.541696 0.790 0.624100 1.124 1.263376 1.056 1.115136 0.920 0.846400 0.721 0.519841 ΣX1 = 3.102 ΣX1²= 3.245156 ΣX2= 2.652 ΣX2²= 2.396186 ΣX3= 2.425 ΣX3²= 1.979457 ΣX4= 2.660 ΣX4²= 2.464142
  • 29. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between 0.080250 d.f BET MSBET MSBET Groups Within 0.214364 d.fW MSW Groups Total 0.294614 N-1 MSW
  • 30. d. d.f.BET, d.f.W, and d.f.TOT d.f.BET= k-1 d.f.W= N-k d.f.TOT= d.f.BET + d.f.W= N - 1 = 4 – 1 = 3 = 12- 4 = 8 = 12 – 1 = 11
  • 31. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between 0.080250 3 MSBET MSBET Groups Within 0.214364 8 MSW Groups Total 0.294614 11 MSW
  • 32. e. Mean of Squares (MSBET and MSW) SSBET d.f.BET SSw d.f.W MSBET= MSW= = 0.080250 3 = 0.026750 = 0.214364 8 = 0.026796
  • 33. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between 0.080250 3 0.26750 MSBET Groups Within 0.214364 8 0.026796 Groups Total 0.294614 11 MSW
  • 34. f. F-value F = MSBET MSW = 0.026750 0.026796 = 0.998283
  • 35. The ANOVA Table Source of Sum of Degrees of Mean F Ratio Variation Squares Freedom Square (Variance) Between 0.080250 3 0.26750 0.998283 Groups Within 0.214364 8 0.026796 Groups Total 0.294614 11 6. Compute and fill-up the ANOVA Table
  • 36. References: Amparado, K.M., Pestaño, R., Suelo, J.K.. 2006. Taxonomic Classification and Determination of the Iodine Content of Seaweeds Collected along the Coast of Brgy. Sabang, Sibunag, Guimaras. Unpublished Research Paper. Philippines Science High School Western Visayas. Brase, C.H., Brase, C.P,. 1995. Understandable Statistics Concepts and Methods, D.c., Health and Company. Lexington, Massachussettes. Cadomigara, M., 2002. Fundamentals of Research, Methods and Models Mindset Publishing, Inc. Iloilo City Milton J.S., McTeer, p.m and Corbet, J.J., 1997. Introduction to Statistics McGraw-Hill Companies, Inc. United States of America