How many grams of glucose c6h1206 will be needed to prepare 250mlof 5% glucose solution in water? How many grams of glucose c6h1206 will be needed to prepare 250mlof 5% glucose solution in water? Solution 5% glucose solution = 5 gm glucose in 95 ml water Hence amount of glucose needed to prepare = 5 x 2.5 = 12.5 gm Hence 12.5 gm of glucose is needed to prepare 250ml of 5% glucose solution in water .