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       Code No: RAR05322301
                                                                         RA
          III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
                  ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY
                                           (Bio-Technology)
       Time: 3 hours                                                                Max Marks: 80
                                    Answer any FIVE Questions
                                  All Questions carry equal marks



         1. Explain the following terms associated with Fluorescent Microscope.

             (a) Barrier lter
             (b) Heat lter

             (c) Fluorochromes
             (d) Exciter lter.                                                             [4+4+4+4]

         2. What is the di erence between a TEM and a SEM?                                       [16]

         3. How is blo od sugar measured?                                                        [16]

         4. What are the factors a ecting electrophoretic mobility of any molecule? [16]

         5. Explain the basic principle behind all forms of Chromatography [16]

         6. How is PCR used to sequence DNA?                                                     [16]

         7. Write short notes on

             (a) Rocket Immunoelectroforesis
             (b) Radial Immunoassays.
                                                                                                [8+8]

         8. Explain

             (a) Substrate uptake in absence of product formation.
             (b) Substrate uptake with product formation.                                       [8+8]




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22301 Analytical Techniques In Bio Technology Set1

  • 1. www.studentyogi.com www.studentyogi.com Code No: RAR05322301 RA III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Explain the following terms associated with Fluorescent Microscope. (a) Barrier lter (b) Heat lter (c) Fluorochromes (d) Exciter lter. [4+4+4+4] 2. What is the di erence between a TEM and a SEM? [16] 3. How is blo od sugar measured? [16] 4. What are the factors a ecting electrophoretic mobility of any molecule? [16] 5. Explain the basic principle behind all forms of Chromatography [16] 6. How is PCR used to sequence DNA? [16] 7. Write short notes on (a) Rocket Immunoelectroforesis (b) Radial Immunoassays. [8+8] 8. Explain (a) Substrate uptake in absence of product formation. (b) Substrate uptake with product formation. [8+8] 1 of 1 www.studentyogi.com www.studentyogi.com