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NEW TECHNOLOGIES REPORT - #06
Date:5th November , 2022
ENZYME ASSISTED
EXTRACTION
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The initial stage in separating the desired natural products from the base materials is
extraction.
• general method of extraction involves
• solvent extraction
• distillation method
• pressing
• sublimation
INTRODUCTION
GENERAL STAGES OF EXTRACTION
• The solvent penetrates into the solid matrix;
• The solute dissolves in the solvents;
• The solute is diffused out of the solid matrix;
• The extracted solutes are collected

ENZYME-ASSISTED EXTRACTION
• Enzyme-assisted extraction represents a potential bioprocess for the production/extraction
of phenolic compounds with high stability and high antioxidant activity for pharmaceutical
and food applications.
• Advanced extraction technologies are needed to meet the emerging requirements. The
most recent method for removing bio ingredients from plant materials uses enzyme assisted
extraction.
• Enzymes are categorised according to the compounds they act upon. Some of the most
common types include
1. Pro-teases (Which Break Down Proteins),
2. Cellulases (Which Break Down Cellulose),
3. Lipases (Which Split Fats Into Glycerol And Fatty Acids),
4. Amylases (which break down starch into simple sugars).

Extraction of Z-lingustilide
THE BASIC PRINCIPLE OF ENZYME
ASSISTED EXTRACTION

ENZYMATIC EXTRACTION OF
POLYPHENOLS FROM GREEN
YERBA MATE
Ana Carolina Winkler Heemann etal.,2019
1. Aim of this work was to evaluate the enzyme-assisted extraction of
polyphenols from green yerba mate employing response surface
methodology (RSM), in order to determine the best extraction
conditions.
2. The use of carbohydrases increased the extraction of polyphenols from
about 38.67% to 52.08%.
3. The enzyme-assisted extraction of polyphenols from green yerba mate
was considered a good alternative to conventional solvent extraction,
since enzymes are renewable matter that further contribute to
sustainable practices.

ENZYMATIC EXTRACTION OF
POLYSACCHARIDE
1. Polysaccharides are the most widely studied
group of metabolitesapart from polyphenols.
2. Blanco-Pascual et al. extracted carrageenan
from Mastocarpus stellatus using Alcalase®
(a type of protease). The extracted
polysaccharides showed excellent gelling
properties. The extraction process led to a
simultaneous extraction of valuable
phytochemicals such as polyphenols, thereby
adding value to the extract

ENZYMATIC EXTRACTION OF OILS
Conventional techniques such as hydraulic pressing, expeller
pressing and solvent extraction are used in oil extraction from seeds
Boulila et al. extracted essential oils and phenolics from bay
leaves (Laurus nobilis L.). The enzyme mixture of cellulase, hemicellulase, xylanase proved to be
useful to improve the extraction efficiency of biomolecules from plant matrix by degrading
plant cell wall.
When the substrate was pretreated with cellulase, hemicellulase, xylanase separately and their
ternary mixture, extraction yield of essential
oils increased by 243, 227, 240.54 and 248%, respectively. Further
analysis revealed that enzyme pretreated substrates showed enhancement in antioxidant
activity due to essential oils.

ENZYMATIC EXTRACTION OF COLOURS
Commercially available enzymes such as cellulase,
amylase and pectinase have been explored in the past, to solubilise the cell wall, leading to
the extraction of pigments and flavours
Enzyme-Assisted Extraction of Carotenoid-Rich Extract from Red Capsicum (Capsicum
annuum)
Observation: Three carbohydrases enzymes viz. viscozyme L, pectinase and cellulase were
tested for their liquefaction effects and ability to recover higher carotenoids in aqueous extract.
Incremental increase in dosage of enzymes significantly (p < 0.05) improved the extract yield,
total carotenoids, phenolics, ascorbic acid content and antioxidant activity. Viscozyme and
pectinase caused significantly higher recovery of carotenoids and other bioactives than
cellulase.

ADVANTAGES
• These green extraction methods not only reduce the requirement of hazardous solvents
but also require short extraction time.
• faster extraction rate
• simpler recovery with reduced solvent
• Additionally, as this extraction is carried out under controlled temperature condition, it is
very useful for extraction of thermo-sensitive molecules such as flavours, pigments, oil,
etc
Critical Operational Conditions
1. Temperature of reaction,
2. Time of extraction,
3. pH of system,
4. Enzyme concentration,and
5. Particle size of substrate

• Nadar, S. S., Rao, P., & Rathod, V. K. (2018). Enzyme assisted extraction of biomolecules as an
approachto novel extraction technology:A review. Food Research International,108, 309–
330. doi:10.1016/j.foodres.2018.03.006
• https://www.researchgate.net/publication/258212363_Enzyme_-
Assisted_extraction_of_Bioingredients
• https://cmjournal.biomedcentral.com/articles/10.1186/s13020-018-0177-x
REFERENCES

THANK YOU
M1. 20%

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