Which of the following factors does not affect membrane permeability? Temperature The saturation of hydrocarbon tails in membrane phospholipids The amount of cholesterol in the membrane The polarity of membrane phospholipids Solution The correct answer is option (4) the polarity of membrane phospholipids, because usually the phospholipids have hydrophobic and hydrophilic regions, so the polarity of phospholipids do not change the permeability. The main factors affect the permeability of membrane are:.