1. SYNOPSIS
“APPLICATION OF BONE MARROW SUPPRESSIVE AGENTS:
EFFECTS ON HEMATOPOIETIC SYSTEM AND FUNCTIONAL
MANIFESTATION BY SPLENOCYTES”.
BY: - ANJAN GHOSH
REG. No. 3166 OF 2007-2008
M.Sc. MEDICAL BIOTECHNOLOGY (II)
CALCUTTA SCHOOL OF TROPICAL MEDICINE.
GUIDE: - Dr. SUJATA LAW
DEPARTMENT OF BIOCHEMISTRY & MEDICAL
BIOTECHNOLOGY
CALCUTTA SCHOOL OF TROPICAL MEDICINE
The present project deals with application of BUSALFAN as
haematopoiesis suppressive agent in inbred Swiss albino mouse. Functional
manifestation was carried out by observing the Peripheral Blood Haemogram.
Busalfan is a polyfunctional-alkylating agent, which can induce
haematopoietic failure that can be manifested by observing the peripheral blood
haemogram. Furthermore, by performing ‘single cell isolation technique’, the
splenocytes were isolated which contain lymphocytes, macrophages etc.
NitroBlueTetrazolium Assay was performed on these isolated splenocytes.
NitroBlueTetrazolium chloride is a double tetrazole structure, forming purple
formazone product, helping in the detection and approximation of phagocytic
ability of the splenocytes.
FACS (Fluorescence Activated Cell Sorter) analysis was
performed on the isolated splenocytes. It’s a latest flowcytometric technique, which
is very helpful in the observation of physical characteristics of the cells. The cell size
can be studied by the FACS analysis and even the granularity of the isolated
splenocytes can be studied by performing the FACS analysis of the isolated
splenocytes.
All the above-mentioned experiments were performed on the two
different groups of mouse. One was normal Swiss albino mouse and the other was
induced with busalfan for the comparative study.

2. ACKNOWLEDGEMENT
I am highly grateful & pay my deep regards to our guide Dr.
Samaresh Chaudhuri, Professor & Head of the Department of
Biochemistry & Medical Biotechnology, School of Tropical Medicine,
Kolkata.
I am also thankful to Dr. Sujata Law, Assistant Professor, Dept of
Biochemistry & Medical Biotechnology,Calcutta School of Tropical
Medicine, Kolkata.
I am thankful to Dr.Biduyt Bandopadhay,head of the department of
Oriental Institute Of Science & Technology,Dr.N.K Sinha,Dr.Gaurab
Roy & others for their kind help.
Thanks to all my lab seniors especially Sumanta Chatterjee, Pratima
Basak, Prasun Das, Ranjan kr. Dutta & others who have always
guided me to do my work.
I would also like to thank my co-worker.
Last but not least, I would also like to express my heart felt gratitude
to my respected parents, who have been all along a co-assistant
source of inspiration throughout my project.
-Kamalika saha
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