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Last week you were to perform an artificial transformation experimen.pdf

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The document describes an artificial transformation experiment using an XL1-Blue II bacterial culture with an optical density (OD) of 0.68. To calculate the total number of cells: the OD of 1 equals 1x108 cells/ml, so an OD of 0.68 equals 6.8x108 cells/ml. The document asks how many total cells were in the cell pellet after spinning down 1.5ml of this culture in an Eppendorf tube.

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Last week you were to perform an artificial transformation experiment using 1.5ml of XL1-Blue II culture. Assume you were repeating this experiment and the OD of the XL1-Blue II culture was 0.68. a. What is the concentration of this culture (OD = 1x108cells/ml) My Answer: 6.8x108 cells/ml This is correct. b. How many cells total did you have in the cell pellet after spanning down your Eppendorf tube? Please show how to answer B

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