Localization of Exo70ΔdC-GFP (Exo70 lacking an internal domain C, one of 4 domains of the protein) relies on the actin cytoskeleton. (A), Exo70ΔdC-GFP localizes to sites of secretion in cells treated with DMSO but shows a diffuse localization pattern in cells treated with 100 μM LatA dissolved in DMSO. Exo70-GFP has a polarized localization in cells treated with DMSO or LatA. (B), The graph shows quantitation of Exo70ΔdC-GFP and Exo70-GFP localization from at least 100 cells for each condition.

1. A DMSO LatA
exo70ΔdC
EXO70
B
120
100
% cells with polarized GFP signal
80
UNBUDDED
60
SMALL BUDS
MEDIUM BUDS
40
LARGE BUDS
20
0
EXO70, EXO70, exo70ΔdC, exo70ΔdC,
DMSO LatA DMSO LatA
-20