VIP Call Girls Service Saharanpur Aishwarya 8250192130 Independent Escort Ser...
Digital Lab 9
1. Aerobic Metabolism: Evolution of a
New Energy-Producing Pathway
Digital Lab Report #9
By: Hadi Makkawi,
Ziad Toqan, and Aziz
Al-Harbi
quot;
2. Purpose…
Three Key Purposes of the Lab:
1. To be able to list the parts of the mitochondrion and describe
its activities in aerobic respiration
2. Discuss the mitochondrion as a product of endosymbiosis.
3. Summarize the products and by-products of aerobic
metabolism.
3. Procedure A: Oxygen
Consumption
1. First we filled a test tube with 10 germinating peas and placed a
loosely-packed cotton plug above the peas with some air space in
between. On top of the cotton plug we inserted 10 pellets of potassium
hydroxide (KOH).
2. Then, we inserted a respirometer with a drop of indicator fluid in
place. We then waited approximately 5 minutes before taking any
readings to make sure all was accurate.
3. Third, we placed 10 dry peas in another test tube and inserted the
respirometer the same way as before.
4. We recorded the position of the fluid droplet in both respirometers
every three minutes for half an hour.
4. Procedure B: Carbon Dioxide
Production
1. For the second part of the procedure we repeated the
steps in procedure one except this time we left out the KOH.
2. We then noted the position of the fluid droplet in both
respirometers every three minutes for half an hour.
6. Analysis of Recorded Data…
Analysis of recorded data:
As we can observe from the data recorded, KOH does indeed absorb
carbon dioxide. Through this experiment, we were able to see that when
KOH was added to the test tube filled with germinating peas the level of
oxygen consumption decreased before reaching a stabilizing. In the case of
dry peas, when the KOH was present the oxygen consumption of the peas
also saw a decline until reaching a stable level.
For part B, the test tube with germinating peas excluding KOH saw an
increase in carbon dioxide production until the middle until it slightly
dipped. This however was a mistake on our part as we tampered with the
respirometer. The intended outcome should have showed continued
increase in CO2 production. As for the tube filed with the dry peas
excluding the presence of KOH, CO2 production increased slightly until
finally reaching a stable level of 0.6.
8. General Analysis
The table and graph presented inthe earlier slides display the oxygen
consumption and carbon dioxide production in two different
situations. The first is of KOH in the presence of germinating peas
and dry peas, and the second without any addition of KOH to tubes
of germinating peas and dry peas. The experiment more or less shows
that the carbon dioxide production was somewhat approximately
equal to that of the oxygen consumption in aerobic respiration. Since
KOH is highly basic, it forms strong alkali solutions in water and
other polar solvents. This information presented through the data
comes to verify the initial hypothesis that KOH absorbs carbon
dioxide, and that is why when the KOH was removed from the setup,
we saw carbon dioxide production levels increase. And when KOH
was present in the germinating peas solution, we saw a decrease in the
oxygen consumption levels.
9. Conclusion...
This lab contributes to one of the key goals of the course as it
informs its lab conductors of the very idea that the evolution of
metabolic pathways such as aerobic respiration, and their merging
into major lineages of organisms, may have permitted life as we
know it to survive through evolutionary time. This can be seen as
significant as the evolution of aerobic metabolism, which is
considered a metabolic pathway that allows large amounts of ATP
to be produced with the presence of oxygen, as a crucial event in
the history of evolution. It is what was needed in order for multi-
cellular organisms to evolve and develop, which need higher
amounts of energy than unicellular anaerobic bacteria.
