1. The first handmade computer-stirred tank bioreactor, using an agitator-converted AMD ventilator
for plant cell and microorganism cultivation.
Abstract: In recent years, a couple of bioreactor modifications have been developed and successfully adopted for the in vitro cultivation of plant cells and microorganisms. Bioreactors such
as mechanically agitated, airlift and photobioreactors have been designed and used for large scale cultivation of algae, higher biomass and bioactive compounds production. Our primary
objective of this study was to build and design a cost effective and ecofriendly stirred tank bioreactor 4L, where the agitation rate (i.e. 150, 250, 500 rpm) would be controlled through computer
software without affecting the personal computer performance (temperature, graphics). As a result we have designed and developed the first computer in the world that is capable to yield
more than a simple computer (fermentation). This project aims to help every person in the world, who has access to a desktop computer, to accomplish similar achievements. Furthermore,
due to our friendly stance towards environment, we have manufactured our computer tower, using Plexiglas, a material easy to break down into its original components and completely
recycled.
Key words: AMD ventilator, stirred tank bioreactor, computer, bioactive compounds, computer software, Plexiglass, ecofriendly.
1. INTRODUCTION:
The concept of continuous culture dates back to 19th century, when a continuous process for the conversion of waste beers and wines to vinegar was developed. The chemostat invented in
the early ‘40's marked the advent of serious continuous fermentation. The use of continuous stirred tank reactor (CSTR) to extend duration of culture of microbes was developed in the ‘50s by
Novick, Szilard and Monod. The realization that a CSTR could be used to maintain microbial growth at a steady state value, that could be varied from any growth rate was an important
advance. It surpassed the traditional thinking at a time when stable microbial growth was only possible at the maximum rate, corresponding to the minimum doubling time found in batch
cultures. Bioreactors due to presence of mechanical pumps require high power consumption. As a result in the 21st century, we are able to introduce the most useful tool, a computer-
fermenter without additional energy costs.
4. DISCUSSION:
Some of the bioreactor’s pros:
Extremely compact than any bioreactor system on the market e.g 100 Liter QVF glass portable used agitated
tanks cost 2276.66 Euro, in contrast to our bioreactor, which costs about 20 Euro (excluding the
motherboard).
Despite of its compact size, the bioreactor allows a perfect visible access from all sides!
Does not require additional energy, because the primary circuit has been converted, without affecting
computer performance.
Weighs only 6 kg.
It is portable.
It is recyclable.
5. REFERENCES:
1) Agarwal, P., and Lim, H.C. (1984) Analyses of various control schemes for continuous bioreactors. Advances In Biochemical Engineering/Biotechnology, 30, 61-90.
2) Miller, Paul (2006-07-08). "Apple sneaks new logic board into whining MacBook Pros". Engadget. Retrieved 2013-10-30
3) Mueller, Scott 2005. Upgrading and Repairing PCs. Que Publishing. 16th edition. pp 1274–1280
4) Arpaci-Dusseau, Remzi H.; Arpaci-Dusseau, Andrea C. (2014). "Operating Systems: Three Easy Pieces [Chapter: Hard Disk Drives]" Arpaci-Dusseau Books.
5) "SpeedFan - Access temperature sensor in your computer. Almico.com
6) Encyclopedia of Packaging Technology, Third Edition by Kit L. Yam
2. METHOD
AND MATERIALS
1.1.Motherboard Asus
2V-
1.2.Ventilator
1.3.HDD seagate
1.4.SpeedFan 4.51
1.5. Stainless steel coil
1.6.Glass Jar
3. DEFINITIONS:
1.1. Motherboard is one of the most essential parts of a
computer system. It holds together many of the crucial
components of a computer, including the central
processing unit (CPU), memory and connectors for input
and output devices.
1.2. CPU fan. Used to cool the CPU (central processing
unit) heatsink. Effective cooling of a concentrated heat
source such as a large-scale integrated circuit requires a
heatsink, which may be cooled by a fan; use of a fan
alone will not prevent overheating of the small chip.
1.3. HDD. A hard disk drive is a data storage device
used for storing and retrieving digital information using
one or more rigid ("hard") rapidly rotating disks
(platters) coated with magnetic material.
1.4. SpeedFan is a program that monitors voltages, fan
speeds and temperatures in computers with hardware
monitor chips.
1.5. Stainless steel coil, a form of steel coil, containing
chromium, resistant to tarnishing and rust.
1.6. Glass Jar 4L.
.
Preparation and
sterilization of culture
media. 15 psi, 121° C
for 15
Inoculate with
preferable
microorganism in
laminar air flow
chamber to avoid
contamination.
• CCP2
Power on the computer
to manipulate agitation
rate through SpeedFan
software
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Main window
A. Miron2, G. Kuziortis2, and T.P. Zafiriadis1
1 DVM-Food Technologist MSc, Professor of Katerini’s Public Educational Training Institution, Director of ESTIA LAB IKE
2 Undergraduate Students, Technological Educational Institution of Thessaly Contact: arismirons@gmail.com
• CCP1