health

1. Evaluation of anti-psoriatic activity of selected
phytochemicals on UV-induced psoriasis in mouse tail
model
Presenting Author details
Name-Dr.Jada Naga Lakshmi
Designation-Associate Professor
Name & Address of Organization
Vignan Pharmacy College,
vadlamudi-522213.
09-04-2023 1
Presentation Code-VPCV

2. Aim and objective
09-04-2023 2
Anti-psoriatic activity of selected phytochemicals on UV-induced psoriasis in
mouse tail model
 Hesperidine
 liquorice
 Ellagic acid
 Geraniol

3. METHODOLOGY
• Selected phytochemicals were purchased from Sigma Aldrich
• Conducted the phytochemical analysis for testing purity of
phytochemical
• Procuring of animals from after getting IAEC approval
• Acclimatise the animals to our environment by keeping it on
quarantine
• Daily contact of animal to UV-Radiation, for inducing psoriasis
09-04-2023 3

4. METHODOLOGY
 Mice of both sexes were taken and all groups of animals
 Those groups treated as group-1(vehicle control), group -2(disease control) group -3
(standard) group-4 (1%w/w) group-5(2%w/w) of respective test compound.
 Salicylic acid ointment was applied to mouse tail of standard group
 Remaining group animals were treated with respective test compound at two
concentrations. This process is continued for 21 days.
 Later mice’s were sacrificed; tails were collected and sended to histopathological
studies.
09-04-2023 4

5. RESULTS & DISCUSSION
Evaluation of anti psoriatic activity of test compounds
Epidermal thickness of mice tail was determined to observe the severity of psoriasis, physical
observation of animal skin including erthyma, itching and slivery patches on tail region.
Psoriasis severity score index was calculated for each group to compare the efficacy of test
compounds with that of standard. Psoriasis area and severity index (PSI) scores were determined by
evaluating the degree of erythema, thickening, and scaling on the affected dorsal skin surface.
PSI for each was measured on a four-point scale (0 = none; 1 = slight; 2 = moderate; 3 = marked; 4 =
very marked). The severity of skin inflammation was measured by the combined scores (erythema
plus scaling plus thickening) giving a range of scores of 0–12. Histological parameters are also
observed by collecting the tail skin of mice.
09-04-2023 5

6. RESULTS AND DISCUSSIONS
09-04-2023 6
S.No Treatment group
Epidermal thickness (mm)
Hesperdine Ellagic acid Liquorice Geraniol
1 Vehiclecontrol 7.2±1.3038 6.8±0.7999 6.8±0.3741 7.2±0.583
2 Disease control 10.4±1.1401 11.2±0.4898 11.8±0.7348 10.4±0.678
3 Standard 7.4**±1.1401 6.8**±0.3741 5.8*±0.3741 7.4**±0.509
4 1%w/w 9.8±2.3874 8.6±1.4352 10.8 ±1.7719 9.6 ***±0.927
5 2% w/w 10.4±1.8165 8*±0.8366 7.6**±0.6781 8.4****±0.748
Table .No.1. Effect of Phytochemicals on epidermal thickness of mouse tail in
UV-Induced psoriatic animals
Note: Values are Mean ± SEM; epidermal thickness was significantly decreased
than the disease control group, Data are analyzed by one -way ANOVA and two-way ANOVA (Dunnett's
multiple comparisons test) n=5, (**p< 0.05) (*** p< 0.001) (**** p< 0.0001)

7. RESULTS AND DISCUSSIONS
S.No Group 1 st week 2nd week 3rd week
1 Disease control 2.3 2.8 3.2
2 Vehicle control 0 0 0
3 Standard 2.1 1.5** 1.0**
4 Hesperidine 1% 2.1 1.7** 1.2**
5 Hesperidine 2% 2.3 1.6** 1.1**
6 Ellagic acid 1% 2.4 1.6** 1.2**
7 Ellagic acid 2% 2.3 1.3** 0.8**
8 Liquorice1% 2.2 1.9** 1.3**
9 Liquorice2% 2.4 1.7** 0.9**
10 Geraniol 1% 2.3 1.9** 1.1**
Geraniol 2% 2.4 1.7** 0.8**
09-04-2023 7
Table No.2 Effect of test compounds on Psoriasis Severity Index
Note: The results are obtain from one -way ANOVA, PSI ** p <0.05

8. RESULTS AND DISCUSSION
09-04-2023
International Conference on Current Trends in Drug Discovery
Development & Delivery (CTD4-2022),
KL College of Pharmacy
8

9. CONCLUSION
• Exposure to UV-Rays is the one of the major cause in number of people who
were suffering with psoriasis is due to directly or indirectly exposure to UV-light
in their regular life.
• This rays may induced the genetically changes in the kerationocytes which may
lead to the proliferation of keratinocytes in the skin.
• That can induce the psoriatic lesions in the skin. UV-induced psoriasis mouse tail
model is widely used to screen for the antipsoriatic activity of crude drugs
• The result of the study showed that the 2% of geraniol, liquorice and ellagic acid
exhibited significant activity on UV- induced psoriasis in rodents.
• The study implies that selected phytoconstituents are a promising research for
further investigations to prove its anti-psoriatic activity.
09-04-2023 9

10. THANK YOU
09-04-2023 10