Physical Methods of Sterilization

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Sterilization is the complete removal of microorganisms from an object or surfaces. Sterilization is obtained when microorganisms are subjected to antimicrobial agents for sufficient time and at optimum conditions. Sterilization is a process of eradicating live microorganisms from substances. It is done to preserve things for a long time and kill germs. If something is not sterilized, it may cause infection to those who use it. Therefore, it should not be taken for granted.

Science

PHYSICAL METHODS OF
STERILIZATION
FILTRATION: TYPES OF FILTERS & LAMINAR AIR FLOW
22nd March, 2019
Presented by,
SHRYLI K S
IVth Semester
Molecular Biology
YCM (Autonomous)
Guided by,
Dr N S Devaki
Associate Professor
Dept. of Molecular Biology
YCM (Autonomous)

CONTENTS
• INTRODUCTION
• FILTRATION
• MEMBRANE FILTERS
• LAMINAR AIR FLOW
• BIOHAZARDS SAFETY LEVELS OF LAMINAR AIR FLOW
• CONCLUSION
• REFERENCES
• ACKNOWLEDGEMENT

TYPES OF FILTERS
• Candle filters
• Asbestos filter
• Sintered glass filter
• Membrane filters
• Syringe filter

ASBESTOS FILTER
Fig 2: Asbestos filter
Fig 3: Cross section of an
Asbestos filter

SINTERED GLASS FILTER
Fig 4: Sintered glass filter Fig 5: filtration using sintered glass filter

MEMBRANE FILTERS
Mixed Cellulose Ester Cellulose Acetate Coated Cellulose Acetate
Nylon Filter
Polycarbonate Filter
Fig 6: Membrane filters

LAMINAR AIR FLOW
Fig 8: Laminar Air Flow Fig 9: Air Flow Movement in
Laminar Air Flow

BIOHAZARD SAFETY LEVELS OF
LAMINAR AIR FLOW

REFERENCES
• Joanne M Willey, Linda M Sherwood & Chirstopher J Woolverton. Prescott’s
Microbiology. 9th edition. The McGraw-hill companies publication. 1221 Avenues,
New York, 2011, 1139 pages.
• R Ananthanarayan, C K Jayaram Panikar, Textbook of Microbiology. Orient Longman
Private Limited, Hyderabad, 2000, Sixth edition.
• http://www.advantecmfs.com/catalog/filt/membrane.pdf
• https://ehs.missouri.edu/ehs/bio/labprac/biocabinet
• www.rpi.edu

ACKNOWLEDGEMENT
I thank the department of Molecular Biology for giving me an opportunity to
present my seminar. I would also like to thank my guide Dr N S Devaki
Ma’am for her valuable guidance and support.
Thank you all.

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Molecular markers are identifiable DNA sequences used to locate genes associated with specific traits or genetic conditions. A molecular marker is a specific gene fragment present at a specific position called ‘locus’ (pleural loci) in the genome of a cell. In the pool of unknown DNA or in a whole chromosome, these molecular markers help in identification of particular sequence of DNA at particular location.

Molecular markers- RFLP, RAPD, AFLP, SNP etc.

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Physical Methods of Sterilization

1. PHYSICAL METHODS OF STERILIZATION FILTRATION: TYPES OF FILTERS & LAMINAR AIR FLOW 22nd March, 2019 Presented by, SHRYLI K S IVth Semester Molecular Biology YCM (Autonomous) Guided by, Dr N S Devaki Associate Professor Dept. of Molecular Biology YCM (Autonomous)

2. CONTENTS • INTRODUCTION • FILTRATION • MEMBRANE FILTERS • LAMINAR AIR FLOW • BIOHAZARDS SAFETY LEVELS OF LAMINAR AIR FLOW • CONCLUSION • REFERENCES • ACKNOWLEDGEMENT

3. INTRODUCTION

4. FILTRATION

5. TYPES OF FILTERS • Candle filters • Asbestos filter • Sintered glass filter • Membrane filters • Syringe filter

6. CANDLE FILTER Fig 1: Candle Filter

7. ASBESTOS FILTER Fig 2: Asbestos filter Fig 3: Cross section of an Asbestos filter

8. SINTERED GLASS FILTER Fig 4: Sintered glass filter Fig 5: filtration using sintered glass filter

9. MEMBRANE FILTERS Mixed Cellulose Ester Cellulose Acetate Coated Cellulose Acetate Nylon Filter Polycarbonate Filter Fig 6: Membrane filters

10. SYRINGE FILTER Fig 7: Syringe Filter

11. LAMINAR AIR FLOW Fig 8: Laminar Air Flow Fig 9: Air Flow Movement in Laminar Air Flow

12. BIOHAZARD SAFETY LEVELS OF LAMINAR AIR FLOW

13. CONCLUSION

14. REFERENCES • Joanne M Willey, Linda M Sherwood & Chirstopher J Woolverton. Prescott’s Microbiology. 9th edition. The McGraw-hill companies publication. 1221 Avenues, New York, 2011, 1139 pages. • R Ananthanarayan, C K Jayaram Panikar, Textbook of Microbiology. Orient Longman Private Limited, Hyderabad, 2000, Sixth edition. • http://www.advantecmfs.com/catalog/filt/membrane.pdf • https://ehs.missouri.edu/ehs/bio/labprac/biocabinet • www.rpi.edu

15. ACKNOWLEDGEMENT I thank the department of Molecular Biology for giving me an opportunity to present my seminar. I would also like to thank my guide Dr N S Devaki Ma’am for her valuable guidance and support. Thank you all.

Physical Methods of Sterilization

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