Introduction of Ethosome, Methods of Preparation, advantage and Disadvantage, Application

1. ETHOSOMES
Presented By:
Saurabh sharma
2nd Semester
Pharmaceutics
School Of Pharmaceutical Sciences
Rajiv Gandhi Proudyogiki Vishwavidyalaya, Airport Road, Gandhi
Nagar, Bhopal
Session 2018- 2020
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2. Flow of Presentation
• Introduction
• Types of ethosomes
• Mechanism of ethosome
• Additives used in ethosome
• Method of preparation
• Characterization of ethosome
• Advantages and limitations
• Summary

3. Ethosomes are ethanolic liposomes
The ethosomes are vesicular carriers consisting of hydroalcoholic or
hydroglycolic phospholipids in which the concentration of alcohols or their
combination is relatively high.
These are soft, malleable vesicles tailored for enhanced delivery of active
agents.
Ethosomes which are novel permeation-enhancing lipid vesicles
embodying high concentration (20–45%) of ethanol
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7. Ethanol effect
 Ethanol acts as a penetration enhancer through the skin. Ethanol
penetrates into intercellular lipids and increases the fluidity of cell
membrane lipids and decrease the density of lipid multilayer of cell
membrane
Ethosomes effect
 Ethosomes permeates very easily inside the deep skin layers, where it
got fused with skin lipids and releases the drugs into deep layer of skin
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8. Contd….
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9. CLASS EXAMPLES USES
Phospholipid Soya phosphatidylcholine,
Egg phosphatidylcholine,
Dipalmityl phosphatidylcholine,
Distearyl phosphatidylcholine
Vesicles forming component
Polyglycol Propylene glycol, Transcutol As a skin penetration enhancer
Alcohol Ethanol, Isopropyl alcohol For providing the softness for vesicle
membrane, as a penetration enhancer
Cholesterol Cholesterol For providing the stability to vesicle
membrane
Dye Rhodamine-123, Rhodamine
Red, Fluorescene Isothiocynate
(FITC), 6- Carboxy fluorescence
For characterization study
Vehicle Carbopol Ð934 As a gel former
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10. Method of
Preparation
Hot method Cold method
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12. Disperse phospholipid
in water at 40˚C
Ethanol + propylene
glycol at 40˚C
Mix organic phase to
aqueous phase
Add Drug, which is
dissolved in suitable
solvent
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13. Parameter Importance Method
Size and Shape Determine skin penetration SEM, TEM, Dynamic
Light Scattering
Zeta potential Stability of vesicles Zeta Sizer
Entrapment
efficiency
Suitability of method Ultracentrifugation
and HPLC
Drug content Important in deciding the amount of vesicle
preparation to be used
UV, HPLC
Stability studies To determine the shelf life of vesicle formulation SEM, TEM, HPLC
In vitro dissolution Determine the drug release rate from vesicle Franz diffusion cell
Skin permeation Determines rate of drug transport through skin Confocal Laser
Scanning Microscopy
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14. Enhanced permeation of drug through skin for transdermal drug delivery
Delivery of large molecules (peptides, protein molecules) is possible
It contains non‐toxic raw material in formulation
Ethosomal drug delivery system can be applied widely in Pharmaceutical,
Veterinary, Cosmetic fields
Simple method for drug delivery in comparison to Iontophoresis and
Phonophoresis and other complicated methods
High patient compliance‐ The ethosomal drug is administrated in semisolid
form (gel or cream) hence producing high patient compliance
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15. DRUG APPLICATION ADVANTAGE
Minodixil Hair growth promotion Higher skin retention
Cannabidol Prevents inflammation and
edema
Significant accumulation
of the drug in the skin
Bacitracin Treatment of dermal
infections
Reduced drug toxicity
Acyclovir Treatment of Herpetic
infection
Improved drug delivery
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16. Ethosomal administration is not a means to achieve rapid bolus type drug
input, rather it usually designed to offer slow, sustained drug delivery
The molecular size of the drug should be reasonable that it should be
absorbed percutaneously
Adhesive may not adhere well to all types of skin
May not be economical
Poor yield
Skin irritation or dermatitis due to excipients and enhancers of drug
delivery systems
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18. The main limiting factor of transdermal drug delivery system is epidermal
barrier can be overcome by ultra deformable vesicles to significant extent
They are emerging vesicular carrier because they have site specificity, high
penetration power, higher deformability and higher stability
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