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Analysis of lisinopril by UV Sphectrometry
1. Development AND VALIDATION OF UV
SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION
OF LISINOPRIL IN BULK AND PHARMACEUTICAL
FORMULATION
SABYA SACHI DAS
PhD Scholar
Dept. Of Pharm. Sci. & Tech.
Birla Institute Of Technology
Mesra,Ranchi-835215,Jharkhand
2. introduction
Simple UV-Spectrophotometric methods was
developed for the determination of Lisinopril in
bulk and its pharmaceutical dosage forms.
Lisinopril exhibited maximum absorption at
241nm by UV spectroscopy using 0.1N NaOH as
the solvent.
The drug obeys linearity in the concentration
ranges of 5-60 g/ml.
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4. Chemical data:
Empirical formula : C21H31N3O5
Molecular weight : 405.5 gm/mole
Physical stability:
Colour : White to off-white.
Solubility : Soluble in water, sparingly soluble in
methanol, practically insoluble in ethanol.
Melting point : 146-148 ⁰C
Form : Crystalline powder
λ max : 241nm (uv)
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5. Pharmacological action:
Lisinopril inhibits angiotensin converting enzyme (ACE) in
human subjects and animals. ACE is a peptidyl dipeptidase
that catalyzes the conversion of angiotensin I to the
vasoconstrictor substance, angiotensin II.
Angiotensin II also stimulates aldosterone secretion by the
adrenal cortex. The beneficial effects of lisinopril in
hypertension and heart failure appear to result primarily from
suppression of the renin-angiotensin- aldosterone system.
Inhibition of ACE results in decreased plasma angiotensin II
which leads to decreased vasopressor activity and to
decreased aldosterone secretion.
Category : ACE INHIBITOR (Anti hypertensive drug)
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6. LITERATUREREVIEWON LISINOPRIL
The Intraday and Interday precision for Lisinopril and its
active metabolite were found to be 0.213±0.0028 and
0.204±0.0019 respectively.
Correlation co-efficient for Lisinopril was found to be 0.9994.
%RSD for lisinopril was found to be 1.314.
HPLC method was validated according to the guidelines of
Food and Drug Administration, showing to be accurate (bias
within ±12%)over the concentration range of 5-60 μg/mL for
Lisinopril.
Absorbance was monitored at 241 nm where Lisinopril has
maximum absorption.
Tablet dosage form was estimated, and percentage recovery
was 98.4-99.87%.
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11. Precision
The precision of the proposed method was
ascertained by actual determination of six
replicates of fixed concentration of the
drug within the Beer’s range and finding
out the absorbance's by the proposed
method.
From these absorbance's Mean, Standard
deviation and %R.S.D was calculated.
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13. ACCURACY
To determine the accuracy of the proposed
method, recovery studies were carried out by
taking different amounts (80%, 100% and 120%)
of bulk samples of Lisinopril within the linearity
range and adding to the pre-analyzed
formulation concentration.
From that percentage recovery values were
calculated.
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15. CONCLUSION
The proposed method is simple, sensitive and
reliable with good precision and accuracy.
The proposed method is specific while
estimating the commercial formulations without
interference of excipients and other additives.
Hence, this method can be used for the routine
determination of Lisinopril in bulk samples and
Pharmaceutical formulations.
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16. REFERENCE
GRChatwal, S Anand, Instrumental Methods of Chemical
Analysis; 2002:209-217.
Scottish intercollegiate guidelines network (sign), control of pain in
adults with cancer-a national clinical guideline. Scotland national
health service (nhs).2008:76-89.
G. T. Warner and B. Jarvis, Olmesartan medoxomil,
Drugs 62 (2002) 1345–1353.
Sissmann j, bouroudian m, armagnac c, donazollo y, latreille m, and
panis r. Angiotensin ii blockade in healthy volunteers: tolerability and
impact on renin angiotensin system components of single and
repeated doses of a new angiotensin ii receptor antagonist sr 47436
(bms 186295). J hypertens 1994; 12: s92.
Metabocard for lisinopril retrieved 29 april 2004: 1010-
1025.
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