Comprission of swelab & hemocue (1)

‫الرحيم‬ ‫الرحمن‬ ‫هللا‬ ‫بسم‬‫الرحيم‬ ‫الرحمن‬ ‫هللا‬ ‫بسم‬
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COMPRESSION OF SWELAB HEMATOLOGY ANALYSER WITH HEMOCUE FOR TWBCs & DIFFERENTIAL:
Note: I used her swelab analyzer because it the stander analyzer I use it in my lab and I use to prepair monthly sample forhemoglobin andhematology analyzer moinetaring to allKhartoum state hospital and it give agood tolerance result
.and alsogive excellent result with the riqase external progrmme by randox (UK).
1- GENERAL CHARACTERESTIC: (TABLE1)
NAME OF
INSTRUMENT
ORIGINE PARAMETE
ESTMATED
PRINCIPLE NO OF
REAGENT
USED
NO OF TEST
DON BY
ONE SET OF
REAGENT
TYPE OF
TECHNOLOGY
LCD
SCREEN
PRINTER
SWELAB SWEDEN 20 PARAMETRE
WITH 3 WBCs
HISTOGRAMME
ELECTRICAL
IMPEDANCE
TECHNIQUE
2 WITH
CLEANING
SOLUTION
EVERY 2000
SAMPLE
900
SAMPLE
3PART
DIFFERENTIAL
WITH 1
FLOATING
DISCRMINATOR
TOUCH
COLORED
SCREEN
EXTERNAL
PRINTER
OVER WITH
THE
INSTRUMENT
HEMOCUE SWEDEN TWBCs +
DIFFERENTIAL
OF 5 WBCs
POPULATION
CYTOCHEMICAL
STAIN USING
CAMERA TO
SELECT THE
IMAGE
ONE SINGLE
DISPOSABLE
CASSETTE
FOR EACH
TEST
EVERY TEST
NEED
SINGLE
CASSETTE
TWBCs + 5
PART
DIFFERENTIAL
COLOR
SCREEN
NO PRINTER
*Swelab major principle: based on the aperture impedance principle in which blood cells which are non-conductors of electricity are
diluted in a buffered electrolyte solution and allowed to pass through the orifice of an aperture tube between two electrodes Interruption of
the current by the non-conducting cells alters the electric charge and a pulse is produced. The amplitude ofeach pulse is proportional to the
volume of the cell and the cell count is determined from the total number of pulses obtained from a measuredvolume of blood.
*Hemocue major principle: drop of blood - 10µL – from a finger stick or venous sample. In the microcuvette, red cells are lyzed by an
hemolyzing agent and white cells are stained by methylene blue. The camera in the analyzer takes 37 images throughout the cuvette, and the
cells are counted by image analysis and classified intoeach subgroup. A lab accurate result is achieved within less than five minutes.

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2- METHODICAL CHARACTERASTIC:
*1- We used fresh EDTA blood sample from 40 different normal and abnormal person collected on standerd mannar ,the test is done
immediately after blood collection or during the time period not exceeding two hour from blood collection .
*2-The abnormal persons usedin this evaluation have pathological abnormality 12 of them are sickel cell anemia ,8 have shift to left
leucocyte mainly myeloid cells .
*3- The others 20 persons are healthy normal male and femal .
*4 – We use the following formulas to calculate the different WBCs parameter to obtain the performance relation between the two
instrument.
A We calculate the total measured sample by summation of the 40 sample.
B Calculate the average mean by dividing the total measure sample by 40.
C substeract the mean of 40 sample read by hemocue from 40 sample read by swelab to obtain the absolute error of hemocue in relation to
swlab.
D The correlation coefficient to determine the relationship between two instrument.
E Covariance, the average of the products of deviations for each data point pair. Use covariance to determine the relationship between two
instrument data .
F Intercept Calculates the point at which a line will intersect the y-axis by using existing x-values and y-values.The intercept point is based
on a best-fit regression line plotted through the known x-values and known y-values. Use the INTERCEPT function when you want to
determine the value of the dependent variable when the independent variable is 0 (zero).
G The precession her we use the repreducability (within run) to see the degree of measurement agreement when the same sample is
repeat at the same time by the same person and the same manner . we repeat fresh EDTA blood sample collect in standerd way for ten time
and calculate standerd deviation (SD) and coefficient of variation (C.V).
H We plot the the data obtain usining XY scatter chart and culomn and line chart.

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(TABLE 2)
METHODICAL CHARACTERASTIC
description TWBCs NEUTROPHIL LYMPHOCYTE MIX
0 TYPE OF
INSTRUMENT
SWELAB HEMOCUE SWELAB HEMOCUE SWELAB HEMOCUE SWELAB HEMOC UE
1 SUMOF READING 377.4 415.7 2121.8 1976 1580.8 1602 297 402
2 AVERAGE OF
READING
9.435 10.3925 53.045 49.4 39.52 40.05 7.425 10.575
3 DIFERANCE OF
READING(ABSOLUTE
ERROR)
0.9575 3.645 0.53 3.15
4 CORELATION 0.968573 0.836793 0.824329 0.240006
5 COVARIENT 27.33026 134.727 131.5865 3.743125
6 INTERCEPT 1.292691 0.240225 0.496402 6.028478
7 Precision(repeatability
test) for hemocue
device
5.635 % 5.799 % 5.084156 %

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3- STATISTICAL CHART
SERIES 1 = SWELAB
SERIES 2 = HEMOCUE
No (1) Charts for TWBCs COUNT 3 chart
* CORELATION BETWEEN SWELAB & HEMOCUE TWBCs MEASURMENT IS : 0.968573
0
5
10
15
20
25
0 10 20 30
AxisTitle
Axis Title
TWBCs XY SCATTER CHART
S
e
ri
e
s
0
5
10
15
20
25
1 4 7 10 13 16 19 22 25 28 31 34 37 40
TWBCs CULOMN CHART
Series1
Series2
0
5
10
15
20
25
1 4 7 1013161922252831343740
AxisTitle
TWBCs LINE CHART
Series1
Series2

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* CORRELATION BETWEEN SWELAB & HEMOCUE Neutrophils MEASURMENT IS : 0.836793
NO (2) CHART FOR NEUTROPHIL DIFFERENTIAL 3 CHART
0
20
40
60
80
100
1 4 7 10 13 16 19 22 25 28 31 34 37 40
AxisTitle
NEUTROPHILLINE CHART
Series1
Series2
0
50
100
1 5 9 13 17 21 25 29 33 37
NEUTROPHILCULOMN
CHART
Series1
Series2
0
20
40
60
80
100
0 50 100
AxisTitle
Axis Title
NEUTROPHILXY SCATTER
CHART
S
e
r
i
e

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NO (3) CHART FOR LYMPHOCYTE DIFFERENTIAL 3 CHART
* CORRELATION BETWEEN SWELAB & HEMOCUE Lymphocyte MEASURMENT IS : 0.824329
0
20
40
60
80
1 4 7 10 13 16 19 22 25 28 31 34 37 40
AxisTitle
LYMPHOCYTELINE CHART
Series1
Series2
0
20
40
60
80
1 4 7 10 13 16 19 22 25 28 31 34 37 40
LYMPHOCYTECULOMN
CHART
Series1
Series2
0
50
100
0 20 40 60 80
AxisTitle
Axis Title
LYMPHOCYTEXY SCATTER
CHART S
e
ri
e
s
1

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NO (4) CHART FOR MIX CELL (ESINOPHIL,MONOCYE , BASOPHIL) DIFFERENTIAL 3 CHART
* CORRELATION BETWEEN SWELAB & HEMOCUE Mix Cell MEASURMENT IS: 0.240006
0
10
20
30
40
0 5 10 15
AxisTitle
Axis Title
MIX CELL XY SCATTER
CHART S
e
ri
e
s
1
0
10
20
30
40
1 5 9 13172125293337
AxisTitle
MIX CELL LINE CHART
Series1
Series2
0
5
10
15
20
25
30
35
40
1 4 7 10 13 16 19 22 25 28 31 34 37 40
MIX CELL CULOMN CHART
Series1
Series2

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4- Conclusion:
*1-The hemocue device is small in size and take small area within the lab.
*2- The test almost take about three minute to 5 minute (by hemocue) to obtain the result .
*3- I notifY that when ever theiris abnormal WBCs or high TWBCs the value of obtained result will give high dispersion between the hemocue
and swelab analyzer .witch mean it use only in normal population or only reactive leucocyte to certain infection (bacterial or viral) and parasitic
or ellergic response and not suitable to be usedin area of suspected presence of patientwith abnormal pathological WBCs change.
*4-The correlation between the swelab analyzer and the hemocue is highly agreed when the TWBCs is normal andless than 15000 / cumm
*5- The Linearity of the hemocue device is(about 30000 cell/ cumm) and thiswas obvious when I use sample of more than 100,000 cell/cuumm
witch give alarm of( HHH) when I dilute the sample with citrate phosphate dextrose adenine one (CPDA1) by 3 time it give me flage N1 . when I
use other sample of 21000 it give the same or almost near to the reading of swelab.
*6-Hemocue device is highly sensitive to the sample integrity and storage condition and the way of cassette fillingto obtain the result.
*7-The precision (REPEATABILITY) is slightly high more than 5%.
*8-For hemocue I summated the esinophil &monocyte &basophil to meet with the three part differential of swelab analyzer.
RESULT: -
*Hemocue is point of care instrument use toscreen for only normal TWBCs with it is normal differential.
NAZAR AHMED MOHAMED ABD-ALLA
BSC: HEMATOLOGY & BLOOD TRANSFUSION MEDCINE
HIGH DIPLOMA IN HEMATOLOGY 13/06/2013

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