1. Nanostructured Surfaces:
Development of a New MS-Platform to Capture,
Process and Analyse (CPA-MALDI)
Urine-Derived Biomolecular Compounds
by
Monica Epifanio (monica.epifanio@dcu.ie)
Marie Curie fellow at Tethis S.p.A (Milan, Italy) and Dublin City University (Dublin, Ireland)
SHOTGUN PRESENTATION
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2. To develop a new diagnostic technology based on:
 Smart Nano-structured surfaces;
 Suitable for MALDI -MS;
 Micro-fluidics.
Aim of my work
Key challenges in proteomics-based
clinical diagnostics
 Continuous need and wish for higher sensitivity
 Ability to analyse complex samples
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3. Our solution…
Nanoparticle Beam Deposition system
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4. UNWASHED WASHED
UNWASHED WASHED
ID=2 mm
6 microliters
Sample concentration and capturing
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NS-COATED PLATE

5. MSI Mass Spectrometry Imaging
VI. Data processing
III. MALDI analysis
V. Image reconstruction
II. Transfer on ITO+TiO2 slide and matrix depositionI. Sacrifice and organ dissection
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6. http://www.tethis-lab.com/
PEPTIDES
PROTEINS
Trypsin digestion
CPA-MALDI
2D
Capturing-Processing-Analysing
1D
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7. Acknowledgment
Dr. Emanuele BarboriniDr. Marc BaumanDr. Alexander YavorskyyMr. Simone Vinati
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Mr. Giacomo Bertolini

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