6. General Principle:
• Physical properties, such as size (represented by forward angle light scatter) and
internal complexity (represented by right-angle scatter) can resolve certain cell
populations.
•Information displayed in Histogram and Dot-Plot scatter
•Cells can be sorted with the help of FS and SS
7. Histogram Dot Plot Scatter
http://www.labome.com/method/Flow-Cytometry-A-Survey-and-the-Basics.html
How will you Analyze ?
9. APPLICATION:
Research
• DNA content analysis
Aneuploidy
Gross gains/loses
• Protein expression and localization
• Chromosome analysis and sorting
• Protein engineering
Yeast display
Bacterial Display
• Resolving certain origin of gliomas