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Foregene presentation-better life science
- 1. Foregene and its Life Science Reagent
- 2. CATALOGUE 1 2 3 4 5 R&D Strength Foregene’s future Quality Control Products and application Company Introduction
- 3. • Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. • Foregene currently has world’s leading Direct PCR technology platform, DNA-only silica membrane technology platform, and LncRNA research one-stop technology service platform. The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. • The technology and product performance indicators used are all leading the world status, widely used in basic research fields of life sciences, medicine, agriculture, pharmacy, environment, etc, welcomed by hospitals, institutions, medical laboratories, etc. Company Introduction
- 4. Key Figures Team of 30 （PhD and master） 2500㎡ office building 600㎡ P2 laboratory 1200㎡ GMP workshop 2015 Sichuan Province "Thousand Talents Plan" entrepreneurial team 2018 Selected as the entrepreneurial team of Chengdu "Rongpiao Project"(funded by RMB 5 million)
- 5. Platforms • With special technology, the nucleic acid needn’t be extracted, just do PCR directly. Direct PCR technology platform • The RNA-Only Column and DNA-Cleaning Column was adopted to achieve rapid purification of genomic DNA without DNase, high- quality total RNA can be isolated. DNA-only silica membrane technology platform • Used in screening and identification, target identification, functional research. LncRNA research one- stop technology service platform
- 6. Product Series Life science product Series Features RNA Purification Quickly and efficiently extract high-purity and high-quality total RNA from various animal tissues,cells, plants, and virus, etc. DNA Purification Rapid extraction and purification of genomic DNA from animal, blood, bacteria,plant,mouse tail,water,stool, etc. PCR/RT-PCR Easily and efficiently synthesize high-quality first-strand cDNA,simple and fast operation of PCR and RT-PCR Direct PCR Directly use sample lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. Such as animal tissue,mouth tail, zebra fish,blood,plant leaf,plant seed,etc. IVD kits SARS-CoV-2 Nucleic acid detection kits by multiplex fluorescent PCR method, for detection of ORF1ab, N, E,S genes, flu A and B, etc. Lab equipment and consumables PCR machine, real time PCR system, and more to come.
- 7. Product Catalogues Plasmid DNA purification,DNA fragment purification and recovery, genomic DNA purification series Series Product Name Descriptions Specifications Catalogue Number Storage Conditions Plasmid DNA purification General plasmid mini-extraction kit General Plasmid Mini Kit Quickly purify high-quality plasmid DNA from transformed bacteria for routine molecular biology experiments such as transformation and enzyme digestion 50 Preps DE-01001 Short-term room temperature, long-term 2-8℃ DNA fragment purification and recovery Gel Extraction Kit Gel Extraction Kit Quickly and efficiently recover 20bp-10kb DNA fragments from agarose gel 50 Preps DE-02011 Short-term room temperature, long-term 2-8℃ PCR Purification Kit PCR Purification Kit Quickly purify and obtain high-quality DNA fragments from the PCR system; if you want to separate and obtain specific DNA fragments, please choose a gel recovery kit 50 Preps DE-03011 Short-term room temperature, long-term 2-8℃ Genomic DNA purification series Animal Tissue DNA Isolation Kit Animal Tissue DNA Isolation Kit Rapid extraction and purification of genomic DNA from multiple sources, such as animal tissues, cells, etc. 50 Preps DE-05011 Protease 4℃, other room temperature Blood DNA Mini Kit(<1ml) Blood DNA Mini Kit(<1ml) Quickly purify high-quality genomic DNA from anticoagulated blood (<1ml) 50 Preps DE-05111 Protease 4℃, other room temperature Blood DNA Midi Kit(1-5ml) Blood DNA Midi Kit(1-5ml) Quickly purify high-quality genomic DNA from anticoagulated blood (1- 5ml) 50 Preps DE-05121 Protease 4℃, other room temperature Mouse Tail DNA Mini Kit Mouse Tail DNA Mini Kit The world's fastest kit for extracting mouse tail genomic DNA, which can extract high-quality genomic DNA from mouse tail within 1 hour 50 Preps DE-05211 Protease 4℃, other room temperature Bacterial DNA Isolation Kit Bacterial DNA Isolation Kit Quickly purify high-quality genomic DNA from bacteria in the logarithmic growth phase 50 Preps DE-05311 Protease 4℃, Lysozyme -20℃
- 8. Product Catalogues Plasmid DNA purification,DNA fragment purification and recovery, genomic DNA purification series Series Product Name Descriptions Specifications Catalogue Number Storage Conditions Genomic DNA purification series FFPE DNA Isolation Kit FFPE DNA Isolation Kit Quickly purify high-quality genomic DNA from paraffin-embedded tissues such as paraffin sections and paraffin blocks 50 Preps DE-05411 Protease 4℃, other room temperature Soil DNA Isolation Kit Soil DNA Isolation Kit Quickly purify and obtain high-quality genomic DNA from various soil sample microorganisms 50 Preps DE-05513 Protease 4℃, Lysozyme -20℃ Water DNA Isolation Kit Water DNA Isolation Kit Quickly purify high-quality genomic DNA from microorganisms in various water samples 50 Preps DE-05613 Protease 4℃, Lysozyme -20℃ Stool DNA Isolation Kit Stool DNA Isolation Kit Quickly purify and obtain high-quality genomic DNA from various stool samples 50 Preps DE-05713 Protease 4℃, Lysozyme -20℃ Buccal Swab（FTA Card）DNA Isolation Kit Buccal Swab（FTA Card）DNA Isolation Kit Quickly purify high-quality genomic DNA from buccal swab samples 50 Preps DE-05811 Protease 4℃, other room temperature Plant DNA Isolation Kit Plant DNA Isolation Kit Quickly purify and obtain high-quality genomic DNA from plant samples (including polysaccharides and polyphenol plant samples) 50 Preps DE-06111 Protease 4℃, other room temperature Genomic DNA Micro Kit Genomic DNA Micro Kit Quickly purify small samples to obtain high-quality genomic DNA 50 Preps DM-01011 Protease 4℃, other room temperature RNA purification series Animal miRNA Isolation Kit Animal miRNA Isolation Kit Quickly and efficiently extract small RNA fragments of 20-200nt miRNA, siRNA, snRNA from various animal tissues and cells 50 Preps RE-01011 Room temperature 200 Preps RE-01014 Serum(Plasma) miRNA Isolation Kit Serum(Plasma) miRNA Isolation Kit Fast and efficient separation and purification of miRNAs with a length of less than 100 nt from human live animal serum and plasma samples 50 Preps RE-01111 Room temperature 200 Preps RE-01114
- 9. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions RNA purification series Viral RNA Isolation Kit Viral RNA Isolation Kit Rapidly isolate and purify viral RNA from samples such as plasma, serum, cell-free body fluids and cell culture supernatants 50 Preps RE-02011 Room temperature 200 Preps RE-02014 Viral DNA&RNA Isolation Kit Viral DNA&RNA Isolation Kit Quickly isolate and purify virus DNA or RNA from samples such as plasma, serum, cell-free body fluid and cell culture supernatant 50 Preps DR-01011 Room temperature 200 Preps DR-01013 Animal Total RNA Isolation Kit Animal Total RNA Isolation Kit Quickly and efficiently extract high-purity and high-quality total RNA from various animal tissues 50 Preps RE-03011 Room temperature 200 Preps RE-03014 Cell Total RNA Isolation Kit Cell Total RNA Isolation Kit Highly purified and high-quality total RNA can be obtained from various cultured cells in 11 minutes 50 Preps RE-03111 Room temperature 200 Preps RE-03113 Plant Total RNA Isolation Kit(Samples low in polysaccharides and polyphenols) Plant Total RNA Isolation Kit(Samples low in polysaccharides and polyphenols) Quickly extract high-quality total RNA from plant samples with low polysaccharide and polyphenol content 50 Preps RE-05011 Room temperature 200 Preps RE-05014 Plant Total RNA Isolation Kit Plus(Samples high in polysaccharides and polyphenols) Plant Total RNA Isolation Kit Plus(Samples high in polysaccharides and polyphenols) Quickly extract high-quality total RNA from plant samples with high content of polysaccharides and polyphenols 50 Preps RE-05021 Room temperature 200 Preps RE-05024 RNAlater(For RNA Stabilization) RNAlater(For RNA Stabilization) Fast fixation of newly taken animal sample tissues, inhibit RNase activity, protect RNA from degradation 50ml RL-01011 Room temperature RNase Eraser RNase Eraser Used in laboratories to remove RNase contamination on the solid surface, especially in RNA manipulation laboratories. 100ml RC-01011 Room temperature 100ml×15 RC-01012
- 10. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions MIX series PCR Hero ™ PCR Hero ™ Ultra-efficient PCR reaction premix system, suitable for a variety of difficult to amplify PCR templates 5ml PH-01013 -20℃ 40ml PH-01015 PCR Hero ™（With Dye) PCR Hero™（With Dye) High-efficiency PCR premix system 5ml PH-01013-B -20℃ 40ml PH-01015-B 2×High-efficiency PCR reaction premix system 2×High-efficiency PCR reaction premix systemPCR EasyTM Efficient PCR reaction premix system, suitable for various difficult to amplify PCR templates 1ml DP-20031 -20℃ 3×1.7ml DP-20032 PCR EasyTM（with dye） PCR EasyTM（with dye） Efficient PCR reaction premix system, suitable for various difficult to amplify PCR templates 3×1.7ml DP-20032-B -20℃ 50ml DP-20034-B 2×Rapid PCR reaction premix system 2×Rapid PCR reaction premix system 2× PCR Mix Fast PCR reaction premix system 1ml DP-20041 -20℃ 3×1.7ml DP-20042 Real Time PCR kit Real Time PCR EasyTM-SYBR Green I Using SYBR Green I for Real Time PCR amplification reaction can greatly improve product specificity and reaction sensitivity, and the fluorescence intensity is 3-5 times that of similar products 500 ×20μl rxns QP-01012 -20℃ 2000 ×20μl rxns QP-01014 Real Time PCR kit-Taqman Real Time PCR EasyTM- Taqman Use specific fluorescent probes for Real Time PCR amplification reaction, which can greatly improve product specificity and reaction sensitivity 500 ×20μl rxns QP-01022 -20℃ 2000 ×20μl rxns QP-01024
- 11. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions RT/RT-PCR Series Master Premix for first-strand cDNA synthesis RT EasyTM I (Master Premix for first- strand cDNA synthesis) The reverse transcription temperature of up to 65°C is conducive to the reverse transcription reaction of complex secondary structure RNA templates 25× 20μl Reactions RT-01011 -20℃ 100 × 20μl Reactions RT-01012 Master Premix for first-strand cDNA synthesis（For qPCR) RT EasyTM II (Master Premix for first- strand cDNA synthesis for Real Time PCR) Optimized reverse transcription primers have been added, only need to add RNA to perform reverse transcription, and synthesize cDNA directly for qPCR 200 × 10μl Reactions RT-01022 -20℃ 800 × 10μl Reactions RT-01023 Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase （For qPCR） RT EasyTM II(With gDNase) Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase This product is a reverse transcription system specially developed for Real Time PCR to quickly remove genomic DNA contamination. 5× gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results. 100 × 20μl Reactions RT-01032 -20℃ Super Premix for first-strand cDNA synthesis for lncRNA Lnc-RT HeroTM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA) This product is a reverse transcription system specially developed for LncRNA to quickly remove genomic DNA contamination. 5× gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results. 25 × 20μl Reactions RTL-09098 -20℃ 50 × 20μl Reactions RTL-09099 RT-PCR EasyTM I(One Step) RT-PCR EasyTMI(One Step) Realize the continuous operation from RT to PCR in the same tube, simple and fast operation, which can effectively reduce pollution during the experiment 100 rxns RT-02011 -20℃ 500 rxns RT-02012 RT-PCR EasyTM II(Two Step) RT-PCR EasyTMII(Two Step) Contains all the reagents for two-step RT-PCR, the unique RT Mix, which can easily and efficiently synthesize high-quality first-strand cDNA 100 rxns RT-02021 -20℃ 500 rxns RT-02022
- 12. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions RT/RT-PCR Series RT-qPCR EasyTM I (One Step)-SYBR Green I RT-qPCR EasyTMI (One Step)-SYBR Green I The unique system enables the effective combination of RT and Real Time PCR reactions, which can quickly complete the quantitative detection of genes. 100 rxns RT-02111 -20℃ 500 rxns RT-02112 RT-qPCR EasyTM II (One Step)-Taqman RT-qPCR EasyTMII (One Step)-Taqman Rapid and specific real-time RT-PCR quantitative detection of RNA templates 100 rxns RT-02131 -20℃ 500 rxns RT-02132 DNA Marker Series 100bp DNA Ladder 100bp DNA Ladder The 100bp DNA Ladder is composed of 13 double strands of DNA, of which the concentration of 200bp, 500bp, and 1000bp is doubled, which helps to overcome the problem of short fragments that are weak in brightness and difficult to distinguish bands. 50 Preps DL-20011 4℃ 200 Preps DL-20012 1kb DNA Ladder 1kb DNA Ladder 1Kb DNA Ladder is composed of 13 double strands of DNA, of which the doubled concentration of 1kb and 5kb is helpful to distinguish different bands 50 Preps DL-30011 4℃ 200 Preps DL-30012 Animal Direct PCR Series Animal Tissue Direct PCR Kit(65℃lysis) Animal Tissue Direct PCR Kit(65℃lysis) Directly use animal tissue lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity 200 ×20μl rxns TP-01111 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01113 Animal Tissue Direct PCR Kit(65℃lysis) -UNG Animal Tissue Direct PCR Kit(65℃lysis) -UNG Directly use animal tissue lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. Added UNG anti-pollution system to eliminate false positives 200 ×20μl rxns TP-01121 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01123 Mouse Tail SuperDirectTM PCR Kit(room temperature lysis) Mouse Tail SuperDirectTM PCR Kit(room temperature lysis) Directly use mouse tail lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity 200 ×20μl rxns TP-01311 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01313
- 13. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions Animal Direct PCR Series Mouse Tail Direct PCR Kit(65℃lysis) Mouse Tail Direct PCR Kit(65℃lysis) Directly use mouse tail lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity 200 ×20μl rxns TP-01331 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01333 Mouse Tail Direct PCR Kit(65℃lysis) -UNG Mouse Tail Direct PCR Kit(65℃lysis) -UNG The mouse tail lysate is directly used as a template for PCR amplification without separate extraction of DNA, which is fast and highly sensitive. Added UNG anti- pollution system to eliminate false positives 200 ×20μl rxns TP-01341 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01343 Zebra Fish Direct PCR Kit(65℃lysis) Zebra Fish Direct PCR Kit(65℃lysis) Directly use zebrafish lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity 200 ×20μl rxns TP-01411 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01413 Zebra Fish Direct PCR Kit(65℃lysis) -UNG Zebra Fish Direct PCR Kit(65℃lysis) -UNG Direct use of zebrafish lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. Added UNG anti-pollution system to eliminate false positives 200 ×20μl rxns TP-01421 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-01423 Blood Direct PCR Series Blood SuperDirectTM PCR Kit (EDTA) Blood SuperDirectTM PCR Kit (EDTA) Directly use EDTA anticoagulated whole blood as a template for PCR amplification, with high sensitivity and high amplification efficiency 200 ×20μl rxns TP-04111 -20℃ 2000 ×20μl rxns TP-04113 Blood SuperDirectTM PCR Kit(EDTA) -UNG Blood SuperDirectTM PCR Kit(EDTA) -UNG Directly use EDTA anticoagulated whole blood as a template for PCR amplification, with high sensitivity and high amplification efficiency. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-04121 -20℃ 2000 ×20μl rxns TP-04123 Blood SuperDirectTM PCR Kit（Heparin） Blood SuperDirectTMPCR Kit（Heparin） Directly use heparin anticoagulated whole blood as a template for PCR amplification, with high sensitivity and high amplification efficiency 200 ×20μl rxns TP-04211 -20℃ 2000 ×20μl rxns TP-04213
- 14. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions Blood Direct PCR Series Blood SuperDirectTM PCR Kit(Heparin) -UNG Blood SuperDirectTM PCR Kit(Heparin) -UNG Directly use heparin anticoagulated whole blood as a template for PCR amplification, with high sensitivity and high amplification efficiency. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-04221 -20℃ 2000 ×20μl rxns TP-04223 Plant leaf Direct PCR Series Plant Leaf Direct PCR Kit Plant Leaf Direct PCR Kit Directly use leaf or leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-02111 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-02113 Plant Leaf Direct PCR Kit -UNG Plant Leaf Direct PCR Kit -UNG Directly use leaf or leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti- pollution system has been added to prevent false positives. 200 ×20μl rxns TP-02121 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-02123 Plant Leaf Direct PCR Plus Kit（Polysaccharide polyphenol plant） Plant Leaf Direct PCR Plus Kit（Polysaccharide polyphenol plant） Directly use polysaccharide and polyphenol leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-02131 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-02133 Plant Leaf Direct PCR Plus Kit -UNG （Polysaccharide polyphenol plant） Plant Leaf Direct PCR Plus Kit -UNG （Polysaccharide polyphenol plant） Directly use polysaccharide and polyphenol leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-02141 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-02143 Plant seed Direct PCR Series Plant Seed Direct PCR Kit I Plant Seed Direct PCR Kit I Direct use of seed lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-03111 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03113 Plant Seed Direct PCR Kit I -UNG Plant Seed Direct PCR Kit I -UNG Direct use of seed lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-03121 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03123
- 15. Product Catalogues Series Product Name Descriptions Specifications Catalogue Number Storage Conditions Plant seed Direct PCR Series Plant Seed Direct PCR Kit II Plant Seed Direct PCR Kit II Direct use of seed lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-03131 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03133 Plant Seed Direct PCR Kit II -UNG Plant Seed Direct PCR Kit II -UNG Direct use of seed lysate as a template for PCR amplification, no separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-03141 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03143 Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant) Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant) Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-03151 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03153 Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-03161 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03163 Plant Seed Direct PCR Plus Kit II Plant Seed Direct PCR Plus Kit II Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. 200 ×20μl rxns TP-03171 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03173 Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. 200 ×20μl rxns TP-03181 Part I 4℃，Part II -20℃ 2000 ×20μl rxns TP-03183 Direct RT-qPCR Series Cell Direct RT-qPCR Kit–SYBR Green I QuickEasyTM Cell Direct RT-qPCR Kit–SYBR Green I Cells are lysed directly to release RNA for RT-qPCR; the high tolerance system makes it unnecessary to purify RNA and directly use cell lysates as RNA templates for RT reactions. Fast and convenient; high sensitivity, strong specificity, and good stability. 200T DRT-01011 Part I 4℃，Part II -20℃ 1000T DRT-01012
- 16. Comparison between Foregene Core Technology and Traditional Technology
- 17. Comparison between Foregene Core Technology and Traditional Technology
- 18. RNA isolation kit comparison Toxic reagents such as phenol and chloroform Complex operation and long extraction time Use DNase to remove DNA contamination Do not add any toxic reagents Easy to operate Whole process only 11min Do not add any enzymes, use DNA cleaning column 01 02 03 01 02 03 Traditional TRIzol Method Foregene RNA isolation method
- 19. Quality Control
- 20. Future • A new GMP factory will come to use in Oct-2021, life science reagents will be produced automatically. • More IVD products will be R&D and produced based on Direct PCR technology.
- 21. THANK YOU!