2. What is API?
Analytical Profile Index
kit for the identification of Enterobacteriaceae
plastic strip holding twenty mini-test tubes
3. How to use it?
The strip is inoculated with a saline
suspension of a pure culture (to rehydrate
the wells)
Most of the wells would be filled up to the
neck
Some will be filled up to the top with sterile
mineral oil
LDC ODC ADH H2S URE
Some wells will be filled all the way up to the
top
CIT VP GEL
4. How to use it?
Incubate at 37 for 18-24 h
Add the proper reagents to the compartments:
1 drop of Kovac's to the IND (read within 2 minutes)
1 drop of Barritt's A and B to VP (wait 10 minutes)
1 drop of FeCl3 to TDA