This study investigated the association between poverty and new HIV diagnoses in Houston/Harris County, Texas in 2000 and 2010. The results showed that in both years, the rate of new HIV diagnoses was positively associated with increased percentages living below the poverty line. However, the rate ratio between those in extreme poverty (>20% below poverty line) and those with low poverty (5% below poverty line) decreased from 2000 to 2010. Across both years and all poverty levels, males had higher diagnosis rates than females and African Americans remained the most impacted group. The highest rates also shifted to younger age groups living in extreme poverty in 2010, indicating targeted prevention efforts are needed for this underserved population.