1. The document summarizes an experiment exploring the use of cell-free synthesis to rapidly express the antigenic protein FhSAP2 from Fasciola hepatica. 2. The methods section describes producing antibodies against FhSAP2 in rabbits, performing ELISA tests to verify the protein's ability to react with F. hepatica sera, and using PCR and SDS-PAGE to analyze the expressed protein. 3. The results showed that FhSAP2 was successfully expressed and could be a target for developing an immunization against F. hepatica infection.

1. DIANNY PATRICIA CONSUEGRA MORA
Medical Student - UPB
Third semester

2. INTRODUCTION
New
technologies
Eukaryotic organisms
Proteins Fasciola Hepatica

3. Saponina
Human
Bubbles
Digestive
system
Immune system
Effects
Anti-tumor activity
Plants

4. OBJETIVE
“Explored the use of cell-free synthesis for
the rapid expression of an antigenic protein
belonging to the saposin-like protein family
of Fasciola hepatica (FhSAP2)”

5. MATERIALES Y METODOS
1. SUERO DE CONEJO + CONJUGADO DE ENZIMA
1. Anticuerpos
contra FhSAP2
2. Inmunización
3. Refuerzo
con proteína
4. Sangre
5. Producción de AC
ELISA
Vía oral
3. Sangre
2. Fasciola
Hepática
1. Sangre

6. 2. ELISA
Sustrato
Anti-Ac + E
Ac suero
Ag
Sustrato
Anti-Ac + E
Ac suero
Ag
Ac

7. 3. PCR
Solución:
ADN
+
Cebadores

8. 4. SDS-PAGE

9. RESULTADOS

11. Marcador de peso

13. DISCUSSION
AUTHOR HE SAID YES OR NOT
Caban-Hernandez K,
Espino
Both, monomeric and oligomeric forms of
FhSAP2 have been detected using monoclonal
antibodies in soluble crude extracts of F.
hepatica adult flukes
Espino AM, Morales A,
Delgado B, Rivera FM,
Figueroa O, Suarez E
A previously optimized ELISA was used
to test the protein’s capacity to react
with a pool of sera raised against F.
hepatica
Espino AM, Hillyer GV We had previously demonstrated that
FhSAP2 is a component of the F.
hepatica ES products

14. CONCLUSION
• This could be the beginning of studies to develop an immunization against
Fasciola hepatica and contribute to decrease the risk of fascioliasis that
these parasites generate in organisms
• FhSAP2 is a protein that appears in the early stage
of infection. then it could be determined to identify
Fasciola Hepatica in an organism and thus avoid greater risks
of Fascioliasis