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4. Using the technique of interrupted mating, five Hfr strains were tested for the sequence in
which they transmitted a number of different genes to an F- strain. Each Hfr strain was found to
transmit its genes in a unique sequence, as follows (only the first six genes transmitted were
scored). 1. A G B F C K 2. FCKDJE 3. D J EHA G 4. J D K C F B 5. F B GAHE A. Place all
markers in order on a single circular genetic map. B. Show the location and orientation of the F
factor in each Hfr strain. C. For each of the Hfr strains, state which donor-gene marker should be
selected in the recipients after conjugation to obtain the highest proportion of recombinants that
would be converted to become Hfrs. Strain 1 Strain 2 Strain 3 Strain 4 Strain 5

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