Beginners Guide to TikTok for Search - Rachel Pearson - We are Tilt __ Bright...
Mnemonics
1.
2.
3. Mnemonics
C DNA definition:
• cOOL sWAg MOVES
Collection of only specific genes.
Screening:
• pets house
probes for hybridization
4. Types of screening methods
Bride cop...
B – Blue White screening.
R – Replica plating.
I – Immunological screening.
D – Differential screening.
E – Expression screening.
C – Colony hybridization.
O – Oligonucleotide probe.
P – Plaque hybridization.
5. Colony Hybridization
Definition:
• Coach having bat
Colony hybridization is a Blot Analysis
Technique.
Master Plate:
• Ibaco sam
I – Inoculation
B – Bacterial
C – Cells
SAM - Solid Agar Medium
6. Materials
• name first
N – Nitrocellulose
M – Membrane
F - Filter
• Shades are rapt want glows for costly
SDS – Sodium Dodecyl Sulphate
A – Alkali
R – Radioactive
P - Probe
W – Washing
A - Autoradiography
G – Get
SC – Specific Copies.
7. Plaque hybridization
Definition:
• Morning beats rock paradise
In molecular biology ,for identify
Recombinant Phages.
Steps:
• School
Similar, to colony hybridization
label probe for gene of study.
• Adp
A – Autoradiography
D - Detect
P - Position
8. • be at smooth wind daily
A – Advantages
O –Over
C – Colony hybridization
S – Smaller
W – Well
D – Defined
F – Filter.
10. • paradise calls day- to- day foot prints
P – Production
S – Substracted DNA
L – Libraries
D – Differential
D – Display
FP – Foot Printing(RNA)
11. IMMUNOLOGICAL SCREENING
• A PACE SANTIFY
A – Adding
P – Primary
A – Antibody
S – Secondary
A – Antibody.
• App hope
A – Alkaline
P – Phosphate
H – Horseradish
P- Peroxidase
12. Blue white screening
• Puff re
P –Plasmid DNA
F – Foreign DNA
RE – Restriction Enzyme
• Rock party
R – Recombinant
P –Plasmid
A R- amp resistance
• Bc wc
BC – Blue colonies (not contain foreign DNA)
WC – White colonies(having foreign DNA)
13. REPLICA- PLATING
• MOON TRAVEL
M – Microbiological
T – Technique
• Sangam
S – Selective
G – Growth
M – Media
• Vcd= velveteen covered disk
• Ns= negative selection
• A part= amp-resistance ,phenotype includes Autotrophy
and Antibiotic Resistance.
14. EXPRESSION SCREENING
• A ETHUSIASTIC LOOP
A – Antibody
E – Expression
L – Library
• Pride race
P – Plaque lift
I – Immersed
D- Detected
R – Repeat cycles.