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Hawthorn	
  Pollinator	
  Examination	
  
Jessica	
  Snow	
  
Biol	
  320	
  Sec	
  001	
  
08	
  VI	
  2015	
  
All	
  photos	
  by	
  Jessica	
  Snow	
  
Data	
  Collected	
  by	
  Anna	
  Wan,	
  Sam	
  Walker,	
  and	
  Jessica	
  Snow	
  
	
  
	
  
	
  
2	
  
Table	
  of	
  Contents:	
  
	
  
	
  
	
  
	
  
	
  
	
  
Introductions_______________________________________________________________________________________3	
  
Methods_____________________________________________________________________________________________3	
  
Results_______________________________________________________________________________________________4	
  
Discussion___________________________________________________________________________________________6	
  
Key	
  for	
  Basic	
  Identification________________________________________________________________________7	
  
Conclusion_________________________________________________________________________________________10	
  
References_________________________________________________________________________________________11	
  
Appendix	
  A________________________________________________________________________________________12	
  
	
  
	
   	
  
3	
  
Introduction	
  
Pollination,	
  primarily	
  by	
  insects,	
  is	
  one	
  of	
  the	
  most	
  important	
  ecological	
  services	
  
provided	
  by	
  a	
  group	
  of	
  organisms.	
  Pollination	
  is	
  key	
  to	
  sexual	
  reproduction	
  in	
  many	
  
flowering	
  plants,	
  and	
  allows	
  for	
  the	
  continued	
  introduction	
  of	
  variation	
  by	
  genetic	
  
recombination	
  (Clancy).	
  This	
  genetic	
  variation	
  is	
  vital	
  to	
  stable	
  communities,	
  and	
  with	
  
variation	
  comes	
  the	
  ability	
  to	
  survive	
  environmental	
  changes.	
  In	
  addition,	
  pollination	
  is	
  
needed	
  for	
  the	
  production	
  of	
  35%	
  of	
  the	
  crops	
  used	
  for	
  food	
  worldwide	
  (Insects	
  &	
  
Pollinators).	
  With	
  this	
  in	
  mine,	
  it	
  becomes	
  apparent	
  how	
  crucial	
  it	
  is	
  to	
  support	
  native	
  
pollinator	
  species.	
  Genus	
  Crataegus,	
  or	
  commonly	
  called,	
  Hawthorn	
  trees	
  have	
  multiple	
  
species	
  native	
  to	
  Michigan	
  (Michigan	
  Flora	
  Online).	
  Many	
  of	
  these	
  trees	
  are	
  known	
  to	
  help	
  
support	
  other	
  species	
  by	
  providing	
  food	
  and	
  shelter.	
  In	
  our	
  study,	
  we	
  surveyed	
  the	
  
individuals	
  visiting	
  Hawthorn	
  trees	
  while	
  in	
  bloom.	
  This	
  survey	
  shows	
  Hawthorn	
  tree’s	
  
ability	
  to	
  support	
  local	
  pollinator	
  species	
  by	
  providing	
  them	
  with	
  a	
  seasonal	
  food	
  source.	
  	
  
	
  
Methods	
  
	
  
	
   To	
  learn	
  about	
  the	
  diversity	
  of	
  
pollinators	
  that	
  visit	
  the	
  Hawthorn	
  trees	
  in	
  
Jensen’s	
  meadow,	
  collections	
  were	
  made	
  on	
  
four	
  days	
  from	
  two	
  sites.	
  Both	
  sites	
  of	
  
collection	
  were	
  adult	
  Hawthorn	
  trees	
  found	
  
in	
  Jensen’s	
  meadow	
  on	
  the	
  University	
  of	
  
Dearborn	
  Campus	
  Natural	
  Area	
  (figure	
  1).	
  
Collection	
  periods	
  were	
  conducted	
  in	
  late	
  
morning	
  to	
  achieve	
  best	
  collection	
  condition.	
  
Goal	
  conditions	
  were	
  full	
  sun,	
  calm,	
  and	
  
warm.	
  Due	
  to	
  natural	
  weather	
  variation,	
  not	
  
all	
  collections	
  could	
  be	
  made	
  in	
  these	
  prime	
  conditions	
  (table	
  1).	
  On	
  May	
  22,	
  collection	
  
techniques	
  were	
  practiced	
  and	
  no	
  specimens	
  were	
  analyzed.	
  Specimens	
  were	
  collected	
  
using	
  fine	
  mesh	
  nets,	
  transferred	
  into	
  plastic	
  transfer	
  containers	
  and	
  stored	
  in	
  a	
  shaded	
  
region	
  until	
  the	
  collection	
  was	
  complete.	
  During	
  collection,	
  repeat	
  specimens	
  were	
  not	
  
collected	
  if	
  possible.	
  Collection	
  focused	
  on	
  examining	
  the	
  diversity,	
  not	
  quantity,	
  of	
  
	
  
Figure	
  1:	
  Map	
  of	
  Collection	
  Area	
  
4	
  
pollinators	
  visiting	
  the	
  Hawthorn	
  trees.	
  	
  After	
  collection	
  was	
  competed,	
  specimens	
  were	
  
refrigerated	
  for	
  20-­‐35m	
  and	
  then	
  transferred	
  to	
  individual	
  vials	
  for	
  observation.	
  These	
  
vials	
  were	
  kept	
  refrigerated	
  or	
  in	
  ice	
  water	
  when	
  not	
  being	
  analyzed	
  to	
  inhibit	
  movement	
  
and	
  stress	
  of	
  specimens	
  collected.	
  They	
  were	
  then	
  observed	
  under	
  a	
  dissecting	
  microscope	
  
and	
  photographed.	
  A	
  record	
  was	
  taken	
  of	
  each	
  identified	
  pollinator.	
  To	
  help	
  in	
  
identification,	
  Kaufman’s	
  Field	
  Guide	
  of	
  Insects	
  and	
  BugGuide.net	
  were	
  used.	
  Collection	
  
ended	
  with	
  the	
  end	
  of	
  the	
  blooming	
  period	
  for	
  the	
  Hawthorn	
  trees,	
  which	
  bloomed	
  for	
  just	
  
over	
  one	
  week.	
  Organisms	
  were	
  identified	
  to	
  their	
  family,	
  a	
  few	
  were	
  identified	
  to	
  genus,	
  
some	
  were	
  tentatively	
  identified	
  to	
  genus,	
  and	
  two	
  were	
  identified	
  to	
  species.	
  In	
  analysis	
  of	
  
diversity,	
  different	
  specimens	
  in	
  the	
  same	
  family	
  were	
  only	
  considered	
  different	
  if	
  they	
  
were	
  morphologically	
  unique.	
  In	
  this	
  case,	
  they	
  were	
  given	
  a	
  number	
  after	
  their	
  name	
  to	
  
denote	
  this	
  difference	
  (table	
  2,	
  appendix	
  A).	
  
	
  
Results	
  
	
   From	
  four	
  days	
  of	
  collection,	
  25	
  unique	
  organisms	
  were	
  identified	
  to	
  be	
  visiting	
  the	
  
Hawthorn	
  trees.	
  Of	
  these	
  species,	
  not	
  all	
  are	
  known	
  to	
  collect	
  pollen	
  or	
  nectar,	
  instead	
  
using	
  the	
  Hawthorn	
  for	
  other	
  purposes	
  such	
  as	
  feeding	
  on	
  plants	
  and	
  insects.	
  These	
  will	
  be	
  
considered	
  secondary	
  pollinators	
  and	
  are	
  denoted	
  in	
  table	
  2	
  with	
  an	
  asterisk.	
  Kaufman’s	
  
Filed	
  Guide	
  to	
  insects	
  was	
  used	
  to	
  make	
  this	
  determination	
  (Kaufman).	
  To	
  see	
  further	
  
details	
  about	
  behaviors	
  that	
  qualified	
  organisms	
  as	
  primary	
  or	
  secondary	
  pollinators,	
  refer	
  
to	
  Appendix	
  A.	
  In	
  total,	
  17	
  commonly	
  considered	
  pollinators,	
  which	
  we	
  will	
  call	
  primary	
  
pollinators,	
  were	
  identified.	
  8	
  secondary	
  pollinators	
  were	
  collected.	
  Many	
  secondary	
  
pollinators	
  accidentally	
  pollinate	
  flowers	
  while	
  performing	
  their	
  own	
  feeding	
  behavior.	
  
Day	
   Test	
   A	
   B	
   C	
   D	
  
Date	
   22	
  V	
  2015	
   23	
  V	
  2015	
   27	
  V	
  2015	
   29	
  V	
  2015	
   01	
  V1	
  2015	
  
Time	
  (EST)	
   1230-­‐0130	
   1046-­‐1130	
   0905-­‐1005	
   0920-­‐1020	
   1220-­‐1250	
  
Hawthorn	
  sampled	
  	
   A	
   A	
   A	
   A	
   B	
  
Temperature	
  (°C)	
   N/A	
   25.9	
   26.0	
   25.6	
   13	
  
Humidity	
  (%)	
   N/A	
   32	
   61	
   78	
   58	
  
Sky	
  cover	
   N/A	
   Clear	
   Clear	
   Full	
  cloud	
   Full	
  cloud	
  
Wind	
   N/A	
   Calm	
   Calm	
   Calm	
   1.5mph	
  
Analysis	
  Time	
   N/A	
   1130-­‐0300	
   1205-­‐0305	
   1210-­‐0310	
   1250-­‐0150	
  
Bloom	
  Stage	
   Early	
  	
   Middle	
   Middle	
   Fading	
   Late	
  
Table	
  1:	
  Collection	
  Information	
  
5	
  
This	
  fact	
  	
  was	
  determined	
  from	
  the	
  visible	
  presence	
  of	
  pollen	
  grains	
  on	
  many	
  of	
  these	
  
individuals.	
  Day	
  B,	
  May	
  27th	
  had	
  the	
  best	
  collecting	
  conditions	
  and	
  was	
  the	
  time	
  of	
  fullest	
  
flower	
  blooms.	
  This	
  day	
  had	
  sunshine	
  and	
  high	
  temperatures,	
  which	
  seemed	
  to	
  encourage	
  
pollination	
  behavior.	
  Day	
  B	
  was	
  a	
  diverse	
  collection	
  day,	
  and	
  the	
  majority	
  of	
  species	
  
collected	
  were	
  known	
  pollinators.	
  On	
  day	
  B,	
  10	
  different	
  types	
  of	
  pollinators	
  were	
  collected	
  
and	
  90%	
  were	
  primary	
  pollinators	
  (table	
  2).	
  On	
  day	
  C,	
  12	
  types	
  of	
  pollinators	
  were	
  
collected,	
  but	
  only	
  50%	
  were	
  primary	
  pollinators.	
  Day	
  A	
  was	
  much	
  less	
  diverse	
  with	
  only	
  6	
  
types	
  collected,	
  80%	
  of	
  them	
  being	
  primary	
  pollinators.	
  The	
  day	
  of	
  lowest	
  diversity	
  was	
  
day	
  D,	
  with	
  only	
  two	
  individuals	
  collected,	
  both	
  being	
  primary	
  pollinators.	
  This	
  could	
  be	
  
due	
  both	
  to	
  cold	
  cloudy	
  weather	
  and	
  the	
  end	
  of	
  bloom	
  conditions.	
  Mining	
  bees	
  were	
  
	
   23	
  V	
  2015	
   27	
  V	
  2015	
   29	
  V	
  2015	
   01	
  V1	
  2015	
  
Small	
  Carpenter	
  Bee	
   II	
   	
   	
   	
  
Mining	
  Bee	
   IIII	
   III	
   I	
   	
  
Asian	
  Multicolored	
  Lady	
  Beetle*	
   I	
   	
   	
   	
  
Spotted	
  Lady	
  Beetle	
   I	
   	
   	
   	
  
Metallic	
  Sweat	
  Bee	
   II	
   	
   	
   	
  
Flesh	
  Fly	
   I	
   	
   I	
   	
  
Muscid	
  Fly*	
   	
   I	
   	
   	
  
Bee	
  Fly	
   	
   I	
   	
   	
  
Bumble	
  Flower	
  Beetle	
   	
   I	
   	
   	
  
Large	
  Carpenter	
  Bee	
   	
   I	
   	
   	
  
Red	
  Admiral	
  Butterfly	
   	
   I	
   I	
   	
  
Sweat	
  Bee	
   	
   I	
   II	
   	
  
Honey	
  Bee	
   	
   I	
   	
   	
  
Flower	
  Fly	
  1	
   	
   I	
   	
   	
  
Flower	
  Fly	
  2	
   	
   I	
   	
   	
  
Plant	
  Bug*	
   	
   	
   I	
   	
  
Spread-­‐winged	
  Damselfly*	
   	
   	
   II	
   	
  
Rove	
  Beetle	
  1*	
   	
   	
   I	
   	
  
Rove	
  Beetle	
  2*	
   	
   	
   I	
   	
  
Assassin	
  Bug*	
   	
   	
   I	
   	
  
Yellowjacket	
   	
   	
   I	
   	
  
Bumble	
  Flower	
  Beetle	
  1	
   	
   	
   I	
   	
  
Thread-­‐waisted	
  Wasp*	
   	
   	
   I	
   	
  
Ichneumon	
  Wasp	
   	
   	
   	
   I	
  
Syrphid	
  Fly	
  	
   	
   	
   	
   I	
  
Table	
  2:	
  Collection	
  Results	
  
*Secondary	
  Pollinator	
  
6	
  
collected	
  on	
  three	
  of	
  the	
  four	
  collection	
  days,	
  making	
  them	
  a	
  common	
  pollinator	
  of	
  
Hawthorns.	
  Flesh	
  flies,	
  Red	
  Admiral	
  butterflies,	
  and	
  Sweat	
  bees	
  were	
  all	
  collected	
  on	
  two	
  
occasions,	
  meaning	
  that	
  they	
  may	
  be	
  somewhat	
  common	
  pollinators	
  for	
  Hawthorn	
  trees.	
  
	
  
Discussion	
  
	
   The	
  taxa	
  richness	
  observed	
  at	
  the	
  Hawthorn	
  trees	
  during	
  the	
  4	
  days	
  of	
  collection	
  
show	
  that	
  they	
  are	
  host	
  to	
  many	
  different	
  pollinators.	
  	
  It	
  can	
  be	
  surmised	
  that	
  Hawthorn	
  
trees	
  are	
  capable	
  of	
  helping	
  to	
  support	
  a	
  largely	
  diverse	
  population	
  of	
  pollinators.	
  This	
  
allows	
  for	
  the	
  opportunity	
  to	
  introduce	
  Hawthorn	
  trees	
  to	
  an	
  area	
  where	
  the	
  pollinators	
  
found	
  in	
  this	
  study	
  would	
  be	
  a	
  positive	
  addition.	
  In	
  habitat	
  management,	
  this	
  data	
  can	
  be	
  
used	
  as	
  a	
  guide	
  to	
  look	
  at	
  benefits	
  and	
  drawbacks	
  of	
  adding	
  Hawthorn	
  trees	
  into	
  an	
  
environment.	
  
	
   Methods	
  of	
  identification	
  were	
  learned	
  by	
  trial	
  and	
  error	
  during	
  the	
  4	
  days	
  of	
  
collection.	
  	
  The	
  Key	
  below	
  was	
  created	
  as	
  a	
  summary	
  of	
  the	
  best	
  techniques	
  acquired.	
  In	
  
our	
  identification,	
  the	
  first	
  necessary	
  step	
  was	
  to	
  isolate	
  the	
  invertebrates	
  into	
  broad	
  
categories.	
  After	
  that	
  was	
  determined	
  more	
  specific	
  classifications	
  were	
  made.	
  	
  
	
  
Key	
  For	
  Basic	
  Identification	
  
1. Can	
  haltêres	
  (small	
  knob	
  structures)	
  be	
  identified	
  behind	
  the	
  front	
  wing?	
  
a. If	
  yes,	
  you	
  have	
  a	
  fly.	
  Flies	
  only	
  have	
  one	
  set	
  of	
  wings	
  which	
  often	
  fold	
  in.	
  
	
  
Example	
  of	
  a	
  fly	
  (left)	
  and	
  close-­‐up	
  of	
  haltêre	
  (right)	
  
b. If	
  no,	
  continue	
  to	
  question	
  two.	
  
7	
  
	
  
2. Can	
  elytra	
  (hardened	
  wing	
  cover)	
  be	
  observed	
  in	
  rest,	
  often	
  concealing	
  wings?	
  
a. If	
  yes,	
  you	
  have	
  a	
  beetle.	
  	
  
	
  
Examples	
  of	
  two	
  types	
  of	
  beetles	
  with	
  hard	
  wing-­‐covers	
  (above)	
  
b. If	
  no,	
  continue	
  on	
  to	
  question	
  three.	
  
3. Are	
  there	
  broad	
  wings,	
  more	
  than	
  3	
  times	
  the	
  size	
  of	
  body?	
  
a. If	
  yes,	
  you	
  have	
  a	
  butterfly,	
  or	
  moth.	
  
	
  
Examples	
  of	
  a	
  Red	
  Admiral	
  Butterfly	
  (above)	
  
b. If	
  no,	
  continue	
  on	
  to	
  question	
  four.	
  
	
  
	
  
	
  
8	
  
	
  
	
  
4. Is	
  the	
  abdomen	
  very	
  narrow,	
  2	
  pairs	
  of	
  wings	
  long,	
  and	
  body	
  often	
  blue	
  or	
  green?	
  
a. If	
  yes,	
  you	
  have	
  a	
  damselfly	
  or	
  pondhawk	
  
	
  
Example	
  of	
  Spread-­‐winged	
  Damselfly	
  (above)	
  
b. If	
  no,	
  continue	
  on	
  to	
  question	
  five.	
  
5. Are	
  legs	
  much	
  longer	
  than	
  body,	
  highly	
  jointed,	
  or	
  body	
  with	
  highly	
  angular	
  shape?	
  
a. If	
  yes,	
  you	
  may	
  have	
  a	
  plant	
  bug,	
  assassin	
  bug	
  or	
  other	
  secondary	
  pollinator.	
  
	
  
Example	
  of	
  plant	
  bug	
  (left)	
  and	
  assassin	
  bug	
  (right)	
  
b. If	
  no,	
  continue	
  on	
  to	
  question	
  six.	
  
	
  
	
  
9	
  
	
  
6. Is	
  connection	
  between	
  thorax	
  and	
  abdomen	
  very	
  narrow	
  or	
  hourglass	
  shaped?	
  
a. If	
  yes,	
  you	
  may	
  have	
  a	
  wasp	
  or	
  similar	
  family	
  
	
  
Example	
  of	
  Thread-­‐Waisted	
  (Left)	
  and	
  Ichneumon	
  Wasp	
  (right)	
  
b. If	
  no,	
  you	
  may	
  have	
  a	
  bee	
  if	
  it	
  fits	
  these	
  criterion:	
  hairy	
  or	
  not,	
  shiny	
  or	
  not,	
  
compound	
  eyes	
  on	
  sides	
  of	
  head,	
  antennae	
  usually	
  moderately	
  long,	
  legs	
  
thicker,	
  joined	
  and	
  shorter	
  than	
  body	
  length.	
  Holds	
  wings	
  to	
  body	
  when	
  at	
  
rest.	
  Yellowjackets	
  have	
  yellow	
  and	
  black	
  distinct	
  patterning	
  on	
  abdomen.	
  
	
  
Example	
  of	
  sweat	
  bee	
  (left)	
  and	
  yellowjacket	
  (right)	
  
This	
  experience	
  showed	
  that	
  insect	
  identification	
  is	
  not	
  black	
  and	
  white,	
  which	
  is	
  
why	
  identification	
  keys	
  are	
  tricky	
  to	
  make.	
  The	
  purpose	
  of	
  the	
  above	
  dichotomous	
  key	
  is	
  to	
  
help	
  identify	
  common	
  pollinators	
  found	
  at	
  Hawthorn	
  trees,	
  and	
  was	
  the	
  approach	
  
10	
  
developed	
  during	
  identification.	
  The	
  key	
  can	
  be	
  used	
  as	
  a	
  good	
  way	
  to	
  start	
  figuring	
  out	
  
what	
  type	
  of	
  arthropod	
  you	
  are	
  dealing	
  with.	
  The	
  rules	
  above	
  will	
  have	
  exceptions	
  and	
  
won’t	
  be	
  accurate	
  in	
  all	
  cases.	
  It	
  is	
  hoped	
  that	
  with	
  resources	
  for	
  easier	
  identification,	
  
important	
  pollinators	
  will	
  be	
  recognized	
  and	
  appreciated	
  for	
  their	
  high	
  ecological	
  value	
  as	
  
well	
  as	
  for	
  intrinsic	
  value.	
  
This	
  study	
  was	
  completed	
  in	
  hopes	
  that	
  further	
  research	
  will	
  be	
  conducted	
  on	
  the	
  
same	
  and	
  other	
  local	
  species	
  that	
  support	
  diverse	
  and	
  beneficial	
  pollinator	
  populations.	
  
This	
  study	
  should	
  be	
  followed	
  up	
  with	
  the	
  following	
  considerations.	
  The	
  collection	
  may	
  
have	
  been	
  biased	
  to	
  attractive,	
  moving,	
  loud	
  pollinators	
  and	
  therefore	
  a	
  representative	
  
sample	
  may	
  not	
  have	
  been	
  collected.	
  Repeat	
  observations	
  need	
  to	
  be	
  conducted	
  to	
  see	
  if	
  the	
  
recorded	
  pollinator	
  population	
  is	
  consistent	
  on	
  a	
  yearly	
  basis.	
  Further	
  analysis	
  of	
  identified	
  
pollinators	
  should	
  occur	
  to	
  further	
  classify	
  and	
  understand	
  the	
  diversity	
  of	
  pollinators	
  
supported	
  by	
  Hawthorn	
  trees.	
  	
  
	
  
Conclusion	
  
The	
  survey	
  of	
  pollinators	
  at	
  Hawthorn	
  trees	
  shows	
  signs	
  of	
  high	
  diversity,	
  
suggesting	
  that	
  Hawthorns	
  are	
  important	
  members	
  of	
  a	
  community	
  that	
  is	
  able	
  to	
  support	
  
pollinators.	
  By	
  supporting	
  many	
  pollinating	
  organisms,	
  addition	
  of	
  Hawthorn	
  trees	
  into	
  
meadow	
  environments	
  is	
  recommended	
  when	
  other	
  flower	
  plants	
  exist	
  to	
  support	
  the	
  
pollinator	
  population	
  for	
  the	
  355	
  days	
  of	
  the	
  year	
  when	
  Hawthorn’s	
  are	
  not	
  in	
  bloom.	
  In	
  
this	
  way,	
  Hawthorns	
  can	
  contribute	
  to	
  a	
  healthy	
  community	
  and	
  provide	
  a	
  stable	
  food	
  
source	
  for	
  these	
  pollinators.	
  This	
  study	
  can	
  help	
  initiate	
  further	
  research	
  into	
  local	
  
pollinator	
  populations	
  and	
  the	
  plant	
  species	
  that	
  support	
  them,	
  helping	
  us	
  understand	
  the	
  
biotic	
  relationships	
  that	
  support	
  the	
  communities	
  and	
  populations	
  we	
  live	
  in.	
  This	
  will	
  
allow	
  us	
  to	
  make	
  educated	
  and	
  responsible	
  decisions	
  about	
  habitat	
  management.	
  
	
   	
  
11	
  
References	
  
	
  
Kaufman,	
  Kenn,	
  and	
  Eric	
  R.	
  Eaton.	
  Kaufman	
  Field	
  Guide	
  to	
  Insects	
  of	
  North	
  America.	
  New	
  
York,	
  NY:	
  Houghton	
  Mifflin,	
  2007.	
  Print.	
  
BugGuide.	
  Iowa	
  State	
  University,	
  Department	
  of	
  Entomology,	
  2015.	
  Web.	
  08	
  June	
  2015.	
  
<http://bugguide.net/>.	
  
Michigan	
  Flora	
  Online.	
  A.	
  A.	
  Reznicek,	
  E.	
  G.	
  Voss,	
  &	
  B.	
  S.	
  Walters.	
  February	
  2011.	
  University	
  
of	
  Michigan.	
  Web.	
  June	
  8,	
  2015.	
  http://michiganflora.net/genus.aspx?id=Crataegus.	
  
Clancy,	
  S.	
  (2008)	
  Genetic	
  recombination.	
  Nature	
  Education	
  1(1):40	
  <	
  
http://www.nature.com/scitable/topicpage/genetic-­‐recombination-­‐514>.	
  
"Insects	
  &	
  Pollinators."	
  Natural	
  Resources	
  Conservation	
  Service.	
  United	
  States	
  Department	
  
of	
  Agriculture,	
  2015.	
  Web.	
  08	
  June	
  2015.	
  <http://www.nrcs.usda.gov/wps/portal/	
  
nrcs/main/national/plantsanimals/pollinate/>.	
  
	
   	
  
12	
  
Appendix	
  A:	
  
	
  Behavior	
  Information	
  used	
  to	
  Determine	
  Primary	
  vs.	
  Secondary	
  Pollinators	
  
	
  
	
   Diet	
  or	
  Behavioral	
  Trail	
  
Small	
  Carpenter	
  Bee	
   Thistles	
  and	
  other	
  flowers	
  
Mining	
  Bee	
   Pollen	
  and	
  nectar	
  from	
  specific	
  flowers	
  
Asian	
  Multicolored	
  Lady	
  Beetle*	
   Aphids	
  
Spotted	
  Lady	
  Beetle	
   Eats	
  aphids	
  and	
  pollen	
  
Metallic	
  Sweat	
  Bee	
   Known	
  to	
  pollinate	
  flowers	
  
Flesh	
  Fly	
   Adults	
  eat	
  flower	
  nectar	
  
Muscid	
  Fly*	
   Adults	
  feed	
  on	
  living	
  plant	
  or	
  animal	
  tissue	
  
Bee	
  Fly	
   Eats	
  nectar	
  of	
  lilac,	
  plum	
  and	
  other	
  flowers	
  
Bumble	
  Flower	
  Beetle	
   Feed	
  on	
  pollen,	
  nectar,	
  fruit	
  and	
  plant	
  juices	
  
Large	
  Carpenter	
  Bee	
   Pollinate	
  apples,	
  pears	
  and	
  other	
  fruit	
  
Red	
  Admiral	
  Butterfly	
   Adults	
  eat	
  fruit,	
  sap,	
  bird	
  droppings	
  and	
  nectar	
  
Sweat	
  Bee	
   Known	
  to	
  pollinate	
  flowers	
  
Honey	
  Bee	
   Pollinate	
  flowers	
  
Flower	
  Fly	
  1	
   Feed	
  on	
  many	
  flower	
  parts	
  
Flower	
  Fly	
  2	
   Feed	
  on	
  many	
  flower	
  parts	
  
Plant	
  Bug*	
   Feed	
  on	
  plants,	
  some	
  are	
  predatory	
  
Spread-­‐winged	
  Damselfly*	
   Feed	
  on	
  insects	
  
Rove	
  Beetle	
  1*	
   Mostly	
  insect	
  eaters	
  
Rove	
  Beetle	
  2*	
   Mostly	
  insect	
  eaters	
  
Assassin	
  Bug*	
   Eat	
  insects	
  
Yellowjacket	
   Adults	
  collect	
  flower	
  nectar	
  
Bumble	
  Flower	
  Beetle	
  2	
   Feed	
  on	
  pollen,	
  nectar,	
  fruit	
  and	
  plant	
  juices	
  
Thread-­‐waisted	
  Wasp*	
   Eat	
  flies	
  
Ichneumon	
  Wasp	
   Some	
  are	
  parasites,	
  others	
  visit	
  flowers	
  
Syrphid	
  Fly	
  	
   Feed	
  on	
  flower	
  parts	
  
*Gray	
  and	
  asterisks	
  means	
  the	
  organism	
  has	
  been	
  determined	
  to	
  be	
  a	
  secondary	
  pollinator.	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
   	
  
13	
  
Appendix	
  B:	
  	
  
Species	
  List	
  
	
  
Methods	
  of	
  Collection	
  
1. Collected	
  specimens	
  only	
  that	
  landed	
  on	
  Hawthorn	
  tree	
  near	
  flower	
  or	
  hover	
  nearby	
  
2. Moths	
  and	
  caterpillars	
  released	
  
3. Smallest	
  insects	
  could	
  not	
  be	
  captured	
  using	
  our	
  methods	
  
4. Repeat	
  specimen	
  collections	
  for	
  a	
  day	
  were	
  avoided	
  when	
  detected	
  
5. Stored	
  in	
  plastic	
  containers	
  in	
  shade	
  during	
  collection	
  
6. Collections	
  limited	
  to	
  one	
  hour	
  	
  
7. Specimen	
  were	
  stored	
  in	
  refrigerator	
  20min	
  or	
  more	
  before	
  analysis	
  
8. Identified	
  used	
  dissecting	
  microscopes	
  and	
  a	
  camera	
  
9. Identified	
  specimens	
  were	
  released	
  S.	
  of	
  EIC	
  
	
  
	
  
	
  
A1	
  Small	
  Carpenter	
  Bee	
  
Family:	
  Apidae	
  
Genus:	
  Ceratina	
  
	
  
ID:	
  7cm	
  long,	
  
ID:	
  head	
  and	
  thorax	
  iridescent	
  dark	
  green,	
  almost	
  black	
  
ID:	
  very	
  few	
  hairs	
  on	
  head,	
  abdomen	
  and	
  thorax	
  
ID:	
  Distinguished	
  from	
  small	
  sweat	
  bees	
  because	
  less	
  hair	
  and	
  lobed	
  back	
  wing	
  
Pollen	
  hairs	
  on	
  3rd	
  pair	
  of	
  legs	
  only	
  
Slender	
  body	
  
Small	
  mouthparts	
  
Long	
  slender	
  wings	
  
(Kaufman	
  p.348)	
  
	
  
	
  
	
  
14	
  
A2	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  
ID:	
  very	
  long	
  hairs	
  on	
  3rd	
  pair	
  of	
  legs	
  
(Kaufman	
  p.340)	
  
	
  
A3	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  	
  
Long,	
  slender	
  wings	
  
Yellow	
  hairs	
  on	
  full	
  body	
  and	
  legs	
  
10mm	
  long	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
15	
  
A4	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  	
  
(Kaufman	
  p.340)	
  
	
  
A5	
  Small	
  Carpenter	
  Bee	
  
Family:	
  Apidae	
  
Genus:	
  Ceratina	
  
	
  
ID:	
  slender	
  full	
  iridescent	
  green	
  body	
  
ID:	
  very	
  few	
  hairs	
  on	
  head,	
  abdomen	
  and	
  thorax	
  
Pollen	
  in	
  long	
  hairs	
  on	
  3rd	
  leg	
  pair	
  
8.5mm	
  long	
  
(Kaufman	
  p.348)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
16	
  
A6	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  	
  
(Kaufman	
  p.340)	
  
	
  
A7	
  Asian	
  Multicolored	
  Lady	
  Beetle	
  
Family:	
  Coccinellidae	
  
Species:	
  Harmonia	
  axyridis	
  
	
  
ID:	
  Black	
  M-­‐shaped	
  pattern	
  on	
  white	
  pronotum	
  
ID:	
  Elytra	
  orange,	
  shiny	
  with	
  black	
  spots	
  
ID:	
  a	
  beetle	
  due	
  to	
  hardened	
  elytra	
  
(Kaufman	
  p.156)	
  
	
  
	
  
	
  
	
  
	
  
	
  
17	
  
A8	
  Spotted	
  Lady	
  Beetle	
  
Family:	
  Coccinellidae	
  	
  
Species:	
  coleomegilla	
  maculata	
  
	
  
ID:	
  Elytra	
  orange	
  with	
  12	
  large	
  black	
  spots,	
  pronotum	
  orange	
  with	
  two	
  black	
  spots	
  
ID:	
  flattened	
  back,	
  long	
  abdomen,	
  black	
  legs	
  
ID:	
  a	
  beetle	
  due	
  to	
  hardened	
  elytra	
  
(Kaufman	
  p.154)	
  
	
  
A9	
  Metallic	
  Sweat	
  Bee	
  
Family:	
  Halictidae	
  
Genus:	
  probably	
  Augochlora	
  or	
  Augochlorella	
  
	
  
ID:	
  Entire	
  body	
  metallic	
  green	
  and	
  hairy	
  
Fine	
  yellow	
  hairs	
  on	
  all	
  legs	
  
Abdomen	
  slender,	
  curved	
  down	
  
9.5mm	
  long	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
A10	
  Released,	
  Not	
  a	
  Pollinator	
  
	
  
18	
  
A11	
  Flesh	
  Fly	
  
Family:	
  Sarcophagidae	
  
	
  
ID:	
  one	
  pair	
  wings,	
  white	
  large	
  haltêres	
  
ID:	
  short	
  antennae	
  
Red-­‐brown	
  eyes,	
  thorax	
  with	
  black	
  and	
  silver	
  vertical	
  stripes,	
  hairy	
  
Feet	
  with	
  two	
  pads,	
  wings	
  lay	
  flat	
  
(Kaufman	
  p.306)	
  
	
  
A12	
  Metallic	
  Sweat	
  Bee	
  
Family:	
  Halictidae	
  
	
  
ID:	
  Entire	
  body	
  metallic	
  green	
  and	
  hairy,	
  two	
  sets	
  of	
  wings	
  
Abdomen	
  eaten	
  by	
  other	
  insect	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
	
  
	
  
19	
  
B1	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  
(Kaufman	
  p.340)	
  
	
  
B2	
  Muscid	
  Fly	
  
Family:	
  Muscidae	
  
	
  
ID:	
  three	
  segmented	
  antennae	
  with	
  a	
  hair	
  protruding	
  from	
  end	
  (aristate)	
  
ID:	
  brown	
  calypters	
  (reduced	
  hind	
  wing)	
  just	
  in	
  front	
  of	
  small	
  light	
  haltêres	
  
Other:	
  long	
  proboscis,	
  red	
  large	
  compound	
  eyes,	
  small	
  clubbed	
  antennae	
  with	
  red	
  tip	
  	
  
Other:	
  White	
  and	
  black	
  abdomen	
  curled	
  downward	
  
Other	
  9mm	
  long	
  
(Kaufman	
  p.306)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
20	
  
B3	
  Bee	
  Fly	
  
Family:	
  Bombyliinae	
  
Genus:	
  Bombylius	
  
	
  
ID:	
  Clearly	
  a	
  bee	
  mimic,	
  thick	
  yellow	
  fur	
  on	
  thorax	
  and	
  abdomen	
  
ID:	
  long,	
  stiff	
  proboscis	
  like	
  a	
  hummingbird’s	
  bill	
  
ID:	
  long,	
  thin,	
  delicate	
  legs	
  
Other:	
  Large	
  brown	
  club-­‐shaped	
  haltêres,	
  10mm	
  long,	
  17mm	
  wingspan	
  
Other:	
  dark	
  on	
  top	
  of	
  wings	
  and	
  translucent	
  below	
  	
  
(Kaufman	
  p.294)	
  
	
  
B4	
  Bumble	
  Flower	
  Beetle	
  
Family:	
  Scarabaeidae	
  
Genus:	
  Euphoria	
  
	
  
ID:	
  broadly	
  oval,	
  yellow	
  hairs	
  covering	
  body,	
  dark	
  pattern,	
  loud	
  buzz	
  in	
  flight	
  
ID:	
  Antennae	
  end	
  with	
  three	
  fingerlike	
  projections	
  
Other:	
  8-­‐8mm	
  long	
  
Known	
  pollinators,	
  feed	
  at	
  flowers	
  
(Kaufman	
  p.138)	
  
	
  
	
  
	
  
	
  
21	
  
B5	
  Large	
  Carpenter	
  Bee	
  
Family:	
  Apidae	
  
Genus:	
  Xylocopa	
  
	
  
ID:	
  large	
  size	
  and	
  hair	
  placement,	
  black	
  dot	
  on	
  center	
  of	
  thorax	
  
Other:	
  20mm	
  long	
  
Other:	
  Furry	
  yellow	
  thorax	
  w/	
  black	
  spot	
  in	
  center,	
  furry	
  black	
  legs	
  
Other:	
  Hairless,	
  shiny	
  black	
  abdomen,	
  black	
  head,	
  segmented	
  antennae	
  
Other:	
  	
  Quickly	
  agitated,	
  buzzes	
  loudly,	
  shakes	
  abdomen	
  
(Kaufman	
  p.344)	
  
	
  
B6	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  
Other:	
  flattened	
  black	
  hairless	
  abdomen,	
  hair	
  on	
  thorax	
  and	
  back	
  legs	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
	
  
	
  
22	
  
	
  
B7	
  Red	
  Admiral	
  Butterfly	
  
Family:	
  Nymphalidae	
  
Genus:	
  Vanessa	
  
Species:	
  Vanessa	
  atalanta	
  
	
  
ID:	
  Wing	
  coloration,	
  dark	
  with	
  orange	
  streaks	
  and	
  white	
  dots	
  at	
  tips	
  of	
  top	
  pair	
  
Bottom	
  wings	
  brown,	
  top	
  has	
  orange,	
  black	
  and	
  white,	
  long	
  antennae	
  
20mm	
  body	
  length,	
  1	
  wing	
  is	
  30mm	
  head	
  to	
  tail	
  
(Kaufman	
  p.	
  234)	
  
	
  
B8	
  Sweat	
  Bee	
  
Family:	
  Halictus	
  
Genus:	
  Halictidae	
  
	
  
ID:	
  Thickened	
  yellow	
  back	
  legs,	
  yellow	
  hairs	
  on	
  part	
  of	
  thorax,	
  mostly	
  hairless	
  
Fuzzy	
  yellow	
  hind	
  legs,	
  yellow	
  fuzz	
  between	
  eye	
  and	
  antennae	
  
12mm	
  long,	
  body	
  black	
  and	
  shiny	
  
Wings	
  same	
  length	
  as	
  body,	
  long	
  thin	
  antennae	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
23	
  
B9	
  Honey	
  Bee	
  
Family:	
  Apidae	
  
Species:	
  Apis	
  mellifera	
  
	
  
ID:	
  body	
  is	
  the	
  color	
  of	
  honey	
  with	
  black	
  stripes	
  on	
  abdomen	
  
Other:	
  hair	
  on	
  thorax,	
  pollen	
  packets	
  on	
  hind	
  legs	
  
(Kaufman	
  p.347)	
  
	
  
B10	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
Genus:	
  Andrena	
  
	
  
ID:	
  hairs	
  between	
  compound	
  eyes	
  and	
  antennal	
  base	
  
Other:	
  shaking	
  abdomen,	
  rubbing	
  hind	
  legs	
  together	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
	
  
	
  
24	
  
B11	
  Flower	
  Fly	
  1	
  
Family:	
  Syrphidae	
  
Genus:	
  Unknown,	
  possibly	
  Mallota	
  
	
  
ID:	
  spurious	
  vein	
  which	
  is	
  found	
  parallel	
  to	
  fourth	
  longitudinal	
  wing	
  vein	
  
Bee	
  mimic,	
  15mm	
  long,	
  2	
  short	
  antennae	
  with	
  terminal	
  black	
  hair	
  
Other:	
  Lower	
  mandible	
  protrudes,	
  slight	
  downward	
  carve	
  to	
  abdomen	
  
(Kaufman	
  p.300)	
  
	
  
B12	
  Flower	
  Fly	
  2	
  
Family:	
  Syrphidae	
  
Genus:	
  Unknown,	
  somewhat	
  like	
  Platycherius	
  &	
  Xylota	
  but	
  wider	
  abdomen	
  
	
  
ID:	
  :	
  spurious	
  vein	
  which	
  is	
  found	
  parallel	
  to	
  fourth	
  longitudinal	
  wing	
  vein	
  
9mm	
  long	
  body,	
  short	
  antennae	
  ending	
  in	
  a	
  hair,	
  black	
  eyes,	
  white	
  face	
  
Rubbing	
  front	
  legs,	
  very	
  flat	
  abdomen	
  slightly	
  curved	
  down	
  
Thorax	
  has	
  fine	
  hairs,	
  two	
  light	
  spots	
  on	
  both	
  abdomen	
  sides	
  
(Kaufman	
  p.302)	
  
	
  
	
  
	
  
25	
  
C1	
  Plant	
  Bug	
  
Family:	
  Miridae	
  
	
  
ID:	
  distinct	
  membrane	
  and	
  corium,	
  cuneus	
  present	
  
Other:	
  5mm	
  long,	
  not	
  a	
  pollinator	
  
(Kaufman	
  p.	
  113)	
  
	
  
C2	
  Spread-­‐winged	
  Damselfly	
  
Family:	
  Lestidae	
  
	
  
ID:	
  arculus	
  is	
  broader	
  than	
  narrow-­‐winged	
  damselfly	
  
Other:	
  33mm	
  long,	
  black	
  and	
  pale	
  blue	
  vertical	
  stripes,	
  wings	
  slightly	
  spread	
  while	
  resting	
  
(Kaufman	
  p.52)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
26	
  
C3	
  Sweat	
  Bee	
  
Family:	
  Halictidae	
  
	
  
ID:	
  ID:	
  Entire	
  body	
  metallic	
  green	
  and	
  slightly	
  hairy	
  
Other:	
  three	
  caught,	
  only	
  one	
  recorded	
  
Other:	
  Fuzzy	
  legs,	
  short	
  hairs,	
  10mm	
  long	
  
1	
  sub-­‐antennal	
  suture	
  under	
  each	
  antennal	
  socket,	
  
basal	
  vein	
  arched	
  
(Kaufman	
  p.340)	
  	
  
	
  
C4	
  Mining	
  Bee	
  
Family:	
  Andrenidae	
  
	
  
ID:	
  fellow	
  hairy	
  patch	
  between	
  eye	
  and	
  antenna	
  
Other:	
  hairy	
  yellow	
  thorax,	
  shiny	
  black	
  abdomen,	
  12	
  mm	
  long	
  
(Kaufman	
  p.340)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
27	
  
C5	
  Rove	
  Beetle	
  1	
  
Family:	
  Staphylinidae	
  
	
  
ID:	
  Antennae	
  longer	
  than	
  body,	
  filamentous,	
  slender	
  body,	
  shortened	
  elytra	
  
ID:	
  antennae	
  not	
  clubbed,	
  narrow	
  waist,	
  noticeable	
  claws	
  
Other:	
  6mm	
  long	
  
(Kaufman	
  p.200)	
  
	
  
C6	
  Rove	
  Beetle	
  2	
  
Family:	
  Staphylinidae	
  
Genus:	
  
	
  
ID:	
  very	
  small	
  elytra,	
  no	
  projections	
  on	
  antennae,	
  narrow	
  waist,	
  distinctive	
  claws	
  
ID	
  yellow	
  legs	
  with	
  thick	
  knees,	
  black	
  body	
  
Other	
  8.5mm	
  long	
  
(Kaufman	
  p.200)	
  
	
  
	
  
	
  
	
  
	
  
28	
  
C7	
  Assassin	
  Bug	
  
Family:	
  Reduviidae	
  
	
  
ID:	
  black	
  wings,	
  green	
  body,	
  long	
  antennae,	
  6	
  legs,	
  black	
  spot	
  on	
  joints	
  
Other	
  red	
  eyes,	
  some	
  white	
  on	
  belly,	
  13mm	
  long	
  
(Kaufman	
  p.	
  116)	
  
	
  
C8	
  Yellowjacket	
  
Family:	
  Vespidae	
  
Genus:	
  Vespula	
  
	
  
ID:	
  Dark	
  wings,	
  thorax	
  has	
  2	
  yellow	
  bars	
  and	
  2	
  yellow	
  spots	
  on	
  bottom	
  
ID:	
  antennae	
  are	
  black	
  with	
  2	
  segments,	
  smoothed	
  stinger	
  (female?)	
  
ID:	
  body	
  is	
  smooth	
  no	
  fuzz	
  
Most	
  likely	
  Eastern	
  Yellowjacket,	
  Vespula	
  maculifrons,	
  due	
  to	
  pattern	
  and	
  range	
  
(Kaufman	
  p.357)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
29	
  
C10	
  Bumble	
  Flower	
  Beetle	
  2	
  
Family:	
  Scarabaeidae	
  
Genus:	
  Trichiotinus	
  
	
  
ID:	
  end	
  of	
  antennae	
  are	
  a	
  club	
  with	
  3	
  parts,	
  pronged	
  
ID:	
  elytra	
  very	
  short,	
  does	
  not	
  cover	
  body	
  
Other:	
  7.5mm	
  
(Kaufman	
  p.139)	
  
	
  
C11	
  Sweat	
  Bee	
  
Family:	
  Halictidae	
  
Genus:	
  Lasioglossum	
  
	
  
ID:	
  Body	
  is	
  slightly	
  metallic	
  green,	
  hairy	
  yellow	
  legs,	
  slender,	
  flat	
  abdomen,	
  glossy	
  
Other:	
  7mm	
  long	
  
(Kaufman	
  p.	
  341)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
30	
  
C12-­‐	
  Spread-­‐winged	
  Damselfly,	
  released	
  without	
  further	
  documentation	
  
C13-­‐	
  Red	
  Admiral,	
  released	
  without	
  further	
  documentation	
  
C14	
  Flesh	
  Fly	
  
Family:	
  Sarcophagidae	
  
	
  
ID:	
  one	
  pair	
  wings,	
  hairy	
  thorax	
  with	
  black	
  and	
  silver	
  vertical	
  stripes	
  
Other:	
  large	
  brown	
  eyes,	
  pale	
  face,	
  10mm	
  long	
  
(Kaufman	
  p.306)	
  
	
  
C15	
  Thread-­‐Waisted	
  Wasp	
  
Family:	
  Sphecidae	
  
Genus:	
  Crabro	
  
	
  
ID:	
  small,	
  slender	
  
ID:	
  yellow	
  stripes	
  on	
  black	
  abdomen,	
  yellow	
  shapes	
  on	
  black	
  thorax,	
  hourglass	
  shape	
  body	
  
ID:	
  yellow	
  neck	
  ring,	
  yellow	
  hairs	
  on	
  forehead,	
  dark	
  patch	
  on	
  outer	
  wing	
  
Other:	
  9mm	
  long	
  
(Kaufman	
  p.338)	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
	
  
31	
  
D1	
  Ichneumon	
  Wasp,	
  Scorpion	
  Wasp	
  
Family:	
  Ichneumonidae	
  
	
  
ID:	
  antennae>10mm,	
  body<10mm,	
  back	
  leg	
  pair>10mm	
  
ID:	
  yellow	
  back	
  legs	
  with	
  black	
  joints,	
  honey	
  brown	
  abdomen	
  thinly	
  connected	
  to	
  thorax	
  
ID:	
  many	
  antennal	
  segments,	
  all	
  black	
  
(Kaufman	
  p.	
  326)	
  
	
  
D2	
  Syrphid	
  Fly	
  	
  
Family:	
  Syrphidae	
  
Genus:	
  Toxomerus	
  
Species:	
  Toxomerus	
  germinatus	
  
	
  
ID:	
  Very	
  distinctive	
  arrow	
  pattern	
  on	
  back	
  
matches	
  Toxomerus	
  germinatus,	
  within	
  range	
  
ID:	
  White,	
  small	
  club	
  haltêres	
  
ID:	
  Wing	
  pattern	
  exactly	
  matches	
  this	
  species	
  
ID:	
  extremely	
  short	
  antennae	
  with	
  hair	
  
Other:	
  pale	
  underside,	
  large	
  eyes	
  fill	
  face	
  
(Kaufman	
  p.298,	
  303)	
  
	
  
	
  
	
  
	
  
	
  

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Hawthorn Pollinator Report_Snow

  • 1.     Hawthorn  Pollinator  Examination   Jessica  Snow   Biol  320  Sec  001   08  VI  2015   All  photos  by  Jessica  Snow   Data  Collected  by  Anna  Wan,  Sam  Walker,  and  Jessica  Snow        
  • 2. 2   Table  of  Contents:               Introductions_______________________________________________________________________________________3   Methods_____________________________________________________________________________________________3   Results_______________________________________________________________________________________________4   Discussion___________________________________________________________________________________________6   Key  for  Basic  Identification________________________________________________________________________7   Conclusion_________________________________________________________________________________________10   References_________________________________________________________________________________________11   Appendix  A________________________________________________________________________________________12        
  • 3. 3   Introduction   Pollination,  primarily  by  insects,  is  one  of  the  most  important  ecological  services   provided  by  a  group  of  organisms.  Pollination  is  key  to  sexual  reproduction  in  many   flowering  plants,  and  allows  for  the  continued  introduction  of  variation  by  genetic   recombination  (Clancy).  This  genetic  variation  is  vital  to  stable  communities,  and  with   variation  comes  the  ability  to  survive  environmental  changes.  In  addition,  pollination  is   needed  for  the  production  of  35%  of  the  crops  used  for  food  worldwide  (Insects  &   Pollinators).  With  this  in  mine,  it  becomes  apparent  how  crucial  it  is  to  support  native   pollinator  species.  Genus  Crataegus,  or  commonly  called,  Hawthorn  trees  have  multiple   species  native  to  Michigan  (Michigan  Flora  Online).  Many  of  these  trees  are  known  to  help   support  other  species  by  providing  food  and  shelter.  In  our  study,  we  surveyed  the   individuals  visiting  Hawthorn  trees  while  in  bloom.  This  survey  shows  Hawthorn  tree’s   ability  to  support  local  pollinator  species  by  providing  them  with  a  seasonal  food  source.       Methods       To  learn  about  the  diversity  of   pollinators  that  visit  the  Hawthorn  trees  in   Jensen’s  meadow,  collections  were  made  on   four  days  from  two  sites.  Both  sites  of   collection  were  adult  Hawthorn  trees  found   in  Jensen’s  meadow  on  the  University  of   Dearborn  Campus  Natural  Area  (figure  1).   Collection  periods  were  conducted  in  late   morning  to  achieve  best  collection  condition.   Goal  conditions  were  full  sun,  calm,  and   warm.  Due  to  natural  weather  variation,  not   all  collections  could  be  made  in  these  prime  conditions  (table  1).  On  May  22,  collection   techniques  were  practiced  and  no  specimens  were  analyzed.  Specimens  were  collected   using  fine  mesh  nets,  transferred  into  plastic  transfer  containers  and  stored  in  a  shaded   region  until  the  collection  was  complete.  During  collection,  repeat  specimens  were  not   collected  if  possible.  Collection  focused  on  examining  the  diversity,  not  quantity,  of     Figure  1:  Map  of  Collection  Area  
  • 4. 4   pollinators  visiting  the  Hawthorn  trees.    After  collection  was  competed,  specimens  were   refrigerated  for  20-­‐35m  and  then  transferred  to  individual  vials  for  observation.  These   vials  were  kept  refrigerated  or  in  ice  water  when  not  being  analyzed  to  inhibit  movement   and  stress  of  specimens  collected.  They  were  then  observed  under  a  dissecting  microscope   and  photographed.  A  record  was  taken  of  each  identified  pollinator.  To  help  in   identification,  Kaufman’s  Field  Guide  of  Insects  and  BugGuide.net  were  used.  Collection   ended  with  the  end  of  the  blooming  period  for  the  Hawthorn  trees,  which  bloomed  for  just   over  one  week.  Organisms  were  identified  to  their  family,  a  few  were  identified  to  genus,   some  were  tentatively  identified  to  genus,  and  two  were  identified  to  species.  In  analysis  of   diversity,  different  specimens  in  the  same  family  were  only  considered  different  if  they   were  morphologically  unique.  In  this  case,  they  were  given  a  number  after  their  name  to   denote  this  difference  (table  2,  appendix  A).     Results     From  four  days  of  collection,  25  unique  organisms  were  identified  to  be  visiting  the   Hawthorn  trees.  Of  these  species,  not  all  are  known  to  collect  pollen  or  nectar,  instead   using  the  Hawthorn  for  other  purposes  such  as  feeding  on  plants  and  insects.  These  will  be   considered  secondary  pollinators  and  are  denoted  in  table  2  with  an  asterisk.  Kaufman’s   Filed  Guide  to  insects  was  used  to  make  this  determination  (Kaufman).  To  see  further   details  about  behaviors  that  qualified  organisms  as  primary  or  secondary  pollinators,  refer   to  Appendix  A.  In  total,  17  commonly  considered  pollinators,  which  we  will  call  primary   pollinators,  were  identified.  8  secondary  pollinators  were  collected.  Many  secondary   pollinators  accidentally  pollinate  flowers  while  performing  their  own  feeding  behavior.   Day   Test   A   B   C   D   Date   22  V  2015   23  V  2015   27  V  2015   29  V  2015   01  V1  2015   Time  (EST)   1230-­‐0130   1046-­‐1130   0905-­‐1005   0920-­‐1020   1220-­‐1250   Hawthorn  sampled     A   A   A   A   B   Temperature  (°C)   N/A   25.9   26.0   25.6   13   Humidity  (%)   N/A   32   61   78   58   Sky  cover   N/A   Clear   Clear   Full  cloud   Full  cloud   Wind   N/A   Calm   Calm   Calm   1.5mph   Analysis  Time   N/A   1130-­‐0300   1205-­‐0305   1210-­‐0310   1250-­‐0150   Bloom  Stage   Early     Middle   Middle   Fading   Late   Table  1:  Collection  Information  
  • 5. 5   This  fact    was  determined  from  the  visible  presence  of  pollen  grains  on  many  of  these   individuals.  Day  B,  May  27th  had  the  best  collecting  conditions  and  was  the  time  of  fullest   flower  blooms.  This  day  had  sunshine  and  high  temperatures,  which  seemed  to  encourage   pollination  behavior.  Day  B  was  a  diverse  collection  day,  and  the  majority  of  species   collected  were  known  pollinators.  On  day  B,  10  different  types  of  pollinators  were  collected   and  90%  were  primary  pollinators  (table  2).  On  day  C,  12  types  of  pollinators  were   collected,  but  only  50%  were  primary  pollinators.  Day  A  was  much  less  diverse  with  only  6   types  collected,  80%  of  them  being  primary  pollinators.  The  day  of  lowest  diversity  was   day  D,  with  only  two  individuals  collected,  both  being  primary  pollinators.  This  could  be   due  both  to  cold  cloudy  weather  and  the  end  of  bloom  conditions.  Mining  bees  were     23  V  2015   27  V  2015   29  V  2015   01  V1  2015   Small  Carpenter  Bee   II         Mining  Bee   IIII   III   I     Asian  Multicolored  Lady  Beetle*   I         Spotted  Lady  Beetle   I         Metallic  Sweat  Bee   II         Flesh  Fly   I     I     Muscid  Fly*     I       Bee  Fly     I       Bumble  Flower  Beetle     I       Large  Carpenter  Bee     I       Red  Admiral  Butterfly     I   I     Sweat  Bee     I   II     Honey  Bee     I       Flower  Fly  1     I       Flower  Fly  2     I       Plant  Bug*       I     Spread-­‐winged  Damselfly*       II     Rove  Beetle  1*       I     Rove  Beetle  2*       I     Assassin  Bug*       I     Yellowjacket       I     Bumble  Flower  Beetle  1       I     Thread-­‐waisted  Wasp*       I     Ichneumon  Wasp         I   Syrphid  Fly           I   Table  2:  Collection  Results   *Secondary  Pollinator  
  • 6. 6   collected  on  three  of  the  four  collection  days,  making  them  a  common  pollinator  of   Hawthorns.  Flesh  flies,  Red  Admiral  butterflies,  and  Sweat  bees  were  all  collected  on  two   occasions,  meaning  that  they  may  be  somewhat  common  pollinators  for  Hawthorn  trees.     Discussion     The  taxa  richness  observed  at  the  Hawthorn  trees  during  the  4  days  of  collection   show  that  they  are  host  to  many  different  pollinators.    It  can  be  surmised  that  Hawthorn   trees  are  capable  of  helping  to  support  a  largely  diverse  population  of  pollinators.  This   allows  for  the  opportunity  to  introduce  Hawthorn  trees  to  an  area  where  the  pollinators   found  in  this  study  would  be  a  positive  addition.  In  habitat  management,  this  data  can  be   used  as  a  guide  to  look  at  benefits  and  drawbacks  of  adding  Hawthorn  trees  into  an   environment.     Methods  of  identification  were  learned  by  trial  and  error  during  the  4  days  of   collection.    The  Key  below  was  created  as  a  summary  of  the  best  techniques  acquired.  In   our  identification,  the  first  necessary  step  was  to  isolate  the  invertebrates  into  broad   categories.  After  that  was  determined  more  specific  classifications  were  made.       Key  For  Basic  Identification   1. Can  haltêres  (small  knob  structures)  be  identified  behind  the  front  wing?   a. If  yes,  you  have  a  fly.  Flies  only  have  one  set  of  wings  which  often  fold  in.     Example  of  a  fly  (left)  and  close-­‐up  of  haltêre  (right)   b. If  no,  continue  to  question  two.  
  • 7. 7     2. Can  elytra  (hardened  wing  cover)  be  observed  in  rest,  often  concealing  wings?   a. If  yes,  you  have  a  beetle.       Examples  of  two  types  of  beetles  with  hard  wing-­‐covers  (above)   b. If  no,  continue  on  to  question  three.   3. Are  there  broad  wings,  more  than  3  times  the  size  of  body?   a. If  yes,  you  have  a  butterfly,  or  moth.     Examples  of  a  Red  Admiral  Butterfly  (above)   b. If  no,  continue  on  to  question  four.        
  • 8. 8       4. Is  the  abdomen  very  narrow,  2  pairs  of  wings  long,  and  body  often  blue  or  green?   a. If  yes,  you  have  a  damselfly  or  pondhawk     Example  of  Spread-­‐winged  Damselfly  (above)   b. If  no,  continue  on  to  question  five.   5. Are  legs  much  longer  than  body,  highly  jointed,  or  body  with  highly  angular  shape?   a. If  yes,  you  may  have  a  plant  bug,  assassin  bug  or  other  secondary  pollinator.     Example  of  plant  bug  (left)  and  assassin  bug  (right)   b. If  no,  continue  on  to  question  six.      
  • 9. 9     6. Is  connection  between  thorax  and  abdomen  very  narrow  or  hourglass  shaped?   a. If  yes,  you  may  have  a  wasp  or  similar  family     Example  of  Thread-­‐Waisted  (Left)  and  Ichneumon  Wasp  (right)   b. If  no,  you  may  have  a  bee  if  it  fits  these  criterion:  hairy  or  not,  shiny  or  not,   compound  eyes  on  sides  of  head,  antennae  usually  moderately  long,  legs   thicker,  joined  and  shorter  than  body  length.  Holds  wings  to  body  when  at   rest.  Yellowjackets  have  yellow  and  black  distinct  patterning  on  abdomen.     Example  of  sweat  bee  (left)  and  yellowjacket  (right)   This  experience  showed  that  insect  identification  is  not  black  and  white,  which  is   why  identification  keys  are  tricky  to  make.  The  purpose  of  the  above  dichotomous  key  is  to   help  identify  common  pollinators  found  at  Hawthorn  trees,  and  was  the  approach  
  • 10. 10   developed  during  identification.  The  key  can  be  used  as  a  good  way  to  start  figuring  out   what  type  of  arthropod  you  are  dealing  with.  The  rules  above  will  have  exceptions  and   won’t  be  accurate  in  all  cases.  It  is  hoped  that  with  resources  for  easier  identification,   important  pollinators  will  be  recognized  and  appreciated  for  their  high  ecological  value  as   well  as  for  intrinsic  value.   This  study  was  completed  in  hopes  that  further  research  will  be  conducted  on  the   same  and  other  local  species  that  support  diverse  and  beneficial  pollinator  populations.   This  study  should  be  followed  up  with  the  following  considerations.  The  collection  may   have  been  biased  to  attractive,  moving,  loud  pollinators  and  therefore  a  representative   sample  may  not  have  been  collected.  Repeat  observations  need  to  be  conducted  to  see  if  the   recorded  pollinator  population  is  consistent  on  a  yearly  basis.  Further  analysis  of  identified   pollinators  should  occur  to  further  classify  and  understand  the  diversity  of  pollinators   supported  by  Hawthorn  trees.       Conclusion   The  survey  of  pollinators  at  Hawthorn  trees  shows  signs  of  high  diversity,   suggesting  that  Hawthorns  are  important  members  of  a  community  that  is  able  to  support   pollinators.  By  supporting  many  pollinating  organisms,  addition  of  Hawthorn  trees  into   meadow  environments  is  recommended  when  other  flower  plants  exist  to  support  the   pollinator  population  for  the  355  days  of  the  year  when  Hawthorn’s  are  not  in  bloom.  In   this  way,  Hawthorns  can  contribute  to  a  healthy  community  and  provide  a  stable  food   source  for  these  pollinators.  This  study  can  help  initiate  further  research  into  local   pollinator  populations  and  the  plant  species  that  support  them,  helping  us  understand  the   biotic  relationships  that  support  the  communities  and  populations  we  live  in.  This  will   allow  us  to  make  educated  and  responsible  decisions  about  habitat  management.      
  • 11. 11   References     Kaufman,  Kenn,  and  Eric  R.  Eaton.  Kaufman  Field  Guide  to  Insects  of  North  America.  New   York,  NY:  Houghton  Mifflin,  2007.  Print.   BugGuide.  Iowa  State  University,  Department  of  Entomology,  2015.  Web.  08  June  2015.   <http://bugguide.net/>.   Michigan  Flora  Online.  A.  A.  Reznicek,  E.  G.  Voss,  &  B.  S.  Walters.  February  2011.  University   of  Michigan.  Web.  June  8,  2015.  http://michiganflora.net/genus.aspx?id=Crataegus.   Clancy,  S.  (2008)  Genetic  recombination.  Nature  Education  1(1):40  <   http://www.nature.com/scitable/topicpage/genetic-­‐recombination-­‐514>.   "Insects  &  Pollinators."  Natural  Resources  Conservation  Service.  United  States  Department   of  Agriculture,  2015.  Web.  08  June  2015.  <http://www.nrcs.usda.gov/wps/portal/   nrcs/main/national/plantsanimals/pollinate/>.      
  • 12. 12   Appendix  A:    Behavior  Information  used  to  Determine  Primary  vs.  Secondary  Pollinators       Diet  or  Behavioral  Trail   Small  Carpenter  Bee   Thistles  and  other  flowers   Mining  Bee   Pollen  and  nectar  from  specific  flowers   Asian  Multicolored  Lady  Beetle*   Aphids   Spotted  Lady  Beetle   Eats  aphids  and  pollen   Metallic  Sweat  Bee   Known  to  pollinate  flowers   Flesh  Fly   Adults  eat  flower  nectar   Muscid  Fly*   Adults  feed  on  living  plant  or  animal  tissue   Bee  Fly   Eats  nectar  of  lilac,  plum  and  other  flowers   Bumble  Flower  Beetle   Feed  on  pollen,  nectar,  fruit  and  plant  juices   Large  Carpenter  Bee   Pollinate  apples,  pears  and  other  fruit   Red  Admiral  Butterfly   Adults  eat  fruit,  sap,  bird  droppings  and  nectar   Sweat  Bee   Known  to  pollinate  flowers   Honey  Bee   Pollinate  flowers   Flower  Fly  1   Feed  on  many  flower  parts   Flower  Fly  2   Feed  on  many  flower  parts   Plant  Bug*   Feed  on  plants,  some  are  predatory   Spread-­‐winged  Damselfly*   Feed  on  insects   Rove  Beetle  1*   Mostly  insect  eaters   Rove  Beetle  2*   Mostly  insect  eaters   Assassin  Bug*   Eat  insects   Yellowjacket   Adults  collect  flower  nectar   Bumble  Flower  Beetle  2   Feed  on  pollen,  nectar,  fruit  and  plant  juices   Thread-­‐waisted  Wasp*   Eat  flies   Ichneumon  Wasp   Some  are  parasites,  others  visit  flowers   Syrphid  Fly     Feed  on  flower  parts   *Gray  and  asterisks  means  the  organism  has  been  determined  to  be  a  secondary  pollinator.                        
  • 13. 13   Appendix  B:     Species  List     Methods  of  Collection   1. Collected  specimens  only  that  landed  on  Hawthorn  tree  near  flower  or  hover  nearby   2. Moths  and  caterpillars  released   3. Smallest  insects  could  not  be  captured  using  our  methods   4. Repeat  specimen  collections  for  a  day  were  avoided  when  detected   5. Stored  in  plastic  containers  in  shade  during  collection   6. Collections  limited  to  one  hour     7. Specimen  were  stored  in  refrigerator  20min  or  more  before  analysis   8. Identified  used  dissecting  microscopes  and  a  camera   9. Identified  specimens  were  released  S.  of  EIC         A1  Small  Carpenter  Bee   Family:  Apidae   Genus:  Ceratina     ID:  7cm  long,   ID:  head  and  thorax  iridescent  dark  green,  almost  black   ID:  very  few  hairs  on  head,  abdomen  and  thorax   ID:  Distinguished  from  small  sweat  bees  because  less  hair  and  lobed  back  wing   Pollen  hairs  on  3rd  pair  of  legs  only   Slender  body   Small  mouthparts   Long  slender  wings   (Kaufman  p.348)        
  • 14. 14   A2  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base   ID:  very  long  hairs  on  3rd  pair  of  legs   (Kaufman  p.340)     A3  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base     Long,  slender  wings   Yellow  hairs  on  full  body  and  legs   10mm  long   (Kaufman  p.340)                
  • 15. 15   A4  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base     (Kaufman  p.340)     A5  Small  Carpenter  Bee   Family:  Apidae   Genus:  Ceratina     ID:  slender  full  iridescent  green  body   ID:  very  few  hairs  on  head,  abdomen  and  thorax   Pollen  in  long  hairs  on  3rd  leg  pair   8.5mm  long   (Kaufman  p.348)                  
  • 16. 16   A6  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base     (Kaufman  p.340)     A7  Asian  Multicolored  Lady  Beetle   Family:  Coccinellidae   Species:  Harmonia  axyridis     ID:  Black  M-­‐shaped  pattern  on  white  pronotum   ID:  Elytra  orange,  shiny  with  black  spots   ID:  a  beetle  due  to  hardened  elytra   (Kaufman  p.156)              
  • 17. 17   A8  Spotted  Lady  Beetle   Family:  Coccinellidae     Species:  coleomegilla  maculata     ID:  Elytra  orange  with  12  large  black  spots,  pronotum  orange  with  two  black  spots   ID:  flattened  back,  long  abdomen,  black  legs   ID:  a  beetle  due  to  hardened  elytra   (Kaufman  p.154)     A9  Metallic  Sweat  Bee   Family:  Halictidae   Genus:  probably  Augochlora  or  Augochlorella     ID:  Entire  body  metallic  green  and  hairy   Fine  yellow  hairs  on  all  legs   Abdomen  slender,  curved  down   9.5mm  long   (Kaufman  p.340)           A10  Released,  Not  a  Pollinator    
  • 18. 18   A11  Flesh  Fly   Family:  Sarcophagidae     ID:  one  pair  wings,  white  large  haltêres   ID:  short  antennae   Red-­‐brown  eyes,  thorax  with  black  and  silver  vertical  stripes,  hairy   Feet  with  two  pads,  wings  lay  flat   (Kaufman  p.306)     A12  Metallic  Sweat  Bee   Family:  Halictidae     ID:  Entire  body  metallic  green  and  hairy,  two  sets  of  wings   Abdomen  eaten  by  other  insect   (Kaufman  p.340)              
  • 19. 19   B1  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base   (Kaufman  p.340)     B2  Muscid  Fly   Family:  Muscidae     ID:  three  segmented  antennae  with  a  hair  protruding  from  end  (aristate)   ID:  brown  calypters  (reduced  hind  wing)  just  in  front  of  small  light  haltêres   Other:  long  proboscis,  red  large  compound  eyes,  small  clubbed  antennae  with  red  tip     Other:  White  and  black  abdomen  curled  downward   Other  9mm  long   (Kaufman  p.306)                  
  • 20. 20   B3  Bee  Fly   Family:  Bombyliinae   Genus:  Bombylius     ID:  Clearly  a  bee  mimic,  thick  yellow  fur  on  thorax  and  abdomen   ID:  long,  stiff  proboscis  like  a  hummingbird’s  bill   ID:  long,  thin,  delicate  legs   Other:  Large  brown  club-­‐shaped  haltêres,  10mm  long,  17mm  wingspan   Other:  dark  on  top  of  wings  and  translucent  below     (Kaufman  p.294)     B4  Bumble  Flower  Beetle   Family:  Scarabaeidae   Genus:  Euphoria     ID:  broadly  oval,  yellow  hairs  covering  body,  dark  pattern,  loud  buzz  in  flight   ID:  Antennae  end  with  three  fingerlike  projections   Other:  8-­‐8mm  long   Known  pollinators,  feed  at  flowers   (Kaufman  p.138)          
  • 21. 21   B5  Large  Carpenter  Bee   Family:  Apidae   Genus:  Xylocopa     ID:  large  size  and  hair  placement,  black  dot  on  center  of  thorax   Other:  20mm  long   Other:  Furry  yellow  thorax  w/  black  spot  in  center,  furry  black  legs   Other:  Hairless,  shiny  black  abdomen,  black  head,  segmented  antennae   Other:    Quickly  agitated,  buzzes  loudly,  shakes  abdomen   (Kaufman  p.344)     B6  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base   Other:  flattened  black  hairless  abdomen,  hair  on  thorax  and  back  legs   (Kaufman  p.340)              
  • 22. 22     B7  Red  Admiral  Butterfly   Family:  Nymphalidae   Genus:  Vanessa   Species:  Vanessa  atalanta     ID:  Wing  coloration,  dark  with  orange  streaks  and  white  dots  at  tips  of  top  pair   Bottom  wings  brown,  top  has  orange,  black  and  white,  long  antennae   20mm  body  length,  1  wing  is  30mm  head  to  tail   (Kaufman  p.  234)     B8  Sweat  Bee   Family:  Halictus   Genus:  Halictidae     ID:  Thickened  yellow  back  legs,  yellow  hairs  on  part  of  thorax,  mostly  hairless   Fuzzy  yellow  hind  legs,  yellow  fuzz  between  eye  and  antennae   12mm  long,  body  black  and  shiny   Wings  same  length  as  body,  long  thin  antennae   (Kaufman  p.340)          
  • 23. 23   B9  Honey  Bee   Family:  Apidae   Species:  Apis  mellifera     ID:  body  is  the  color  of  honey  with  black  stripes  on  abdomen   Other:  hair  on  thorax,  pollen  packets  on  hind  legs   (Kaufman  p.347)     B10  Mining  Bee   Family:  Andrenidae   Genus:  Andrena     ID:  hairs  between  compound  eyes  and  antennal  base   Other:  shaking  abdomen,  rubbing  hind  legs  together   (Kaufman  p.340)              
  • 24. 24   B11  Flower  Fly  1   Family:  Syrphidae   Genus:  Unknown,  possibly  Mallota     ID:  spurious  vein  which  is  found  parallel  to  fourth  longitudinal  wing  vein   Bee  mimic,  15mm  long,  2  short  antennae  with  terminal  black  hair   Other:  Lower  mandible  protrudes,  slight  downward  carve  to  abdomen   (Kaufman  p.300)     B12  Flower  Fly  2   Family:  Syrphidae   Genus:  Unknown,  somewhat  like  Platycherius  &  Xylota  but  wider  abdomen     ID:  :  spurious  vein  which  is  found  parallel  to  fourth  longitudinal  wing  vein   9mm  long  body,  short  antennae  ending  in  a  hair,  black  eyes,  white  face   Rubbing  front  legs,  very  flat  abdomen  slightly  curved  down   Thorax  has  fine  hairs,  two  light  spots  on  both  abdomen  sides   (Kaufman  p.302)        
  • 25. 25   C1  Plant  Bug   Family:  Miridae     ID:  distinct  membrane  and  corium,  cuneus  present   Other:  5mm  long,  not  a  pollinator   (Kaufman  p.  113)     C2  Spread-­‐winged  Damselfly   Family:  Lestidae     ID:  arculus  is  broader  than  narrow-­‐winged  damselfly   Other:  33mm  long,  black  and  pale  blue  vertical  stripes,  wings  slightly  spread  while  resting   (Kaufman  p.52)                  
  • 26. 26   C3  Sweat  Bee   Family:  Halictidae     ID:  ID:  Entire  body  metallic  green  and  slightly  hairy   Other:  three  caught,  only  one  recorded   Other:  Fuzzy  legs,  short  hairs,  10mm  long   1  sub-­‐antennal  suture  under  each  antennal  socket,   basal  vein  arched   (Kaufman  p.340)       C4  Mining  Bee   Family:  Andrenidae     ID:  fellow  hairy  patch  between  eye  and  antenna   Other:  hairy  yellow  thorax,  shiny  black  abdomen,  12  mm  long   (Kaufman  p.340)                  
  • 27. 27   C5  Rove  Beetle  1   Family:  Staphylinidae     ID:  Antennae  longer  than  body,  filamentous,  slender  body,  shortened  elytra   ID:  antennae  not  clubbed,  narrow  waist,  noticeable  claws   Other:  6mm  long   (Kaufman  p.200)     C6  Rove  Beetle  2   Family:  Staphylinidae   Genus:     ID:  very  small  elytra,  no  projections  on  antennae,  narrow  waist,  distinctive  claws   ID  yellow  legs  with  thick  knees,  black  body   Other  8.5mm  long   (Kaufman  p.200)            
  • 28. 28   C7  Assassin  Bug   Family:  Reduviidae     ID:  black  wings,  green  body,  long  antennae,  6  legs,  black  spot  on  joints   Other  red  eyes,  some  white  on  belly,  13mm  long   (Kaufman  p.  116)     C8  Yellowjacket   Family:  Vespidae   Genus:  Vespula     ID:  Dark  wings,  thorax  has  2  yellow  bars  and  2  yellow  spots  on  bottom   ID:  antennae  are  black  with  2  segments,  smoothed  stinger  (female?)   ID:  body  is  smooth  no  fuzz   Most  likely  Eastern  Yellowjacket,  Vespula  maculifrons,  due  to  pattern  and  range   (Kaufman  p.357)                        
  • 29. 29   C10  Bumble  Flower  Beetle  2   Family:  Scarabaeidae   Genus:  Trichiotinus     ID:  end  of  antennae  are  a  club  with  3  parts,  pronged   ID:  elytra  very  short,  does  not  cover  body   Other:  7.5mm   (Kaufman  p.139)     C11  Sweat  Bee   Family:  Halictidae   Genus:  Lasioglossum     ID:  Body  is  slightly  metallic  green,  hairy  yellow  legs,  slender,  flat  abdomen,  glossy   Other:  7mm  long   (Kaufman  p.  341)                        
  • 30. 30   C12-­‐  Spread-­‐winged  Damselfly,  released  without  further  documentation   C13-­‐  Red  Admiral,  released  without  further  documentation   C14  Flesh  Fly   Family:  Sarcophagidae     ID:  one  pair  wings,  hairy  thorax  with  black  and  silver  vertical  stripes   Other:  large  brown  eyes,  pale  face,  10mm  long   (Kaufman  p.306)     C15  Thread-­‐Waisted  Wasp   Family:  Sphecidae   Genus:  Crabro     ID:  small,  slender   ID:  yellow  stripes  on  black  abdomen,  yellow  shapes  on  black  thorax,  hourglass  shape  body   ID:  yellow  neck  ring,  yellow  hairs  on  forehead,  dark  patch  on  outer  wing   Other:  9mm  long   (Kaufman  p.338)                    
  • 31. 31   D1  Ichneumon  Wasp,  Scorpion  Wasp   Family:  Ichneumonidae     ID:  antennae>10mm,  body<10mm,  back  leg  pair>10mm   ID:  yellow  back  legs  with  black  joints,  honey  brown  abdomen  thinly  connected  to  thorax   ID:  many  antennal  segments,  all  black   (Kaufman  p.  326)     D2  Syrphid  Fly     Family:  Syrphidae   Genus:  Toxomerus   Species:  Toxomerus  germinatus     ID:  Very  distinctive  arrow  pattern  on  back   matches  Toxomerus  germinatus,  within  range   ID:  White,  small  club  haltêres   ID:  Wing  pattern  exactly  matches  this  species   ID:  extremely  short  antennae  with  hair   Other:  pale  underside,  large  eyes  fill  face   (Kaufman  p.298,  303)